Penclomedine-induced DNA fragmentation and p53 accumulation correlate with reproductive cell death in colorectal carcinoma cells with altered p53 status.

1999 ◽  
Author(s):  
E Rakovitch ◽  
W Mellado ◽  
E J Hall ◽  
S G Sawant ◽  
C R Geard ◽  
...  
Keyword(s):  
Cell Death ◽  
Colorectal Carcinoma ◽  
Dna Fragmentation ◽  
Carcinoma Cells ◽  
Reproductive Cell ◽  
P53 Status ◽  
Reproductive Cell Death

scholarly journals Momordica charantiaExtract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Chia-Jung Li ◽  
Shih-Fang Tsang ◽  
Chun-Hao Tsai ◽  
Hsin-Yi Tsai ◽  
Jong-Ho Chyuan ◽  
...  
Keyword(s):  
Cell Death ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Dna Fragmentation ◽  
Human Cancer ◽  
Momordica Charantia ◽  
Carcinoma Cells ◽  
Human Cancer Cells ◽  
Adenocarcinoma Cells ◽  
Anti Cancer

Plants are an invaluable source of potential new anti-cancer drugs.Momordica charantiais one of these plants with both edible and medical value and reported to exhibit anticancer activity. To explore the potential effectiveness ofMomordica charantia, methanol extract ofMomordica charantia(MCME) was used to evaluate the cytotoxic activity on four human cancer cell lines, Hone-1 nasopharyngeal carcinoma cells, AGS gastric adenocarcinoma cells, HCT-116 colorectal carcinoma cells, and CL1-0 lung adenocarcinoma cells, in this study. MCME showed cytotoxic activity towards all cancer cells tested, with the approximate IC50ranging from 0.25 to 0.35 mg/mL at 24 h. MCME induced cell death was found to be time-dependent in these cells. Apoptosis was demonstrated by DAPI staining and DNA fragmentation analysis using agarose gel electrophoresis. MCME activated caspase-3 and enhanced the cleavage of downstream DFF45 and PARP, subsequently leading to DNA fragmentation and nuclear condensation. The apoptogenic protein, Bax, was increased, whereas Bcl-2 was decreased after treating for 24 h in all cancer cells, indicating the involvement of mitochondrial pathway in MCME-induced cell death. These findings indicate that MCME has cytotoxic effects on human cancer cells and exhibits promising anti-cancer activity by triggering apoptosis through the regulation of caspases and mitochondria.

scholarly journals Fluorescent magnetic submicronic polymer (FMSP) nanoparticles induce cell death in human colorectal carcinoma cells

2018 ◽  
Vol 46 (sup3) ◽  
pp. S247-S253 ◽  
Author(s):  
Firdos Alam Khan ◽  
Sultan Akhtar ◽  
Dana Almohazey ◽  
Munthar Alomari ◽  
Sarah Ameen Almofty ◽  
...  
Keyword(s):  
Cell Death ◽  
Colorectal Carcinoma ◽  
Carcinoma Cells ◽  
Induce Cell Death ◽  
Human Colorectal Carcinoma

scholarly journals Ionizing Radiation Induces Delayed Hyperrecombination in Mammalian Cells

2004 ◽  
Vol 24 (11) ◽  
pp. 5060-5068 ◽  
Author(s):  
Lei Huang ◽  
Suzanne Grim ◽  
Leslie E. Smith ◽  
Perry M. Kim ◽  
Jac A. Nickoloff ◽  
...  
Keyword(s):  
Cell Death ◽  
Ionizing Radiation ◽  
Genomic Instability ◽  
Mammalian Cells ◽  
Chromosomal Instability ◽  
Radiation Risk ◽  
Green Fluorescence Protein ◽  
Initial Exposure ◽  
Reproductive Cell ◽  
Reproductive Cell Death

ABSTRACT Exposure to ionizing radiation can result in delayed effects that can be detected in the progeny of an irradiated cell multiple generations after the initial exposure. These effects are described under the rubric of radiation-induced genomic instability and encompass multiple genotoxic endpoints. We have developed a green fluorescence protein (GFP)-based assay and demonstrated that ionizing radiation induces genomic instability in human RKO-derived cells and in human hamster hybrid GM10115 cells, manifested as increased homologous recombination (HR). Up to 10% of cells cultured after irradiation produce mixed GFP+/− colonies indicative of delayed HR or, in the case of RKO-derived cells, mutation and deletion. Consistent with prior studies, delayed chromosomal instability correlated with delayed reproductive cell death. In contrast, cells displaying delayed HR showed no evidence of delayed reproductive cell death, and there was no correlation between delayed chromosomal instability and delayed HR, indicating that these forms of genome instability arise by distinct mechanisms. Because delayed hyperrecombination can be induced at doses of ionizing radiation that are not associated with significantly reduced cell viability, these data may have important implications for assessment of radiation risk and understanding the mechanisms of radiation carcinogenesis.

scholarly journals A Caenorhabditis elegans tissue model of radiation-induced reproductive cell death

2006 ◽  
Vol 103 (26) ◽  
pp. 9946-9951 ◽  
Author(s):  
J. B. Weidhaas ◽  
D. M. Eisenmann ◽  
J. M. Holub ◽  
S. V. Nallur
Keyword(s):  
Cell Death ◽  
Caenorhabditis Elegans ◽  
Tissue Model ◽  
Reproductive Cell ◽  
Radiation Induced ◽  
Reproductive Cell Death

scholarly journals Blockade of irradiation-induced autophagosome formation impairs proliferation but does not enhance cell death in HCT-116 human colorectal carcinoma cells

2012 ◽  
Vol 40 (4) ◽  
pp. 1267-1276 ◽  
Author(s):  
ANA CRISTINA DE ALBUQUERQUE-XAVIER ◽  
LILIAN GONÇALVES R. BASTOS ◽  
JULIO CESAR MADUREIRA DE FREITAS ◽  
FERNANDA LEVE ◽  
WALDEMIR FERNÁNDEZ DE SOUZA ◽  
...  
Keyword(s):  
Cell Death ◽  
Colorectal Carcinoma ◽  
Carcinoma Cells ◽  
Autophagosome Formation ◽  
Hct 116 ◽  
Human Colorectal Carcinoma

scholarly journals Vitexin confers HSF-1 mediated autophagic cell death by activating JNK and ApoL1 in colorectal carcinoma cells

Oncotarget ◽  
2017 ◽  
Vol 8 (68) ◽  
pp. 112426-112441 ◽  
Author(s):  
Monika Bhardwaj ◽  
Souren Paul ◽  
Rekha Jakhar ◽  
Imran Khan ◽  
Ji In Kang ◽  
...  
Keyword(s):  
Cell Death ◽  
Colorectal Carcinoma ◽  
Carcinoma Cells ◽  
Autophagic Cell Death
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