In vitro gene transfer in mammalian cells via a new cationic liposome formulation.

1998 ◽  
Author(s):  
M C Kao ◽  
S L Law ◽  
T C Chuang ◽  
Y S Lin
Keyword(s):  
Gene Transfer ◽  
Mammalian Cells ◽  
Cationic Liposome ◽  
Liposome Formulation

Efficacy of cationic liposome-mediated gene transfer to mesangial cells in vitro and in vivo

2001 ◽  
Vol 79 (4) ◽  
pp. 184-189 ◽  
Author(s):  
Henning Madry ◽  
Regina Reszka ◽  
Jürgen Bohlender ◽  
Jürgen Wagner
Keyword(s):  
Gene Transfer ◽  
Mesangial Cells ◽  
Cationic Liposome

Cationic Liposome-Mediated Gene Transfer to Tumor Cells In Vitro and In Vivo

2003 ◽  
pp. 329-338 ◽  
Author(s):  
Kyonghee Son ◽  
Frank Sorgi ◽  
Xiang Gao ◽  
Leaf Huang
Keyword(s):  
Gene Transfer ◽  
Tumor Cells ◽  
Cationic Liposome

scholarly journals The effects of jet nebulisation on cationic liposome-mediated gene transfer in vitro

Gene Therapy ◽  
1998 ◽  
Vol 5 (5) ◽  
pp. 583-593 ◽  
Author(s):  
M Stern ◽  
F Sorgi ◽  
C Hughes ◽  
NJ Caplen ◽  
JE Browning ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Cationic Liposome

In vitro gene transfer to mammalian cells by the use of laser-induced stress waves: effects of stress wave parameters, ambient temperature, and cell type

2006 ◽  
Vol 11 (1) ◽  
pp. 014026 ◽  
Author(s):  
Mitsuhiro Terakawa ◽  
Shunichi Sato ◽  
Hiroshi Ashida ◽  
Kazuya Aizawa ◽  
Maki Uenoyama ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Ambient Temperature ◽  
Stress Wave ◽  
Mammalian Cells ◽  
Stress Waves ◽  
Cell Type ◽  
Wave Parameters ◽  
Induced Stress ◽  
Effects Of Stress

Ultrastructural Changes in Three Different Mammalian Cells Following Ultraviolet Laser Irradiation

1972 ◽  
Vol 30 ◽  
pp. 350-351
Author(s):  
K. Shankar Narayan ◽  
Kailash C. Gupta ◽  
Tohru Okigaki
Keyword(s):  
Ultraviolet Light ◽  
Mammalian Cells ◽  
Biological Effects ◽  
Survival Rates ◽  
Chinese Hamster ◽  
Cell Types ◽  
Differential Sensitivity ◽  
Ultrastructural Changes ◽  
Ultraviolet Laser

The biological effects of short-wave ultraviolet light has generally been described in terms of changes in cell growth or survival rates and production of chromosomal aberrations. Ultrastructural changes following exposure of cells to ultraviolet light, particularly at 265 nm, have not been reported.We have developed a means of irradiating populations of cells grown in vitro to a monochromatic ultraviolet laser beam at a wavelength of 265 nm based on the method of Johnson. The cell types studies were: i) WI-38, a human diploid fibroblast; ii) CMP, a human adenocarcinoma cell line; and iii) Don C-II, a Chinese hamster fibroblast cell strain. The cells were exposed either in situ or in suspension to the ultraviolet laser (UVL) beam. Irradiated cell populations were studied either "immediately" or following growth for 1-8 days after irradiation.Differential sensitivity, as measured by survival rates were observed in the three cell types studied. Pattern of ultrastructural changes were also different in the three cell types.

Vacuoles Induced in Mammalian Cells by Helicobacter Cytotoxin are Acidic Organelles

1990 ◽  
Vol 48 (3) ◽  
pp. 168-169
Author(s):  
M. H. Chestnut ◽  
C. E. Catrenich
Keyword(s):  
Acridine Orange ◽  
Mammalian Cells ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ulcer Disease ◽  
Gastroduodenal Disease ◽  
H Pylori

Helicobacter pylori is a non-invasive, Gram-negative spiral bacterium first identified in 1983, and subsequently implicated in the pathogenesis of gastroduodenal disease including gastritis and peptic ulcer disease. Cytotoxic activity, manifested by intracytoplasmic vacuolation of mammalian cells in vitro, was identified in 55% of H. pylori strains examined. The vacuoles increase in number and size during extended incubation, resulting in vacuolar and cellular degeneration after 24 h to 48 h. Vacuolation of gastric epithelial cells is also observed in vivo during infection by H. pylori. A high molecular weight, heat labile protein is believed to be responsible for vacuolation and to significantly contribute to the development of gastroduodenal disease in humans. The mechanism by which the cytotoxin exerts its effect is unknown, as is the intracellular origin of the vacuolar membrane and contents. Acridine orange is a membrane-permeant weak base that initially accumulates in low-pH compartments. We have used acridine orange accumulation in conjunction with confocal laser scanning microscopy of toxin-treated cells to begin probing the nature and origin of these vacuoles.

Cytological changes induced by platinum-thymine in sarcoma-180 cells: Electron microscopy study

1990 ◽  
Vol 48 (3) ◽  
pp. 290-291
Author(s):  
Gustav Ofosu
Keyword(s):  
Mammalian Cells ◽  
Antitumor Agent ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Limiting Factor ◽  
Sarcoma 180 ◽  
Electron Microscopic ◽  
Cytological Changes

Platinum-thymine has been found to be a potent antitumor agent, which is quite soluble in water, and lack nephrotoxicity as the dose-limiting factor. The drug has been shown to interact with DNA and inhibits DNA, RNA and protein synthesis in mammalian cells in vitro. This investigation was undertaken to elucidate the cytotoxic effects of piatinum-thymine on sarcoma-180 cells in vitro ultrastructurally, Sarcoma-180 tumor bearing mice were treated with intraperitoneal injection of platinum-thymine 40mg/kg. A concentration of 60μg/ml dose of platinum-thymine was used in in vitro experiments. Treatments were at varying time intervals of 3, 7 and 21 days for in vivo experiments, and 30, 60 and 120 min., 6, 12, and 24th in vitro. Controls were not treated with platinum-thymine.Electron microscopic analyses of the treated cells in vivo and in vitro showed drastic cytotoxic effect.

Potential of in vitro pollen maturation for gene transfer

1990 ◽  
Vol 79 (1) ◽  
pp. 194-196
Author(s):  
Anna Alwen ◽  
Norbert Eller ◽  
Monika Kastler ◽  
Rosa Maria Benito Moreno ◽  
Erwin Heberle-Bors
Keyword(s):  
Gene Transfer ◽  
Pollen Maturation
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