scholarly journals Propyl gallate reduces the growth of lung cancer cells through caspase‑dependent apoptosis and G1 phase arrest of the cell cycle

2020 ◽  
Vol 44 (6) ◽  
pp. 2783-2791
Author(s):  
Woo Park
Keyword(s):  
Lung Cancer ◽  
Cell Cycle ◽  
Cancer Cells ◽  
Propyl Gallate ◽  
Lung Cancer Cells ◽  
G1 Phase ◽  
Phase Arrest ◽  
G1 Phase Arrest

Inhibitory Effect of Damulin B from Gynostemma pentaphyllum on Human Lung Cancer Cells

Planta Medica ◽  
2018 ◽  
Vol 85 (05) ◽  
pp. 394-405 ◽  
Author(s):  
Shao-Fang Xing ◽  
Lin-Hua Liu ◽  
Ma-Li Zu ◽  
Man Lin ◽  
Xin-Fang Zhai ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Human Lung ◽  
Inhibitory Effect ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
G1 Phase ◽  
Phase Arrest ◽  
G1 Phase Arrest ◽  
Human Lung Cancer Cells

AbstractDamulin B, a dammarane-type saponin from steamed Gynostemma pentaphyllum, exhibits the strongest activity against human lung carcinoma A549 cells among the isolated active saponins. In this study, the structure-activity relationship of a series of saponin compounds was discussed. The inhibitory effect of damulin B on human lung cancer A549 and H1299 cells was investigated from apoptosis, cell cycle, and migration aspects. In vitro, human lung cancer cells were more susceptible to damulin B treatment than human normal fibroblasts. Damulin B exhibited a strong cytotoxic effect, as evidenced by the increase of apoptosis rate, reduction of mitochondrial membrane potential (MMP), generation of reactive oxygen species, and G0/G1 phase arrest. Furthermore, damulin B activated the following: both intrinsic and extrinsic apoptosis pathways along with early G1 phase arrest via the upregulation of the Bax, Bid, tBid, cleaved caspase-8, and p53 expression levels; downregulation of the procaspase-8/-9, CDK4, CDK6, and cyclin D1 expression levels; and more release of cytochrome c in the cytoplasm. In addition, antimigratory activities and suppressive effects on metastasis-related factors, such as MMP-2 and MMP-9, accompanied by the upregulation of IL-24 were revealed. Altogether, the results proved that damulin B could inhibit human lung cancer cells by inducing apoptosis, blocking the cell cycle at early G0/G1 phase and suppressing the migration. Hence, damulin B has potential therapeutic efficacy against lung cancer.

scholarly journals Lentivirus-mediated knockdown of TSP50 suppresses the growth of non-small cell lung cancer cells via G0/G1 phase arrest

2016 ◽  
Vol 35 (6) ◽  
pp. 3409-3418 ◽  
Author(s):  
WEN-LIANG QIAO ◽  
HAI-YANG HU ◽  
BO-WEN SHI ◽  
LI-JUAN ZANG ◽  
WEI JIN ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cancer Cells ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Lung Cancer Cells ◽  
G1 Phase ◽  
Small Cell Lung ◽  
Phase Arrest ◽  
G1 Phase Arrest

γ-Glutamylcyclotransferase Knockdown Inhibits Growth of Lung Cancer Cells Through G0/G1 Phase Arrest

2015 ◽  
Vol 30 (5) ◽  
pp. 211-216 ◽  
Author(s):  
Zhifeng Lin ◽  
Liwen Xiong ◽  
Jianhua Zhou ◽  
Jin Wang ◽  
Zhao Li ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Lung Cancer Cells ◽  
G1 Phase ◽  
Phase Arrest ◽  
G1 Phase Arrest

Reducing autophagy and inducing G1 phase arrest by aloperine enhances radio-sensitivity in lung cancer cells

2017 ◽  
Author(s):  
Zhijie Xu ◽  
Yuanliang Yan ◽  
Shuangshuang Zeng ◽  
Long Qian ◽  
Shuang Dai ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Lung Cancer Cells ◽  
G1 Phase ◽  
Phase Arrest ◽  
G1 Phase Arrest ◽  
Radio Sensitivity

scholarly journals Anti-proliferation effect of Scutellaria barbata D. Don extract on lung cancer

2021 ◽  
Vol 18 (9) ◽  
pp. 1867-1872
Author(s):  
Zhang Hu ◽  
Duan Jing
Keyword(s):  
Lung Cancer ◽  
Cell Cycle ◽  
Cancer Cells ◽  
A549 Cells ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
Blue Staining ◽  
G1 Phase ◽  
Scutellaria Barbata ◽  
A549 Lung

Purpose: To investigate the effect of Scutellaria barbata D. Don extract (SBDE) on apoptosis and proliferation in A549 human lung cancer cells. Methods: Inverted microscope was used to observe morphological changes in A549 cells after exposure to SBDE. Trypan blue staining of living cells was applied to construct cell growth curve after treatment with varying concentrations of SBDE. The influence of SBDE on cell proliferation, apoptosis and cell cycle was determined by MTT assay while protein expressions of key apoptosis-related enzymes were evaluated by immuno-cytochemical method. Results: SBDE inhibited the growth of A549 lung cancer cells at a concentration range of 20 - 160 μg/mL. Flow cytometry showed that SBDE induced apoptosis in the A549 cells. The proportion of cells in G0/G1-phase increased significantly (p < 0.01), while the proportion of cells in S-phase and G2/Mphase decreased correspondingly, indicating that the cells were in G0/G1-phase arrest. Cell cycle arrest and apoptosis-inducing effect gradually increased with increase in SBDE concentration. With increasing concentrations of SBDE, there were significant increases in the expressions of caspase-3 (p < 0.05), caspase-8 (p < 0.01) and caspase-9 (p < 0.05), and significant decreases in Ki-67 (p < 0.01) and p21 ras protein (p < 0.01). Conclusion: SBDE exerts significant inhibitory effect on the proliferation of A549 lung cancer cells, which can be developed for the treatment of lung cancer patients.

scholarly journals Novel ferrocenyl derivatives exert anti-cancer effect in human lung cancer cells in vitro via inducing G1-phase arrest and senescence

2013 ◽  
Vol 34 (7) ◽  
pp. 960-968 ◽  
Author(s):  
Ying Li ◽  
Han-lin Ma ◽  
Lei Han ◽  
Wei-yong Liu ◽  
Bao-xiang Zhao ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Human Lung ◽  
Human Lung Cancer ◽  
G1 Phase ◽  
Phase Arrest ◽  
G1 Phase Arrest ◽  
Anti Cancer ◽  
Human Lung Cancer Cells

scholarly journals Metformin induces cell cycle arrest at the G1 phase through E2F8 suppression in lung cancer cells

Oncotarget ◽  
2017 ◽  
Vol 8 (60) ◽  
pp. 101509-101519 ◽  
Author(s):  
Dong Hao Jin ◽  
Yujin Kim ◽  
Bo Bin Lee ◽  
Joungho Han ◽  
Hong Kwan Kim ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Lung Cancer Cells ◽  
G1 Phase ◽  
Cycle Arrest
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