scholarly journals Long non‑coding RNA NR2F1‑AS1 facilitates the osteosarcoma cell malignant phenotype via the miR‑485‑5p/miR‑218‑5p/BIRC5 axis

2020 ◽  
Author(s):  
Guanghui Jia ◽  
Yalei Wang ◽  
Yali Yu ◽  
Zijun Li ◽  
Xiangyu Wang
Keyword(s):  
Osteosarcoma Cell ◽  
Malignant Phenotype ◽  
Non Coding Rna ◽  
Long Non Coding Rna

scholarly journals Down-regulation of Long Non-coding RNA TUG1 Inhibits Osteosarcoma Cell Proliferation and Promotes Apoptosis

2013 ◽  
Vol 14 (4) ◽  
pp. 2311-2315 ◽  
Author(s):  
Qiang Zhang ◽  
Pei-Liang Geng ◽  
Pei Yin ◽  
Xiao-Lin Wang ◽  
Jin-Peng Jia ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Osteosarcoma Cell ◽  
Down Regulation ◽  
Non Coding Rna ◽  
Long Non Coding Rna

scholarly journals Long non‑coding RNA LINC00238 suppresses the malignant phenotype of liver cancer by sponging miR‑522

2022 ◽  
Vol 25 (2) ◽  
Author(s):  
Hong-Gang Qian ◽  
Qiong Wu ◽  
Jian-Hui Wu ◽  
Xiu-Yun Tian ◽  
Wei Xu ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Malignant Phenotype ◽  
Non Coding Rna ◽  
Long Non Coding Rna

The role of long non-coding RNA ANRIL in chemosensitivity in osteosarcoma and clinical outcomes.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e22515-e22515
Author(s):  
Asmaa Ferdjallah ◽  
Adam M Lee ◽  
Elise Moore ◽  
Aritro Nath ◽  
R. Stephanie Huang
Keyword(s):  
Cancer Metastasis ◽  
Drug Regimen ◽  
Outcome Analysis ◽  
Osteosarcoma Cell ◽  
Non Coding Rna ◽  
Cellular Sensitivity ◽  
Using Data ◽  
Sirna Knockdown ◽  
Long Non Coding Rna

e22515 Background: Osteosarcoma is a skeletal malignancy affecting children, adolescents, and young adults. Surgical resection and chemotherapy are the mainstay of therapy with a three-drug regimen - cisplatin, doxorubicin, and methotrexate. However, prognosis remains poor mainly due to chemo-resistance and/or metastases. Emerging evidence has shown that long non-coding RNAs (lncRNAs) are implicated in drug sensitivity and cancer metastasis. Our lab has recently identified strong significant association between cellular sensitivity to cisplatin and doxorubicin and expression of a lncRNA ANRIL in a collection of osteosarcoma cell lines. Methods: SiRNA knockdown experiments were carried out in an osteosarcoma cell line, SAOS2. Cells were exposed to multiple concentrations of cisplatin or doxorubicin. Cellular sensitivity to these two drugs was quantified after siRNA experiment with siRNA targeting ANRIL and scramble control at 24, 48 and 72h. Clinical outcome analysis was performed using data from the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) initiative. Results: We successfully knocked down ANRIL expression in SAOS2 cells with 88.5% efficiency. A significant decreased rate of proliferation was detected for cisplatin (7 uM, 72h) in knockdown cells (16.5% +/- 4.1) compared to scramble control (26.4% +/- 0.8). The cisplatin IC50 was decreased in the siRNA knockdown condition as compared to scramble control at 72h (p = 0.02). For doxorubicin, the IC50 was also significantly decreased in the siRNA knockdown condition as compared to scramble control (p = 0.002 and 0.007 at 24h and 72h, respectively). Survival outcomes, metastases at diagnosis, and percent necrosis (an indicator of treatment response) were evaluated in TARGET osteosarcoma patients (n = 121 and n = 43 for OS, percent necrosis, respectively) with varying degrees of ANRIL expression. Above the median ANRIL expression was associated with a greater number of death (p = 0.004) and higher chances of metastases at diagnosis (p = 0.013). Conclusions: Reducing ANRIL expression led to increased cellular sensitivity to cisplatin and doxorubicin, two key treatment agents for osteosarcoma. Higher ANRIL expression was significantly associated with higher rates of osteosarcoma death and metastases at diagnosis in a clinical cohort of patients. These findings may suggest ANRIL serves as a novel biomarker of cisplatin and doxorubicin sensitivity in osteosarcoma.

Long non-coding RNA THOR promotes human osteosarcoma cell growth in vitro and in vivo

2018 ◽  
Vol 499 (4) ◽  
pp. 913-919 ◽  
Author(s):  
Wangzhen Chen ◽  
Meikai Chen ◽  
Yifan Xu ◽  
Xuerong Chen ◽  
Ping Zhou ◽  
...  
Keyword(s):  
Cell Growth ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma ◽  
Non Coding Rna ◽  
Human Osteosarcoma Cell ◽  
Long Non Coding Rna
Sign in / Sign up

Export Citation Format

Share Document