scholarly journals High‑throughput and continuous flow isolation of rare circulating tumor cells and clusters in gastric cancer from human whole blood samples using electromagnetic vibration‑based filtration

2020 ◽  
Author(s):  
An Xiang ◽  
Mei Xue ◽  
Fengling Ren ◽  
Li Wang ◽  
Zichen Ye ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
Whole Blood ◽  
Continuous Flow ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Electromagnetic Vibration ◽  
Whole Blood Samples

scholarly journals Flexible Micro Spring Array Device for High-Throughput Enrichment of Viable Circulating Tumor Cells

2014 ◽  
Vol 60 (2) ◽  
pp. 323-333 ◽  
Author(s):  
Ramdane A Harouaka ◽  
Ming-Da Zhou ◽  
Yin-Ting Yeh ◽  
Waleed J Khan ◽  
Avisnata Das ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood ◽  
Ex Vivo ◽  
Cell Damage ◽  
Flexible Structures ◽  
Capture Efficiency ◽  
Clinical Samples ◽  
Blood Samples ◽  
Whole Blood Samples

Abstract BACKGROUND The dissemination of circulating tumor cells (CTCs) that cause metastases in distant organs accounts for the majority of cancer-related deaths. CTCs have been established as a cancer biomarker of known prognostic value. The enrichment of viable CTCs for ex vivo analysis could further improve cancer diagnosis and guide treatment selection. We designed a new flexible micro spring array (FMSA) device for the enrichment of viable CTCs independent of antigen expression. METHODS Unlike previous microfiltration devices, flexible structures at the micro scale minimize cell damage to preserve viability, while maximizing throughput to allow rapid enrichment directly from whole blood with no need for sample preprocessing. Device performance with respect to capture efficiency, enrichment against leukocytes, viability, and proliferability was characterized. CTCs and CTC microclusters were enriched from clinical samples obtained from breast, lung, and colorectal cancer patients. RESULTS The FMSA device enriched tumor cells with 90% capture efficiency, higher than 104 enrichment, and better than 80% viability from 7.5-mL whole blood samples in <10 min on a 0.5-cm2 device. The FMSA detected at least 1 CTC in 16 out of 21 clinical samples (approximately 76%) compared to 4 out of 18 (approximately 22%) detected with the commercial CellSearch® system. There was no incidence of clogging in over 100 tested fresh whole blood samples. CONCLUSIONS The FMSA device provides a versatile platform capable of viable enrichment and analysis of CTCs from clinically relevant volumes of whole blood.

scholarly journals Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples

2021 ◽  
Vol 2 (1) ◽  
pp. 100311
Author(s):  
Daniella C. Terenzi ◽  
Ehab Bakbak ◽  
Justin Z. Trac ◽  
Mohammad Al-Omran ◽  
Adrian Quan ◽  
...  
Keyword(s):  
Whole Blood ◽  
Progenitor Cell ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Isolation And Characterization ◽  
Whole Blood Samples

The distribution of BrKα/RbKα peak intensity ratio in human whole blood samples

1994 ◽  
Vol 42 (3) ◽  
pp. 231-241 ◽  
Author(s):  
C. Shenberg ◽  
S. Spiegel ◽  
S. Chaitchik ◽  
P. Jordan ◽  
M. Kitzis ◽  
...  
Keyword(s):  
Whole Blood ◽  
Intensity Ratio ◽  
Peak Intensity Ratio ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Peak Intensity ◽  
Whole Blood Samples

Enantioanalysis of tryptophan in whole blood samples using stochastic sensors—A screening test for gastric cancer

Chirality ◽  
2019 ◽  
Vol 32 (2) ◽  
pp. 215-222 ◽  
Author(s):  
Ruxandra‐Maria Ilie‐Mihai ◽  
Raluca‐Ioana Stefan‐van Staden ◽  
Lidia Magerusan ◽  
Maria Coros ◽  
Stela Pruneanu
Keyword(s):  
Gastric Cancer ◽  
Whole Blood ◽  
Screening Test ◽  
Blood Samples ◽  
Whole Blood Samples

Evaluation of an electrochemical biosensor for uric acid measurement in human whole blood samples

2014 ◽  
Vol 436 ◽  
pp. 72-77 ◽  
Author(s):  
Li-Ting Liao ◽  
Chi-Chih Liao ◽  
Chiu-Ching Liu ◽  
Ting-Ya Yang ◽  
Giueng-Chueng Wang
Keyword(s):  
Uric Acid ◽  
Whole Blood ◽  
Electrochemical Biosensor ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Whole Blood Samples

Comparison of an electrochemical biosensor with optical devices for hemoglobin measurement in human whole blood samples

2011 ◽  
Vol 412 (23-24) ◽  
pp. 2150-2156 ◽  
Author(s):  
Ming-Song Hsieh ◽  
Tai-Guang Wu ◽  
Chein-Shyong Su ◽  
Wen-Jing Cheng ◽  
Namik Ozbek ◽  
...  
Keyword(s):  
Whole Blood ◽  
Electrochemical Biosensor ◽  
Optical Devices ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Whole Blood Samples ◽  
Hemoglobin Measurement
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