scholarly journals MicroRNA‑124 negatively regulates chloride intracellular channel�1 to suppress the migration and invasion of liver cancer cells

2019 ◽  
Author(s):  
Xupeng Yue ◽  
Yuanyuan Cui ◽  
Qi You ◽  
Yanxin Lu ◽  
Jufeng Zhang
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Migration And Invasion ◽  
Liver Cancer Cells ◽  
Microrna 124 ◽  
Intracellular Channel

scholarly journals The Down-Regulation of SUZ12 Accelerates the Migration and Invasion of Liver Cancer Cells via Activating ERK1/2 Pathway

2019 ◽  
Vol 10 (6) ◽  
pp. 1375-1384 ◽  
Author(s):  
Cailin Xue ◽  
Kunyuan Wang ◽  
Xiaofeng Jiang ◽  
Chengxin Gu ◽  
Ganxiang Yu ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Migration And Invasion ◽  
Down Regulation ◽  
Liver Cancer Cells

scholarly journals Downexpression of HSD17B6 correlates with clinical prognosis and tumor immune infiltrates in hepatocellular carcinoma

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Lei Lv ◽  
Yujia Zhao ◽  
Qinqin Wei ◽  
Ye Zhao ◽  
Qiyi Yi
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cancer ◽  
Immune Responses ◽  
Cancer Cells ◽  
Negative Regulator ◽  
Functional Enrichment ◽  
Migration And Invasion ◽  
Relative Reduction ◽  
Clinical Prognosis ◽  
Liver Cancer Cells

Abstract Background Hydroxysteroid 17-Beta Dehydrogenase 6 (HSD17B6), a key protein involved in synthetizing dihydrotestosterone, is abundant in the liver. Previous studies have suggested a role for dihydrotestosterone in modulating progress of various malignancies, and HSD17B6 dysfunction was associated with lung cancer and prostate cancer. However, little is known about the detailed role of HSD17B6 in hepatocellular carcinoma (HCC). Methods Clinical implication and survival data related to HSD17B6 expression in patients with HCC were obtained through TCGA, ICGC, ONCOMINE, GEO and HPA databases. Survival analysis plots were drawn with Kaplan–Meier Plotter. The ChIP-seq data were obtained from Cistrome DB. Protein–Protein Interaction and gene functional enrichment analyses were performed in STRING database. The correlations between HSD17B6 and tumor immune infiltrates was investigated via TIMER and xCell. The proliferation, migration and invasion of liver cancer cells transfected with HSD17B6 were evaluated by the CCK8 assay, wound healing test and transwell assay respectively. Expression of HSD17B6, TGFB1 and PD-L1 were assessed by quantitative RT-PCR. Results HSD17B6 expression was lower in HCC compared to normal liver and correlated with tumor stage and grade. Lower expression of HSD17B6 was associated with worse OS, PFS, RFS and DSS in HCC patients. HNF4A bound to enhancer and promoter regions of HSD17B6 gene, activating its transcription, and DNA methylation of HSD17B6 promoter negatively controlled the expression. HSD17B6 and its interaction partners were involved in androgen metabolism and biosynthesis in liver. HSD17B6 inhibited tumor cell proliferation, migration and invasion in liver cancer cells and low expression of HSD17B6 correlated with high immune cells infiltration, relative reduction of immune responses and multiple immune checkpoint genes expression in HCC, probably by regulating the expression of TGFB1. Conclusions This study indicate that HSD17B6 could be a new biomarker for the prognosis of HCC and an important negative regulator of immune responses in HCC.

scholarly journals Roles of CXCL5 on migration and invasion of liver cancer cells

2014 ◽  
Vol 12 (1) ◽  
pp. 193 ◽  
Author(s):  
Xiaojing Xu ◽  
Peixin Huang ◽  
Biwei Yang ◽  
Xiangdong Wang ◽  
Jinglin Xia
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Migration And Invasion ◽  
Liver Cancer Cells

scholarly journals miR-18a-5p Targets FBP1 to Promote Proliferation, Migration, and Invasion of Liver Cancer Cells and Inhibit Cell Apoptosis

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Shan Gao ◽  
Dongjie Zhu ◽  
Jian Zhu ◽  
Lianqiang Shen ◽  
Ming Zhu ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Cell Apoptosis ◽  
Malignant Tumors ◽  
Luciferase Assay ◽  
Migration And Invasion ◽  
Cancer Tissue ◽  
Liver Cancer Cells ◽  
Treatment Plans ◽  
New Treatment

Liver cancer is one of the most aggressive malignant tumors. It is significant to understand the molecular mechanism of liver cancer cells to develop new treatment plans. Studies have identified that FBP1 serves as a cancer inhibitor gene. To research the effect mechanism of FBP1 in liver cancer cells, bioinformatics analysis was performed to study its expression in liver cancer tissue. Survival analysis was also performed. Moreover, starBase database was applied to predict upstream regulatory genes of FBP1. Dual-luciferase assay was performed to testify their targeted relationship. The mRNA and protein expression levels of FBP1 in liver cancer cells were detected by qRT-PCR and western blot, respectively. Cell viability was analyzed by CCK-8 assay. The migratory and invasive abilities of cells were analyzed by Transwell assay. The apoptosis of liver cancer cells was detected by flow cytometry. The results showed that the expression of FBP1 was downregulated in liver cancer tissue and cells. FBP1 low expression was correlated with the poor prognosis of patients. miR-18a-5p could inhibit FBP1 expression. Overexpression of FBP1 could inhibit the progression of liver cancer cells and promote cell apoptosis. Overexpressing miR-18a-5p could promote the progression of liver cancer cells and inhibit cell apoptosis. However, overexpressing FBP1 simultaneously could reverse the effect. miR-18a-5p and FBP1 are expected to be candidates for liver cancer treatment.

scholarly journals MiR-525-3p Enhances the Migration and Invasion of Liver Cancer Cells by Downregulating ZNF395

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e90867 ◽  
Author(s):  
Fei Pang ◽  
Ruopeng Zha ◽  
Yingjun Zhao ◽  
Qifeng Wang ◽  
Di Chen ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Migration And Invasion ◽  
Liver Cancer Cells

The Aqueous Extract of Prunella vulgaris Suppresses Cell Invasion and Migration in Human Liver Cancer Cells by Attenuating Matrix Metalloproteinases

2012 ◽  
Vol 40 (03) ◽  
pp. 643-656 ◽  
Author(s):  
Seung-Hum Kim ◽  
Chung-Yueh Huang ◽  
Cheng-Yu Tsai ◽  
Shu-Yi Lu ◽  
Chien-Chih Chiu ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Aqueous Extract ◽  
Human Liver ◽  
Migration And Invasion ◽  
Liver Carcinoma ◽  
Prunella Vulgaris ◽  
Liver Cancer Cells ◽  
Human Liver Cancer ◽  
Human Liver Cancer Cells

The mechanism of action of Prunella vulgaris L. (PV) affecting cell migration and invasion of human liver cancer cells remains unknown. In this work we showed that the aqueous extract of PV affected migration and invasion of human liver carcinoma cells by inhibiting activities of metalloproteases, MMP-2 and MMP-9, without affecting cell viabilities. We further showed that PV suppressed migration through attenuation of enzymatic activities of MMP-9 and MMP-2 at transcriptional levels and the effects can be correlated with the status of p53 in hepatocarcinoma cells. This work provides a new dimension of understanding on Prunella vulgaris in restraining migration and invasion in human liver cancer cells.

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