Decreased expression of long non-coding RNA GAS5 promotes cell proliferation, migration and invasion, and indicates a poor prognosis in ovarian cancer

Abstract Background X inactivation-specific transcript (XIST) is the long non-coding RNA (lncRNA) related to cancer, which is involved in the development and progression of various types of tumor. However, up to now, the exact role and molecular mechanism of XIST in the progression of ovarian cancer are not clear. We studied the function of XIST in ovarian cancer cells and clinical tumor specimens. Methods RT-qPCR was performed to detect the expression levels of miR-335 and BCL2L2 in ovarian cancer cells and tissues. MTT and transwell assays were carried out to detect cell proliferation, migration, and invasion abilities. Western blot was performed to analyze the expression level of BCL2L2. The interaction between miR-335 and XIST/BCL2L2 was confirmed using a luciferase reporter assay. Results The inhibition of XIST can inhibit the proliferation invasion and migration of human ovarian cancer cells. In addition, the miR-335/BCL2L2 axis was involved in the functions of XIST in ovarian cancer cells. These results suggested that XIST could regulate tumor proliferation and invasion and migration via modulating miR-335/BCL2L2. Conclusion XIST might be a carcinogenic lncRNA in ovarian cancer by regulating miR-335, and it can serve as a therapeutic target in human ovarian cancer.

Download Full-text

Long non-coding RNA FOXD2-AS1 promotes cell proliferation, metastasis and EMT in glioma by sponging miR-506-5p

Open Medicine ◽

10.1515/med-2020-0175 ◽

2020 ◽

Vol 15 (1) ◽

pp. 921-931

Author(s):

Juan Zhao ◽

Xue-Bin Zeng ◽

Hong-Yan Zhang ◽

Jie-Wei Xiang ◽

Yu-Song Liu

Keyword(s):

Cell Proliferation ◽

Epithelial Mesenchymal Transition ◽

Human Cancer ◽

Glioma Cells ◽

Suppressor Gene ◽

Luciferase Reporter ◽

Migration And Invasion ◽

Mesenchymal Transition ◽

Non Coding Rna ◽

Long Non Coding Rna

AbstractLong non-coding RNA forkhead box D2 adjacent opposite strand RNA 1 (FOXD2-AS1) has emerged as a potential oncogene in several tumors. However, its biological function and potential regulatory mechanism in glioma have not been fully investigated to date. In the present study, RT-qPCR was conducted to detect the levels of FOXD2-AS1 and microRNA (miR)-506-5p, and western blot assays were performed to measure the expression of CDK2, cyclinE1, P21, matrix metalloproteinase (MMP)7, MMP9, N-cadherin, E-cadherin and vimentin in glioma cells. A luciferase reporter assay was performed to verify the direct targeting of miR-506-5p by FOXD2-AS1. Subsequently, cell viability was analyzed using the CCK-8 assay. Cell migration and invasion were analyzed using Transwell and wound healing assays, respectively. The results demonstrated that FOXD2-AS1 was significantly overexpressed in glioma cells, particularly in U251 cells. Knockdown of FOXD2-AS1 in glioma cells significantly inhibited cell proliferation, migration, invasion and epithelial–mesenchymal transition (EMT) and regulated the expression of CDK2, cyclinE1, P21, MMP7 and MMP9. Next, a possible mechanism for these results was explored, and it was observed that FOXD2-AS1 binds to and negatively regulates miR-506-5p, which is known to be a tumor-suppressor gene in certain human cancer types. Furthermore, overexpression of miR-506-5p significantly inhibited cell proliferation, migration, invasion and EMT, and these effects could be reversed by transfecting FOXD2-AS1 into the cells. In conclusion, our data suggested that FOXD2-AS1 contributed to glioma proliferation, metastasis and EMT via competitively binding to miR-506-5p. FOXD2-AS1 may be a promising target for therapy in patients with glioma.

Download Full-text

Long non-coding RNA CCAT1 promotes metastasis and poor prognosis in epithelial ovarian cancer

Experimental Cell Research ◽

10.1016/j.yexcr.2017.07.030 ◽

2017 ◽

Vol 359 (1) ◽

pp. 185-194 ◽

Cited By ~ 33

Author(s):

Yuan Cao ◽

Huirong Shi ◽

Fang Ren ◽

Yanyan Jia ◽

Ruitao Zhang

Keyword(s):

Ovarian Cancer ◽

Epithelial Ovarian Cancer ◽

Poor Prognosis ◽

Non Coding Rna ◽

Long Non Coding Rna

Download Full-text

Long non-coding RNA TDRG1 facilitates cell proliferation, migration and invasion in breast cancer via targeting miR-214-5p/CLIC4 axis

Cancer Biology & Therapy ◽

10.1080/15384047.2020.1863120 ◽

2021 ◽

Vol 22 (3) ◽

pp. 248-256

Author(s):

Yanrong Lv ◽

Ke Dong ◽

Haidong Gao

Keyword(s):

Breast Cancer ◽

Cell Proliferation ◽

Migration And Invasion ◽

Non Coding Rna ◽

Long Non Coding Rna

Download Full-text

Overexpression of KIF2A is Suppressed by miR-206 and Associated with Poor Prognosis in Ovarian Cancer

Cellular Physiology and Biochemistry ◽

10.1159/000494467 ◽

2018 ◽

Vol 50 (3) ◽

pp. 810-822 ◽

Cited By ~ 16

Author(s):

Nan Sheng ◽

Yun-Zhao Xu ◽

Qing-Hua Xi ◽

Hai-Yan Jiang ◽

Chen-Yi Wang ◽

...

Keyword(s):

Ovarian Cancer ◽

Cell Proliferation ◽

Cancer Cell ◽

Poor Prognosis ◽

Ovarian Cancer Cell ◽

Expression Profiles ◽

Annexin V ◽

Prognostic Indicator ◽

Luciferase Reporter ◽

Migration And Invasion

Background/Aims: This study aimed to investigate the expression and prognostic value of kinesin family member 2A (KIF2A) and the suppression effects of microRNA-206 (miR-206) on KIF2A in ovarian cancer. Methods: Ovarian cancer tissues from patients and ovarian cancer cell lines (A2780 and SKOV3) were used in this study. miR-206 mimics and control were transiently transfected into cells. RT-qPCR was performed to detect KIF2A mRNA and miR-206 expression levels, Western blot was performed to detect KIF2A protein levels, Dual-Luciferase Reporter Assay was used to examine the inhibition effects of miR-206 on KIF2A mRNA, immunohistochemical staining was used to examine the expression of KIF2A in tissue sections. CCK-8, transwell and Annexin-V-FITC/Propidium Iodide staining with flow cytometry were used to detect the cell proliferation, migration/invasion, and apoptosis respectively. Results: Our study explored the expression profiles of KIF2A and miR-206 in the patients with ovarian cancer. We found that overexpression of KIF2A was associated with a poor prognosis in ovarian cancer. We also found that KIF2A mRNA contains two target sites for miR-206 binding and confirmed that miR-206 directly suppresses KIF2A; inhibits ovarian cancer cell proliferation, migration, and invasion; and induces apoptosis. Conclusion: The results suggest KIF2A could serve a valuable prognostic indicator in ovarian cancer and provide a rationale for treatment of ovarian cancer by targeting KIF2A via miR-206.

Download Full-text