scholarly journals Knockdown of p54nrb inhibits migration, invasion and TNF-α release of human acute monocytic leukemia THP1 cells

2016 ◽  
Vol 35 (6) ◽  
pp. 3742-3748 ◽  
Author(s):  
XIUJUAN ZHANG ◽  
CHANGLI WU ◽  
WEI XIONG ◽  
CHUNLING CHEN ◽  
RONG LI ◽  
...  
Keyword(s):  
Acute Monocytic Leukemia ◽  
Monocytic Leukemia ◽  
Tnf Α

scholarly journals Effect of Essential Oils on the Release of TNF-α and CCL2 by LPS-Stimulated THP‑1 Cells

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 50
Author(s):  
Maria Graça Miguel ◽  
Carina Isabel da Silva ◽  
Luana Farah ◽  
Fernão Castro Braga ◽  
Ana Cristina Figueiredo
Keyword(s):  
Essential Oils ◽  
Inflammatory Diseases ◽  
Thymus Vulgaris ◽  
Acute Monocytic Leukemia ◽  
Monocytic Leukemia ◽  
Foeniculum Vulgare ◽  
Traditional Use ◽  
Eucalyptus Viminalis ◽  
Tnf Α

Plants and their constituents have been used to treat diverse ailments since time immemorial. Many plants are used in diverse external and internal formulations (infusions, alcoholic extracts, essential oils (EOs), etc.) in the treatment of inflammation-associated diseases, such as those affecting the respiratory tract or causing gastrointestinal or joint problems, among others. To support the traditional uses of plant extracts, EOs have been assessed for their alleged anti-inflammatory properties. However, the effect of EOs on the release of cytokines and chemokines has been much less reported. Considering their traditional use and commercial relevance in Portugal and Angola, this study evaluated the effect of EOs on the in vitro inhibition of the cytokine tumor necrosis factor-α (TNF-α) and the chemokine (C-C motif) ligand 2 (CCL2) by lipopolysaccharide (LPS)-stimulated human acute monocytic leukemia cells (THP-1 cells). Twenty EOs extracted from eighteen species from seven families, namely from Amaranthaceae (Dysphania ambrosioides), Apiaceae (Foeniculum vulgare), Asteraceae (Brachylaena huillensis, Solidago virgaurea), Euphorbiaceae (Spirostachys africana), Lamiaceae (Lavandula luisieri, Mentha cervina, Origanum majorana, Satureja montana, Thymbra capitata, Thymus mastichina, Thymus vulgaris, Thymus zygis subsp. zygis), Myrtaceae (Eucalyptus globulus subsp. maidenii, Eucalyptus radiata, Eucalyptus viminalis) and Pinaceae (Pinus pinaster) were assayed for the release of CCL2 and TNF-α by LPS-stimulated THP-1 cells. B. huillensis, S. africana, S. montana, Th. mastichina and Th. vulgaris EOs showed toxicity to THP-1 cells, at the lowest concentration tested (10 μg/mL), using the tetrazolium dye assay. The most active EOs in reducing TNF-α release by LPS-stimulated THP-1 cells were those of T. capitata (51% inhibition at 20 μg/mL) and L. luisieri (15–23% inhibition at 30 μg/mL and 78–83% inhibition at 90 μg/mL). L. luisieri EO induced a concentration-dependent inhibition of CCL2 release by LPS‑stimulated THP-1 cells (23%, 54% and 82% inhibition at 10, 30 and 90 μg/mL, respectively). These EOs are potentially useful in the management of inflammatory diseases mediated by CCL2 and TNF‑α, such as atherosclerosis and arthritis.

A case of acute monocytic leukemia (AMoL or AML‐M5) in an adult FeLV/FIV‐positive cat

2021 ◽  
Vol 50 (1) ◽  
pp. 158-163
Author(s):  
Priscila Beatriz Silva Serpa ◽  
Joanne B. Messick
Keyword(s):  
Acute Monocytic Leukemia ◽  
Monocytic Leukemia

Typical CBFβ/MYH11 fusion due to insertion of the 3′-MYH11 gene into 16q22 in acute monocytic leukemia with normal chromosomes 16 and trisomies 8 and 22

2000 ◽  
Vol 123 (2) ◽  
pp. 137-139 ◽  
Author(s):  
Anna Aventı́n ◽  
Roberta La Starza ◽  
Josep Nomdedéu ◽  
Salut Brunet ◽  
Jorge Sierra ◽  
...  
Keyword(s):  
Acute Monocytic Leukemia ◽  
Monocytic Leukemia

Acute Monocytic Leukemia Presenting as Cutaneous Involvement

Dermatology ◽  
1999 ◽  
Vol 199 (4) ◽  
pp. 349-352 ◽  
Author(s):  
Kazu Nakano ◽  
Hidenari Kusakabe ◽  
Kimihiro Kiyokane
Keyword(s):  
Acute Monocytic Leukemia ◽  
Monocytic Leukemia ◽  
Cutaneous Involvement

scholarly journals Serum Peptidome Based Biomarkers Searching for Monitoring Minimal Residual Disease in Adult Acute Monocytic Leukemia

Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 5233-5233
Author(s):  
Ju Bai ◽  
Yun Yang ◽  
Jianli Wang ◽  
Wanggang Zhang ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Correlation Analysis ◽  
Minimal Residual Disease ◽  
Molecular Diagnosis ◽  
Residual Disease ◽  
Acute Monocytic Leukemia ◽  
Healthy Controls ◽  
Monocytic Leukemia ◽  
Disease States ◽  
Significant Difference ◽  
Minimal Residual

Abstract Background: Acute monocytic leukemia (M5) is one common type in acute myeloid leukemia (AML). The present study found that a few M5 had special reproducible chromosomal abnormalities and molecular abnormalities. The heterogeneity and complexity of M5 lead to lack of specific tumor-associated markers for molecular diagnosis and targeted therapy. CLINPROT system, with unique advantages, is a firenew and distinctive proteomics technology and has been widely used in the researches of solid tumor and hemopathic malignancies. This study was to screen a panel of serum peptides associated with M5 different disease states for molecular diagnosis and monitoring minimal residual disease. Methods: 92 M5 were enrolled for the study from those who were newly diagnosed (ND) in the Second Affiliated Hospital of Xi'an Jiao Tong University from January 2009 to July 2014. The median age was 45 years old (range 18-73) and 57.6% were male. Diagnosis was made according to bone marrow cell morphology, cytochemical staining and cellular immune phenotype. 90 healthy cases (HC) (age range 19-70, median age 43 years old, male/female 51/39) were recruited from those who came to our hospital to undergo healthy physical examination and had no any abnormal symptoms and results. Sera of 92 M5 were gained pre- and post-treatment of chemotherapy. Weak cation exchange magnetic bead combined with matrix assisted laser desorption/ ionization time of flight mass spectrometry were used to compare and analyze serum peptidome of M5 with different disease states. Spearman method was used to do correlation analysis of two variables. Statistical significance was defined as p<0.05. Results: A total of 42 peptides in the molecular weight range of 700-10000 Da were detected using ClinProt system and statistically different between adult M5 and healthy controls. Among them, 9 peptides were elevated and 33 were decreased in M5. Genetic algorithm (GA) was used to obtain a diagnostic model consisting of 6 peptides that could discriminate M5 from controls with a high sensitivity (100%) and specificity (96.67%). Mass charge ratios (m/z) were 6041.91, 4662.15, 7823.02, 9210.97, 1108.91 and 3263.37 respectively. Blind test verified that this model correctly identified 60 cases out of total 62 M5 and 57 cases from 60 healthy controls. The relative intensities of peptides with m/z of 6041.91 and 4662.15 were increased in the ND group and non-complete remission (CR) group, when comparing with the CR group and HC group(p=0.002; p<0.001), but there was no significant difference between the two groups for any of the peptides (p=0.27, p=0.31). No significant difference was found between the CR group and HC group (p=0.22, p=0.41). The relative intensities of peptides with m/z of 7823.02, 9210.97, 1108.91 and 3263.37 were reduced in the ND group and non-CR group when comparing with the CR group and HC group (p<0.001, p=0.0023, p=0.004, p<0.001), and the two groups had no significant difference (p=0.26, p=0.09, p=0.32, p=0.61). No significant difference was observed between the HC group and CR group (p=0.52, p=0.35, p=0.17, p=0.73). Spearman correlation analysis showed that relative intensities of peptides with m/z of 4662.15, 7823.02 and 9210.97 were correlated with high white blood cells (r=0.88, p<0.001; r=-0.89, p<0.001; p=-0.87, p<0.001), FLT3 mutation (r=0.90, p<0.001; r=-0.87, p<0.001; r=-0.88, p<0.001) , extramedullary disease (r=0.80, p<0.001; r=-0.86, p<0.001; r=- 0.82, p<0.001). The relative intensities of the other three peptides had weak correlation with the unfavorable clinical features of M5 at diagnosis. Conclusion: This panel of peptides has encouraging efficiency in discriminating M5 from HCs and potential value in monitoring M5 minimal residual disease. Disclosures No relevant conflicts of interest to declare.

scholarly journals Studies in acute leukemia. I. Antibody-dependent and spontaneous cellular cytotoxicity by leukemic blasts from patients with acute nonlymphoid leukemia

Blood ◽  
1979 ◽  
Vol 54 (3) ◽  
pp. 573-580
Author(s):  
G Fernandes ◽  
T Garrett ◽  
M Nair ◽  
D Straus ◽  
RA Good ◽  
...  
Keyword(s):  
Fc Receptors ◽  
Surface Antigens ◽  
Leukemic Cells ◽  
Acute Monocytic Leukemia ◽  
Cellular Cytotoxicity ◽  
Antibody Dependent Cellular Cytotoxicity ◽  
Adcc Activity ◽  
Monocytic Leukemia ◽  
K562 Cell Line ◽  
Cell Surface Antigens

Leukemic blasts from patients with acute nonlymphoid leukemia were examined for the presence of Ig, receptors for IgGFc, and for their capacity to mediate antibody-dependent cellular cytotoxicity (ADCC) against chicken red blood cells (RBC) coated with IgG and spontaneous cell-mediated cytotoxicity (SCMC) against cells of K562 cell line. Leukemic blasts from acute myeloblastic leukemia (AML) patients lacked both Fc receptors and Ig on their surface, had no SCMC activity and majority, but not all of them, lacked ADCC activity. Leukemic blasts from patients with acute monocytic leukemia (AMOL) had Fc receptors, and 50% had IgG on their surface. IgG was cytophilic and appeared not to be directed against cell-surface antigens. This antibody did not interfere with the ADCC activity of leukemic cells. Leukemic blasts from majority of patients with AMOL mediated ADCC, but had no SCMC activity. An association between ADCC and presence of Fc receptor was observed.

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