scholarly journals Clinical and biological significance of transcription termination factor, RNA polymerase I in human liver hepatocellular carcinoma

2016 ◽  
Vol 35 (4) ◽  
pp. 2073-2080
Author(s):  
HISATERU KOMATSU ◽  
TOMOHIRO IGUCHI ◽  
MASAMI UEDA ◽  
SHO NAMBARA ◽  
TOMOKO SAITO ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Rna Polymerase ◽  
Human Liver ◽  
Transcription Termination ◽  
Biological Significance ◽  
Rna Polymerase I ◽  
Termination Factor ◽  
Transcription Termination Factor ◽  
Polymerase I ◽  
Liver Hepatocellular Carcinoma

scholarly journals Role of the RNA/DNA kinase Grc3 in transcription termination by RNA polymerase I

EMBO Reports ◽  
2010 ◽  
Vol 11 (10) ◽  
pp. 758-764 ◽  
Author(s):  
Priscilla Braglia ◽  
Katrin Heindl ◽  
Alexander Schleiffer ◽  
Javier Martinez ◽  
Nick J Proudfoot
Keyword(s):  
Rna Polymerase ◽  
Transcription Termination ◽  
Rna Polymerase I ◽  
Polymerase I

scholarly journals Nucleolar translocation of human DNA topoisomerase II by ATP depletion and its disruption by the RNA polymerase I inhibitor BMH-21

2021 ◽  
Author(s):  
Keiko Morotomi-Yano ◽  
Ken-ichi Yano
Keyword(s):  
Rna Polymerase ◽  
Topoisomerase Ii ◽  
Biological Significance ◽  
Rna Polymerase I ◽  
Atp Depletion ◽  
Dna Topoisomerase Ii ◽  
Nuclear Dynamics ◽  
Dna Topoisomerase ◽  
The Status ◽  
Polymerase I

DNA topoisomerase II (Top2) is a nuclear protein that resolves DNA topological problems and plays critical roles in multiple nuclear processes. Human cells have two Top2 proteins, Top2A and Top2B, that are localized in both the nucleoplasm and nucleolus. Previously, ATP depletion was shown to augment the nucleolar localization of Top2B, but the molecular details of subnuclear distributions, particularly of Top2A, remained to be fully elucidated in relation to the status of cellular ATP. Here, we analyzed the nuclear dynamics of human Top2A and Top2B in ATP-depleted cells. Both proteins rapidly translocated from the nucleoplasm to the nucleolus in response to ATP depletion. FRAP analysis demonstrated that they were highly mobile in the nucleoplasm and nucleolus. The nucleolar retention of both proteins was sensitive to the RNA polymerase I inhibitor BMH-21, and the Top2 proteins in the nucleolus were immediately dispersed into the nucleoplasm by BMH-21. Under ATP-depleted conditions, the Top2 poison etoposide was less effective, indicating the therapeutic relevance of Top2 subnuclear distributions. These results give novel insights into the subnuclear dynamics of Top2 in relation to cellular ATP levels and also provide discussions about its possible mechanisms and biological significance.

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