scholarly journals Magnetic gold nanoparticle-mediated small interference RNA silencing Bag-1 gene for colon cancer therapy

2015 ◽  
Vol 35 (2) ◽  
pp. 978-984 ◽  
Author(s):  
WENBAI HUANG ◽  
ZHAN'AO LIU ◽  
GUANZHOU ZHOU ◽  
AILING TIAN ◽  
NIANFENG SUN
Keyword(s):  
Colon Cancer ◽  
Cancer Therapy ◽  
Gold Nanoparticle ◽  
Rna Silencing ◽  
Small Interference Rna ◽  
Interference Rna

scholarly journals PIK3C3 Inhibition Promotes Sensitivity to Colon Cancer Therapy by Inhibiting Cancer Stem Cells

Cancers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 2168
Author(s):  
Balawant Kumar ◽  
Rizwan Ahmad ◽  
Swagat Sharma ◽  
Saiprasad Gowrikumar ◽  
Mark Primeaux ◽  
...  
Keyword(s):  
Stem Cells ◽  
Colon Cancer ◽  
Cancer Therapy ◽  
Cancer Stem Cells ◽  
Side Population ◽  
Therapy Resistance ◽  
Fluorescent Reporter ◽  
Combination Treatments ◽  
Gsk 3Β ◽  
Resistance To Chemotherapy

Background: Despite recent advances in therapies, resistance to chemotherapy remains a critical problem in the clinical management of colorectal cancer (CRC). Cancer stem cells (CSCs) play a central role in therapy resistance. Thus, elimination of CSCs is crucial for effective CRC therapy; however, such strategies are limited. Autophagy promotes resistance to cancer therapy; however, whether autophagy protects CSCs to promote resistance to CRC-therapy is not well understood. Moreover, specific and potent autophagy inhibitors are warranted as clinical trials with hydroxychloroquine have not been successful. Methods: Colon cancer cells and tumoroids were used. Fluorescent reporter-based analysis of autophagy flux, spheroid and side population (SP) culture, and qPCR were done. We synthesized 36-077, a potent inhibitor of PIK3C3/VPS34 kinase, to inhibit autophagy. Combination treatments were done using 5-fluorouracil (5-FU) and 36-077. Results: The 5-FU treatment induced autophagy only in a subset of the treated colon cancer. These autophagy-enriched cells also showed increased expression of CSC markers. Co-treatment with 36-077 significantly improved efficacy of the 5-FU treatment. Mechanistic studies revealed that combination therapy inhibited GSK-3β/Wnt/β-catenin signaling to inhibit CSC population. Conclusion: Autophagy promotes resistance to CRC-therapy by specifically promoting GSK-3β/Wnt/β-catenin signaling to promote CSC survival, and 36-077, a PIK3C3/VPS34 inhibitor, helps promote efficacy of CRC therapy.

scholarly journals Identification of Human Kinases Involved in Hepatitis C Virus Replication by Small Interference RNA Library Screening

2007 ◽  
Vol 283 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Lubica Supekova ◽  
Frantisek Supek ◽  
Jongkook Lee ◽  
Shawn Chen ◽  
Nathanael Gray ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Replication ◽  
Library Screening ◽  
Small Interference Rna ◽  
Interference Rna

Reversal of chemoresistance with small interference RNA (siRNA) in etoposide resistant acute myeloid leukemia cells (HL-60)

2015 ◽  
Vol 75 ◽  
pp. 100-104 ◽  
Author(s):  
Saeed Kachalaki ◽  
Behzad Baradaran ◽  
Jafar Majidi ◽  
Mehdi Yousefi ◽  
Dariush Shanehbandi ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Leukemia Cells ◽  
Small Interference Rna ◽  
Acute Myeloid Leukemia Cells ◽  
Interference Rna ◽  
Acute Myeloid

Tat-BMPs-PAMAM Conjugates Enhance Therapeutic Effect of Small Interference RNA on U251 Glioma Cells In Vitro and In Vivo

2010 ◽  
Vol 21 (4) ◽  
pp. 417-426 ◽  
Author(s):  
Lei Han ◽  
Anling Zhang ◽  
Hanjie Wang ◽  
Peiyu Pu ◽  
Xinguo Jiang ◽  
...  
Keyword(s):  
Therapeutic Effect ◽  
Glioma Cells ◽  
Small Interference Rna ◽  
Interference Rna

Inhibition of hepatitis B virus replication by small interference RNA induces expression of MICA in HepG2.2.15 cells

2008 ◽  
Vol 198 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Kai-Fu Tang ◽  
Min Chen ◽  
Jing Xie ◽  
Guan-Bin Song ◽  
Yi-Song Shi ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Virus Replication ◽  
Small Interference Rna ◽  
B Virus ◽  
Interference Rna
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