scholarly journals miRNA expression profile of vulvar squamous cell carcinoma and identification of the oncogenic role of miR-590-5p

2015 ◽  
Vol 35 (1) ◽  
pp. 398-408 ◽  
Author(s):  
XIUHUA YANG ◽  
XIN WU
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Expression Profile ◽  
Squamous Cell ◽  
Mirna Expression ◽  
Mirna Expression Profile ◽  
Vulvar Squamous Cell Carcinoma

scholarly journals SQUAMOUS CELL CARCINOMA

2018 ◽  
Vol 25 (12) ◽  
pp. 1840-1847
Author(s):  
Sabeeh Yousaf ◽  
Sajjad Ali Shahid ◽  
Obaid Hayat
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Expression Profile ◽  
Squamous Cell ◽  
Mirna Expression ◽  
Late Onset ◽  
Mirna Expression Profile ◽  
Benign Tumors ◽  
Tumor Site ◽  
Gender Based

Background: Head and Neck Squamous cell carcinoma is the sixth most common cancer globally with increasing frequency in developing countries. Despite huge advancement in surgery, radiotherapy and chemotherapy there is a little changed in the overall survival rate for patient with HNSCC over the past few decades. Due to its late diagnosis and lack of availability of reliable biomarker for this disease, its incidence is still on rise. Aims &Objectives: This study was aimed to study the expression of miR-21 in the tumor genesis of HNSCC. The objective of the study is to analyze the expression profile of miR-21 in HNSCC, to study the miRNA expression profile of miR-21 between control and tumor samples, to study the expression profile of miR-21 benign tumors and different categories of HNSCC Tumors on the basis of Histological Differentiation, gender-based Comparison of Benign and Malignant HNSCC Tumors, age-based Comparison of Benign and Malignant HNSCC Tumors, tumor Sitebased Comparison of Benign and Malignant HNSCC Tumors. Study Design: Case-control study. Study Setting: The University of Lahore. Period: June -2014 June -2105. Materials & Methods: In this research, 43 Formalin-fixed paraffin embedded (FFPE) tissue samples (31 malignant HNSCC samples and 12 benign tumors from the same region) of both genders and aged 15-80 years were included in this study. 31 cases were malignant tumors were further consisted of 14 well-, 11 moderately- and 6 poorly differentiated tumors. Total RNA was extracted using PureLink FFPE RNA Isolation Kit and Two-Step RT-PCR was performed. TaqMan primer/ probe sets were used for the target miRNA- 221, while RNUB6 was the normalization control.By calculating __Ct and fold change difference according to Livak method. late onset disease the Relative quantification was done to determine the level of expression of miRNA-221. Tumor site did not show any effect on miR-21 expression levels. Results: Our results showed that the malignant samples have higher expression level of miR-21 then benign control samples. Significantly higher expression was observed in moderately and poorly categories of HNSCC. Gender-based expression showed that females had higher level of expression, while it was found that its expression is high in late onset disease. Tumor site did not show any effect on miR-21 expression levels. Conclusion: Our miRNA expression profile provides a potential strategy for finding new head and neck squamous cell carcinoma (HNSCC) molecular targets. miR-21 could be regarded as potential diagnostic marker in HNSCC. 

MiRNA expression profile reveals a prognostic signature for esophageal squamous cell carcinoma

2014 ◽  
Vol 350 (1-2) ◽  
pp. 34-42 ◽  
Author(s):  
Zhaoli Chen ◽  
Jiagen Li ◽  
Liqing Tian ◽  
Chengcheng Zhou ◽  
Yibo Gao ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Esophageal Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Expression Profile ◽  
Squamous Cell ◽  
Mirna Expression ◽  
Mirna Expression Profile ◽  
Prognostic Signature

The role of RhoA in vulvar squamous cell carcinoma: a carcinogenesis, progression, and target therapy marker

Tumor Biology ◽  
2015 ◽  
Vol 37 (3) ◽  
pp. 2879-2890 ◽  
Author(s):  
Jing Wang ◽  
Qiong Wu ◽  
Li-hua Zhang ◽  
Yun-xia Zhao ◽  
Xin Wu
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Target Therapy ◽  
Vulvar Squamous Cell Carcinoma

scholarly journals Role of adjuvant radiation or re-excision for early stage vulvar squamous cell carcinoma with positive or close surgical margins

2019 ◽  
Vol 154 (2) ◽  
pp. 276-279 ◽  
Author(s):  
Sabrina M. Bedell ◽  
Chloe Hedberg ◽  
Anna Griffin ◽  
Hannah Pearson ◽  
Annelise Wilhite ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Early Stage ◽  
Surgical Margins ◽  
Adjuvant Radiation ◽  
Vulvar Squamous Cell Carcinoma

scholarly journals Expression Profile of Circular RNAs in Oral Squamous Cell Carcinoma

2020 ◽  
Vol 10 ◽  
Author(s):  
Yanxiong Shao ◽  
Yuhan Song ◽  
Siming Xu ◽  
Siyi Li ◽  
Haiwen Zhou
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Expression Profile ◽  
Squamous Cell ◽  
Potential Role ◽  
Inhibitory Effect ◽  
Circular Rnas ◽  
Qrt Pcr

BackgroundCircular RNAs (circRNAs) are involved in the pathogenesis of several diseases. Among oral maxillofacial cancers, oral squamous cell carcinoma (OSCC) has the highest incidence. However, the role of circRNAs in OSCC is still not clear. The aim of our study was to evaluate the circRNA expression profile in OSCC and explore further the potential role of circRNAs in the pathogenesis of OSCC.MethodsCircRNA sequencing was performed in 6 pairs of samples of OSCC and normal oral mucosal tissues. Expression of selected circRNAs was validated by qRT-PCR. GO and KEGG analyses were performed and binding relationships between circRNAs and miRNAs were predicted. The hsa_circ_0001766/miR-877-3p/VEGFA axis was selected to further elucidate its role in OSCC.ResultsWe showed that there were 122 differentially expressed (DE) circRNAs. Eight out of 10 selected circRNAs were validated by qRT-PCR. GO and KEGG analyses indicated that the identified DE circRNAs might be involved in the progression of OSCC. Then, after identification by Sanger sequencing and RNase R assay, the upregulated hsa_circ_0001766 was selected to investigate its potential role in OSCC. Bioinformatics analysis showed that hsa_circ_0001766 might act as a competing endogenous RNA (ceRNA) that sponged miR-877-3p to upregulate VEGFA expression. We selected OSCC cell lines SCC9 and SCC25. PCR results showed that the expression of hsa_circ_0001766 and VEGFA was upregulated in SCC9 and SCC25. Subsequently, using western blot, PCR, CCK8, and colony formation assays, we found that knocking down circRNA0001766 inhibited the expression of VEGFA and the proliferation of OSCC cells. Following this, miR-877-3p inhibitor reversed the inhibitory effect of si-hsa_circ_0001766 on expression of VEGFA and proliferation of OSCC cells.ConclusionsIn conclusion, our study revealed the possible role of circRNAs in the pathogenesis of OSCC, and identified the potential role of the hsa_circ_0001766/miR-877-3p/VEGFA axis in OSCC progression.

Sign in / Sign up

Export Citation Format

Share Document