scholarly journals Suppression of LPS-induced epithelial-mesenchymal transition by aqueous extracts of Prunella vulgaris through inhibition of the NF-κB/Snail signaling pathway and regulation of EMT-related protein expression

2015 ◽  
Vol 34 (5) ◽  
pp. 2445-2450 ◽  
Author(s):  
IN-HYE CHO ◽  
EUN HYANG JANG ◽  
DARONG HONG ◽  
BOM JUNG ◽  
MIN-JU PARK ◽  
...  
Keyword(s):  
Protein Expression ◽  
Signaling Pathway ◽  
Epithelial Mesenchymal Transition ◽  
Aqueous Extracts ◽  
Related Protein ◽  
Prunella Vulgaris ◽  
Mesenchymal Transition

Prognostic importance of epithelial-mesenchymal transition-related protein expression in gastric carcinoma

2009 ◽  
Vol 54 (4) ◽  
pp. 442-451 ◽  
Author(s):  
Min A Kim ◽  
Hye Seung Lee ◽  
Hee Eun Lee ◽  
Ji Hun Kim ◽  
Han-Kwang Yang ◽  
...  
Keyword(s):  
Gastric Carcinoma ◽  
Protein Expression ◽  
Epithelial Mesenchymal Transition ◽  
Related Protein ◽  
Mesenchymal Transition ◽  
Prognostic Importance

scholarly journals Prognostic impact of EMT (epithelial-mesenchymal-transition)-related protein expression in endometrial cancer

2013 ◽  
Vol 14 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Yoshimichi Tanaka ◽  
Yoshito Terai ◽  
Hiroshi Kawaguchi ◽  
Satoe Fujiwara ◽  
Saha Yoo ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Protein Expression ◽  
Epithelial Mesenchymal Transition ◽  
Prognostic Impact ◽  
Related Protein ◽  
Mesenchymal Transition

scholarly journals CXCL14 Facilitates the Growth and Metastasis of Ovarian Carcinoma Cells via Activation of the Wnt/β-catenin Signaling Pathway

2021 ◽  
Author(s):  
Li-Na Gao ◽  
Man Hao ◽  
Xiao-Hui Liu ◽  
Li Zhang ◽  
Yan Dong ◽  
...  
Keyword(s):  
Cell Growth ◽  
Protein Expression ◽  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Epithelial Mesenchymal Transition ◽  
Mrna Level ◽  
Metastatic Potential ◽  
Mesenchymal Transition ◽  
Cell Invasiveness

Abstract Background There is an urgent need to identify potential targets in anticancer therapy to improve the survival and prognosis of patients with ovarian cancer (OC). Herein, we investigated the functional significance of chemokine (C-X-C motif) ligand 14 (CXCL14) in OC cell growth and epithelial–mesenchymal transition (EMT).MethodsqRT PCR and western blotting was used to detect CXCL14 mRNA level and protein expression, respectively. The functional mechanism of CXCL14 in OC was investigated by CCK-8, colony formation and transwell assays. The protein expression of CXCL14 and β-catenin in OC tissues was determined by immumohistochemical staining.ResultsWe demonstrated that high levels of CXCL14 were associated with a worse prognosis in patients with OC. CXCL14 knockdown considerably restrained the growth and invasion of OC cells in vitro. In contrast, ectopic CXCL14 overexpression yielded the opposite results. Investigations to determine the underlying molecular mechanisms revealed that the Wnt/β-catenin signaling pathway is involved in CXCL14-facilitated OC cell invasiveness.ConclusionThese data collectively demonstrate that CXCL14 contributes to OC cell growth and metastatic potential by regulating the Wnt/β-catenin signaling pathway.

scholarly journals Involvement of Wnt/β-catenin pathway in the inhibition of invasion and epithelial-mesenchymal transition in ovarian cancer cells

2020 ◽  
Vol 19 (7) ◽  
pp. 1365-1370
Author(s):  
Belikiz Ekem ◽  
Wei Gong ◽  
Lu Han ◽  
Xinmei Wang ◽  
Na Liu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Proliferation ◽  
Cell Migration ◽  
Protein Expression ◽  
Signaling Pathway ◽  
Cell Invasion ◽  
Epithelial Mesenchymal Transition ◽  
Migration And Invasion ◽  
Mesenchymal Transition ◽  
Expression Levels

Purpose: To investigate the effects of zerumbone on cell invasion, epithelial-mesenchymal transition (EMT) and the potential signaling pathway involved in ovarian cancer cells.Methods: Caov-3 cell proliferation was assessed using 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) assay. Wound healing assay was used to determine Caov-3 cell migration while cell invasion was evaluated using Transwell assay. Protein expression was determinedby western blot.Results: Cell viability was reduced by 5, 10, 20, and 50 μM zerumbone (p < 0.05) in a concentrationdependent manner while cell migration and invasion were inhibited by 10 and 20 μM zerumbone (p < 0.05). Protein expression levels of E-cadherin and cytoplasm β-catenin were upregulated by zerumbone (p < 0.05) in a concentration-dependent manner. On the other hand, protein expression levels of Ncadherin, vimentin, ZEB1, nuclear β-catenin, and c-Myc were suppressed by zerumbone (p < 0.05) also in a concentration-dependent manner.Conclusion: The results demonstrate that zerumbone inhibits cell proliferation, migration and invasion, but represses the EMT process via inactivation of Wnt/β-catenin signaling pathway. Keywords: Zerumbone, Ovarian cancer, Wnt/β-catenin pathway, Epithelial-mesenchymal transition

scholarly journals Shenqiwan Ameliorates Renal Fibrosis in Rats by Inhibiting TGF-β1/Smads Signaling Pathway

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Hongshu Chen ◽  
Yiqing Xu ◽  
Yuanxiao Yang ◽  
Xiaojie Zhou ◽  
Shijie Dai ◽  
...  
Keyword(s):  
Protein Expression ◽  
Signaling Pathway ◽  
Renal Fibrosis ◽  
Epithelial Mesenchymal Transition ◽  
Interstitial Fibrosis ◽  
Underlying Mechanism ◽  
Positive Control ◽  
Control Group ◽  
Mesenchymal Transition ◽  
Gene And Protein Expression

Epithelial-mesenchymal transition (EMT) refers to the transition of epithelial cells into mesenchymal cells. Emerging evidence suggests that EMT is a key point in renal interstitial fibrosis (RIF). Traditional Chinese Medicine Shenqiwan (SQW) is widely used in clinical treatment of chronic kidney disease, but the underlying mechanism remains unclear. The purpose of this study is to investigate the effect of SQW on renal fibrosis and its association with TGF-β1/Smads signaling pathway. A rat model of adenine (150 mg/kg) was established and intragastrically treated with various concentrations of SQW at dose of 1.5 g/kg, 3 g/kg, and 6 g/kg. Control group and model group were given the same volume of saline. Meanwhile, the positive control group was treated with Enalapril (4 mg/kg). Animals were sacrificed on 21st day after administration. The results showed that SQW could significantly relieve renal pathological damage caused by adenine, increase gene and protein expression of E-cadherin, and decrease the expression of Vimentin in kidney samples. In addition, SQW efficiently inhibited the mRNA and protein expression of p-Smad2/3 by upregulating Smad7. These results suggest that SQW could slow down the progression of renal fibrosis, possibly by inhibiting TGF-β1/Smads signaling pathway.

scholarly journals Antitumor Effect of Saikosaponin A on Human Neuroblastoma Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Tan Cheng ◽  
Muying Ying
Keyword(s):  
Protein Expression ◽  
Epithelial Mesenchymal Transition ◽  
Neuroblastoma Cells ◽  
Metastatic Tumor ◽  
Inhibitory Effect ◽  
Migration And Invasion ◽  
Related Protein ◽  
Human Neuroblastoma ◽  
Mesenchymal Transition ◽  
Saikosaponin A

Objective. Neuroblastoma (NB) is a highly metastatic tumor in children that develops in the sympathetic nervous system and has a low curative rate. Saikosaponin A (SSA), an active ingredient isolated from the root of Radix Bupleuri, is a natural compound with various pharmacological activities and shows good application prospects in antitumors. This study investigated the antihuman NB activity of SSA and underlying mechanisms associated with its actions. Materials and Methods. The MTT method was used to detect the activity of SSA in inhibiting human NB cell SK-N-AS proliferation. Cell morphology was observed. The flow cytometry technology was used in analyzing the cell apoptosis rate. The Transwell assay evaluated cell migration and invasion following SSA treatment, apoptosis-related protein expression, and angiogenesis-related protein expression, and EMT-related proteins were detected by western blot analysis. Results. SSA showed an inhibitory effect on SK-N-AS cells with the IC50 values of 14.14 μM at 24 h and 12.41 μM at 48 h. Results indicated that SSA has proapoptotic activity, and its proapoptotic activity is positively correlated with the Bax/Bcl-2/caspase-9/caspase-7/PARP pathway. Furthermore, SSA inhibited the invasion and migration of SK-N-AS cells via regulating the angiogenesis-related VEGFR2/Src/Akt pathway and the epithelial-mesenchymal transition- (EMT-) related protein expression. Conclusion. SSA exerts an antihuman NB effect and thus provides foundations for NB treatment.

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