scholarly journals An HCG-rich microenvironment contributes to ovarian cancer cell differentiation into endothelioid cells in a three-dimensional culture system

2015 ◽  
Vol 34 (5) ◽  
pp. 2395-2402 ◽  
Author(s):  
MIN SU ◽  
CHAO FAN ◽  
SAINAN GAO ◽  
AIGUO SHEN ◽  
XIAOYING WANG ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Differentiation ◽  
Cancer Cell ◽  
Culture System ◽  
Ovarian Cancer Cell ◽  
Three Dimensional ◽  
Three Dimensional Culture

scholarly journals Three-Dimensional Cellular Arrangement in Epithelial Ovarian Cancer Cell Lines TOV-21G and SKOV-3 is Associated with Apoptosis-Related miRNA Expression Modulation

2018 ◽  
Vol 11 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Aline Brito de Lima ◽  
Luciana Maria Silva ◽  
Nikole Gontijo Gonçales ◽  
Maria Raquel Santos Carvalho ◽  
Agnaldo Lopes da Silva Filho ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Ovarian Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Mirna Expression ◽  
Ovarian Cancer Cell ◽  
Three Dimensional ◽  
Epithelial Ovarian Cancer Cell ◽  
Ovarian Cancer Cell Lines ◽  
Expression Modulation

scholarly journals The Anti-Proliferative Effect of PI3K/mTOR and ERK Inhibition in Monolayer and Three-Dimensional Ovarian Cancer Cell Models

Cancers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 395
Author(s):  
Elizabeth Dunn ◽  
Kenny Chitcholtan ◽  
Peter Sykes ◽  
Ashley Garrill
Keyword(s):  
Ovarian Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Alternative Pathway ◽  
Three Dimensional ◽  
Cell Aggregates ◽  
Erk Inhibition ◽  
Inhibitor Combination ◽  
Dimensional Cell

Most ovarian cancer patients are diagnosed with advanced stage disease, which becomes unresponsive to chemotherapeutic treatments. The PI3K/AKT/mTOR and the RAS/RAF/MEK/ERK kinase signaling pathways are attractive targets for potential therapeutic inhibitors, due to the high frequency of mutations to PTEN, PIK3CA, KRAS and BRAF in several ovarian cancer subtypes. However, monotherapies targeting one of these pathways have shown modest effects in clinical trials. This limited efficacy of the agents could be due to upregulation and increased signaling via the adjacent alternative pathway. In this study, the efficacy of combined PI3K/mTOR (BEZ235) and ERK inhibition (SCH772984) was investigated in four human ovarian cancer cell lines, grown as monolayer and three-dimensional cell aggregates. The inhibitor combination reduced cellular proliferation in a synergistic manner in OV-90 and OVCAR8 monolayers and in OV-90, OVCAR5 and SKOV3 aggregates. Sensitivity to the inhibitors was reduced in three-dimensional cell aggregates in comparison to monolayers. OV-90 cells cultured in large spheroids were sensitive to the inhibitors and displayed a robust synergistic antiproliferative response to the inhibitor combination. In contrast, OVCAR8 spheroids were resistant to the inhibitors. These findings suggest that combined PI3K/mTOR and ERK inhibition could be a useful strategy for overcoming treatment resistance in ovarian cancer and warrants further preclinical investigation. Additionally, in some cell lines the use of different three-dimensional models can influence cell line sensitivity to PI3K/mTOR and RAS/RAF/MEK/ERK pathway inhibitors.

scholarly journals Inactivation of EGFR/AKT signaling enhances TSA-induced ovarian cancer cell differentiation

2017 ◽  
Vol 37 (5) ◽  
pp. 2891-2896 ◽  
Author(s):  
Genbao Shao ◽  
Wensheng Lai ◽  
Xiaolei Wan ◽  
Jing Xue ◽  
Ye Wei ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Differentiation ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Akt Signaling
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