scholarly journals Meloxicam combined with sorafenib synergistically inhibits tumor growth of human hepatocellular carcinoma cells via ER stress-related apoptosis

2015 ◽  
Vol 34 (4) ◽  
pp. 2142-2150 ◽  
Author(s):  
JINGTAO ZHONG ◽  
PENG XIU ◽  
XIAOFENG DONG ◽  
FUHAI WANG ◽  
HONGLONG WEI ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Tumor Growth ◽  
Er Stress ◽  
Carcinoma Cells ◽  
Human Hepatocellular Carcinoma ◽  
Hepatocellular Carcinoma Cells ◽  
Human Hepatocellular Carcinoma Cells

scholarly journals Excisanin A suppresses proliferation by inhibiting hypoxiainducible factor-1α expression in human hepatocellular carcinoma cells

2021 ◽  
Vol 19 (12) ◽  
pp. 2483-2489
Author(s):  
Li Zhuo Han ◽  
Changgao Jiang ◽  
Chunliu Mi ◽  
Ke Si Wang ◽  
Hong Xiang Zuo ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Protein Synthesis ◽  
Tumor Growth ◽  
Transcriptional Activation ◽  
Carcinoma Cells ◽  
Human Hepatocellular Carcinoma ◽  
Luciferase Reporter ◽  
Mrna Levels ◽  
Hepatocellular Carcinoma Cells ◽  
Human Hepatocellular Carcinoma Cells

Purpose: To investigate the effect of excisanin A on human hepatocellular carcinoma cells as well as to elucidate its mechanism of action. Methods: Molecular docking was used to determine the binding characteristics of excisanin A to HIF-1α protein. The transcriptional activation and viability of excisanin A were assessed using Luciferase reporter and MTT assay. The HIF-1α protein in the nucleus was assayed using western blot and immunofluorescence. HIF-1α and VEGF mRNA levels were evaluated using reverse-transcription polymerase chain reaction (RT-PCR). Cell proliferation was determined by flow cytometry, as well as by EdU and clonogenic assays. In vivo tumor growth was assessed in a murine xenograft model of SKHep1 cells. Results: Excisanin A inhibited HIF-1α transcriptional activation, as well as HIF-1α protein synthesis (p < 0.001). Excisanin A also reduced VEGF protein and mRNA expressions (p < 0.001). In addition, the compound inhibited the proliferation of hepatocellular carcinoma cells. and tumor growth in the xenograft tumor model. Conclusion: Excisanin A is a potent HIF-1α inhibitor, supporting its potential development for human hepatoma therapy. Keywords: Excisanin A, HIF-1α, Protein synthesis, Hepatoma therapy

Sign in / Sign up

Export Citation Format

Share Document