scholarly journals Comparison of 2D- and 3D-culture models as drug-testing platforms in breast cancer

2015 ◽  
Vol 33 (4) ◽  
pp. 1837-1843 ◽  
Author(s):  
YOSHINORI IMAMURA ◽  
TORU MUKOHARA ◽  
YOHEI SHIMONO ◽  
YOHEI FUNAKOSHI ◽  
NAOKO CHAYAHARA ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Testing ◽  
3D Culture ◽  
Culture Models ◽  
2D And 3D

Abstract 319: Comparison of 2D- and 3D-culture models as drug-testing platforms in breast cancer

2015 ◽  
Author(s):  
Yoshinori Imamura ◽  
Toru Mukohara ◽  
Yohei Shimono ◽  
Yohei Funakoshi ◽  
Naoko Chayahara ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Testing ◽  
3D Culture ◽  
Culture Models ◽  
2D And 3D

Effect of red laser irradiation and Ajwain essential oil on 2D and 3D culture models of MDA-MB-231 breast cancer cells

Biologia ◽  
2021 ◽  
Author(s):  
Elnaz Karimi ◽  
Zahra Kianmehr ◽  
Khatereh Khorsandi ◽  
Sepideh Zarie ◽  
Gholamreza Kavoosi
Keyword(s):  
Breast Cancer ◽  
Essential Oil ◽  
Cancer Cells ◽  
Laser Irradiation ◽  
Breast Cancer Cells ◽  
3D Culture ◽  
Culture Models ◽  
2D And 3D

Length vs. stiffness: which plays a dominant role in the cellular uptake of fructose-based rod-like micelles by breast cancer cells in 2D and 3D cell culture models?

2018 ◽  
Vol 6 (25) ◽  
pp. 4223-4231 ◽  
Author(s):  
Jiacheng Zhao ◽  
Hongxu Lu ◽  
Yin Yao ◽  
Sylvia Ganda ◽  
Martina H. Stenzel
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Dominant Role ◽  
3D Cell Culture ◽  
Culture Models ◽  
Nano Rods ◽  
2D And 3D ◽  
Cell Culture Models

Internalization of rod-like micelles by breast cancer cells is significantly affected by the stiffness of nano-rods.

scholarly journals Pre-Clinical Drug Testing in 2D and 3D Human In Vitro Models of Glioblastoma Incorporating Non-Neoplastic Astrocytes: Tunneling Nano Tubules and Mitochondrial Transfer Modulates Cell Behavior and Therapeutic Response

2019 ◽  
Vol 20 (23) ◽  
pp. 6017 ◽  
Author(s):  
Prospero Civita ◽  
Diana M. Leite ◽  
Geoffrey Pilkington
Keyword(s):  
Drug Resistance ◽  
Hyaluronic Acid ◽  
Drug Testing ◽  
Tunneling Nanotubes ◽  
Mitochondrial Transfer ◽  
Culture Models ◽  
And Migration ◽  
2D And 3D

The role of astrocytes in the glioblastoma (GBM) microenvironment is poorly understood; particularly with regard to cell invasion and drug resistance. To assess this role of astrocytes in GBMs we established an all human 2D co-culture model and a 3D hyaluronic acid-gelatin based hydrogel model (HyStem™-HP) with different ratios of GBM cells to astrocytes. A contact co-culture of fluorescently labelled GBM cells and astrocytes showed that the latter promotes tumour growth and migration of GBM cells. Notably, the presence of non-neoplastic astrocytes in direct contact, even in low amounts in co-culture, elicited drug resistance in GBM. Recent studies showed that non-neoplastic cells can transfer mitochondria along tunneling nanotubes (TNT) and rescue damaged target cancer cells. In these studies, we explored TNT formation and mitochondrial transfer using 2D and 3D in vitro co-culture models of GBM and astrocytes. TNT formation occurs in glial fibrillary acidic protein (GFAP) positive “reactive” astrocytes after 48 h co-culture and the increase of TNT formations was greater in 3D hyaluronic acid-gelatin based hydrogel models. This study shows that human astrocytes in the tumour microenvironment, both in 2D and 3D in vitro co-culture models, could form TNT connections with GBM cells. We postulate that the association on TNT delivery non-neoplastic mitochondria via a TNT connection may be related to GBM drug response as well as proliferation and migration.

scholarly journals Cellular effects of paclitaxel-loaded iron oxide nanoparticles on breast cancer using different 2D and 3D cell culture models

2018 ◽  
Vol Volume 14 ◽  
pp. 161-180 ◽  
Author(s):  
Stephan Lugert ◽  
Harald Unterweger ◽  
Marina Mühlberger ◽  
Christina Janko ◽  
Sebastian Draack ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
3D Cell Culture ◽  
Oxide Nanoparticles ◽  
Cellular Effects ◽  
Culture Models ◽  
2D And 3D ◽  
Cell Culture Models

Abstract 141: Influence of the Collagen Processing Heat Shock Protein 47 on Cardiomyocyte Homeostasis and Maturation

2014 ◽  
Vol 115 (suppl_1) ◽  
Author(s):  
Sumon Sur ◽  
Peter Christalla ◽  
Angelica Roa ◽  
Wolfram H Zimmermann
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Cardiac Tissue ◽  
Isometric Force ◽  
Embryonic Stem ◽  
3D Culture ◽  
Heat Shock Protein 47 ◽  
Culture Models ◽  
2D And 3D

PURPOSE: Heat shock protein 47 (Hsp47) is a collagen-specific molecular chaperone required for maturation of collagen type 1. Little is known about the role of Hsp47 in the heart. This study aims to investigate whether Hsp47 is important for cardiomyocyte maturation and assembly into functional syncytia. METHODS: We made use of recently developed 2D and 3D culture platforms to scrutinize the role of Hsp47 for cardiomyocyte maturation and function, i.e., (1) 2D model: culture of embryonic stem cell-derived cardiomyocytes (ESC-CM) on ECMs derived from both mouse embryonic wild type (WT)- and Hsp47 (-/-) fibroblasts and (2) 3D model: construction of engineered heart muscle (EHM) consisting of purified ESC-CMs with WT- or Hsp47 (-/-) fibroblasts. Cultures were investigated using confocal microscopy and isometric force measurements. RESULTS: ESC-CMs showed mainly immature polygonal shaped morphology and disorganized sarcomeric structures when cultured on ECM secreted by Hsp47 (-/-) fibroblasts; this morphology was in clear contrast to the mostly anisotropic cardiomyocyte appearance in control conditions. EHM with Hsp47 (-/-) fibroblasts did not develop measurable contractile forces. In contrast, WT fibroblast-supplemented EHMs contracted regularly (WT: 0.14±0.1 mN at 2.4 mM Ca2+; n=8/group). Histological analysis of Hsp47 (-/-) EHMs showed mainly rounded and underdeveloped cardiomyocytes comparable to the cardiomyocyte phenotype observed in EHMs containing only ESC-CMs, without fibroblasts. CONCLUSIONS: Hsp47 influences the deposition of ECM-collagen and affects cardiomyocyte morphology and functionality in our 2D and 3D culture models. Our data collectively suggest that Hsp47 may be an attractive target for the regulation of cardiac tissue homeostasis.

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