scholarly journals Inhibitory effects of the recombinant human serine protease inhibitor Hespintor on the proliferation, migration and invasion of hepatocellular carcinoma cells

2014 ◽  
Vol 33 (2) ◽  
pp. 729-736 ◽  
Author(s):  
XUE-LEI WANG ◽  
YUE LI ◽  
XIAO-YAN CHENG ◽  
QING CHI ◽  
XIAO-KUN YAO ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Protease Inhibitor ◽  
Serine Protease ◽  
Serine Protease Inhibitor ◽  
Carcinoma Cells ◽  
Migration And Invasion ◽  
Inhibitory Effects ◽  
Hepatocellular Carcinoma Cells

scholarly journals Tazarotene-Induced Gene 1 (TIG1) Interacts with Serine Protease Inhibitor Kazal-Type 2 (SPINK2) to Inhibit Cellular Invasion of Testicular Carcinoma Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Rong-Yaun Shyu ◽  
Chun-Hua Wang ◽  
Chang-Chieh Wu ◽  
Lu-Kai Wang ◽  
Mao-Liang Chen ◽  
...  
Keyword(s):  
Protease Inhibitor ◽  
Testicular Cancer ◽  
Serine Protease ◽  
Epithelial Mesenchymal Transition ◽  
Serine Protease Inhibitor ◽  
Carcinoma Cells ◽  
Migration And Invasion ◽  
Mesenchymal Transition ◽  
Testicular Carcinoma ◽  
Cancer Tissues

Tazarotene-induced gene 1 (TIG1) encodes a protein that is a retinoid-regulated tumor suppressor. TIG1 is expressed in most normal tissues, and downregulation of TIG1 expression in multiple cancers is caused by promoter hypermethylation. Kazal-type serine protease inhibitor-2 (SPINK2) is a serine protease inhibitor, and the SPINK protein family has been shown to inhibit the expression of urokinase-type plasminogen activator (uPA). In addition, increased levels of uPA and the uPA receptor were observed in testicular cancer tissues. This study demonstrated that TIG1 interacts with SPINK2 in NT2/D1 testicular carcinoma cells. TIG1 and SPINK2 were highly expressed in normal testis tissues, while low expression levels of TIG1 and SPINK2 were found in testicular cancer tissues. TIG1 inhibited cell invasion, migration, and epithelial–mesenchymal transition (EMT) of NT2/D1 cells. SPINK2 enhanced TIG1-regulated uPA activity and EMT suppression, while silencing SPINK2 alleviated TIG1-mediated EMT regulation, cell migration, and invasion. Therefore, the results suggest that the interaction between TIG1 and SPINK2 plays an important role in the inhibition of testicular cancer cell EMT, and suppression is mediated through downregulation of the uPA/uPAR signaling pathway.

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