scholarly journals Proteomic analysis reveals that MAEL, a component of nuage, interacts with stress granule proteins in cancer cells

2013 ◽  
Vol 31 (1) ◽  
pp. 342-350 ◽  
Author(s):  
LIQIN YUAN ◽  
YUZHONG XIAO ◽  
QIUZHI ZHOU ◽  
DONGMEI YUAN ◽  
BAIPING WU ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Proteomic Analysis ◽  
Stress Granule ◽  
Granule Proteins

scholarly journals Targeting AKT/mTOR and Bcl-2 for Autophagic and Apoptosis Cell Death in Lung Cancer: Novel Activity of a Polyphenol Compound

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 534
Author(s):  
Sucharat Tungsukruthai ◽  
Onrapak Reamtong ◽  
Sittiruk Roytrakul ◽  
Suchada Sukrong ◽  
Chanida Vinayanwattikun ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Death ◽  
Cancer Cells ◽  
Proteomic Analysis ◽  
Interaction Analysis ◽  
Time Dependent ◽  
The Novel ◽  
Protein Protein Interaction ◽  
Autophagy Induction ◽  
Acidic Vesicle

Autophagic cell death (ACD) is an alternative death mechanism in resistant malignant cancer cells. In this study, we demonstrated how polyphenol stilbene compound PE5 exhibits potent ACD-promoting activity in lung cancer cells that may offer an opportunity for novel cancer treatment. Cell death caused by PE5 was found to be concomitant with dramatic autophagy induction, as indicated by acidic vesicle staining, autophagosome, and the LC3 conversion. We further confirmed that the main death induction caused by PE5 was via ACD, since the co-treatment with an autophagy inhibitor could reverse PE5-mediated cell death. Furthermore, the defined mechanism of action and upstream regulatory signals were identified using proteomic analysis. Time-dependent proteomic analysis showed that PE5 affected 2142 and 1996 proteins after 12 and 24 h of treatment, respectively. The crosstalk network comprising 128 proteins that control apoptosis and 25 proteins involved in autophagy was identified. Protein–protein interaction analysis further indicated that the induction of ACD was via AKT/mTOR and Bcl-2 suppression. Western blot analysis confirmed that the active forms of AKT, mTOR, and Bcl-2 were decreased in PE5-treated cells. Taken together, we demonstrated the novel mechanism of PE5 in shifting autophagy toward cell death induction by targeting AKT/mTOR and Bcl-2 suppression.

Proteomic analysis of the E2F1 response in p53-negative cancer cells: New aspects in the regulation of cell survival and death

PROTEOMICS ◽  
2006 ◽  
Vol 6 (21) ◽  
pp. 5735-5745 ◽  
Author(s):  
Zhenpeng Li ◽  
Michael Kreutzer ◽  
Stefan Mikkat ◽  
Nikica Miše ◽  
Michael O. Glocker ◽  
...  
Keyword(s):  
Cell Survival ◽  
Cancer Cells ◽  
Proteomic Analysis

scholarly journals Small molecules for modulating protein driven liquid-liquid phase separation in treating neurodegenerative disease

2019 ◽  
Author(s):  
Richard J. Wheeler ◽  
Hyun O. Lee ◽  
Ina Poser ◽  
Arun Pal ◽  
Thom Doeleman ◽  
...  
Keyword(s):  
Phase Separation ◽  
Neurodegenerative Disease ◽  
Stress Granules ◽  
Life Time ◽  
Stress Granule ◽  
Phase Behaviour ◽  
Granule Proteins ◽  
Lateral Sclerosis ◽  
Liquid Liquid Phase Separation

AbstractAmyotrophic lateral sclerosis (ALS) is a neurodegenerative disease with few avenues for treatment. Many proteins implicated in ALS associate with stress granules, which are examples of liquid-like compartments formed by phase separation. Aberrant phase transition of stress granules has been implicated in disease, suggesting that modulation of phase transitions could be a possible therapeutic route. Here, we combine cell-based and protein-based screens to show that lipoamide, and its related compound lipoic acid, reduce the propensity of stress granule proteins to aggregate in vitro. More significantly, they also prevented aggregation of proteins over the life time of Caenorhabditis elegans. Observations that they prevent dieback of ALS patient-derived (FUS mutant) motor neuron axons in culture and recover motor defects in Drosophila melanogaster expressing FUS mutants suggest plausibility as effective therapeutics. Our results suggest that altering phase behaviour of stress granule proteins in the cytoplasm could be a novel route to treat ALS.

scholarly journals Proteomic analysis of zoledronic-acid resistant prostate cancer cells unveils novel pathways characterizing an invasive phenotype

Oncotarget ◽  
2014 ◽  
Vol 6 (7) ◽  
pp. 5324-5341 ◽  
Author(s):  
Maria Rita Milone ◽  
Biagio Pucci ◽  
Katia Bifulco ◽  
Federica Iannelli ◽  
Rita Lombardi ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Zoledronic Acid ◽  
Cancer Cells ◽  
Proteomic Analysis ◽  
Prostate Cancer Cells ◽  
Invasive Phenotype

scholarly journals Integrated Proteomic Analysis of Human Cancer Cells and Plasma from Tumor Bearing Mice for Ovarian Cancer Biomarker Discovery

PLoS ONE ◽  
2009 ◽  
Vol 4 (11) ◽  
pp. e7916 ◽  
Author(s):  
Sharon J. Pitteri ◽  
Lellean JeBailey ◽  
Vitor M. Faça ◽  
Jason D. Thorpe ◽  
Melissa A. Silva ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Proteomic Analysis ◽  
Biomarker Discovery ◽  
Human Cancer ◽  
Cancer Biomarker ◽  
Human Cancer Cells ◽  
Tumor Bearing

Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells

2018 ◽  
Vol 160 ◽  
pp. 344-350 ◽  
Author(s):  
Hui-I Yu ◽  
Hsiu-Chuan Chou ◽  
Yi-Ching Su ◽  
Li-Hsun Lin ◽  
Chieh-Hsiang Lu ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Cancer Cells ◽  
Proteomic Analysis ◽  
Thyroid Cancer Cells

Nanoscale Analysis Reveals the Maturation of Neurodegeneration-Associated Protein Aggregates: Grown in mRNA Granules then Released by Stress Granule Proteins

ACS Nano ◽  
2017 ◽  
Vol 11 (7) ◽  
pp. 7189-7200 ◽  
Author(s):  
Sanae Abrakhi ◽  
Dmitry A. Kretov ◽  
Bénédicte Desforges ◽  
Ioana Dobra ◽  
Ahmed Bouhss ◽  
...  
Keyword(s):  
Protein Aggregates ◽  
Stress Granule ◽  
Mrna Granules ◽  
Granule Proteins

A proteomic analysis of differential cellular responses to the short-chain fatty acids butyrate, valerate and propionate in colon epithelial cancer cells

2012 ◽  
Vol 8 (4) ◽  
pp. 1146-1156 ◽  
Author(s):  
Josephine Kilner ◽  
Jennifer S. Waby ◽  
Joanna Chowdry ◽  
Abdul Q. Khan ◽  
Josselin Noirel ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Cancer Cells ◽  
Proteomic Analysis ◽  
Cellular Responses ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Epithelial Cancer ◽  
Chain Fatty Acids
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