scholarly journals Promoter methylation status of the FHIT gene and Fhit expression: Association with HER2/neu status in breast cancer patients

2013 ◽  
Vol 30 (5) ◽  
pp. 2270-2278 ◽  
Author(s):  
YOUNG JU JEONG ◽  
HYE YEON JEONG ◽  
SANG MIN LEE ◽  
JIN GU BONG ◽  
SUNG HWAN PARK ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Promoter Methylation ◽  
Methylation Status ◽  
Breast Cancer Patients ◽  
Fhit Gene ◽  
Promoter Methylation Status

scholarly journals Epigenetic Alteration and its Association With Downregulated FOXP3 Gene in Indian Breast Cancer Patients

2021 ◽  
Vol 12 ◽  
Author(s):  
Sadaf ◽  
Naseem Akhter ◽  
Raed A. Alharbi ◽  
Abdulmajid A. A. Sindi ◽  
Mohammad Zeeshan Najm ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Protein Expression ◽  
Cancer Patients ◽  
Promoter Methylation ◽  
Histological Grade ◽  
Mrna Level ◽  
Methylation Status ◽  
Positive Lymph Node ◽  
Breast Cancer Patients ◽  
Protein Loss

Background:FOXP3 gene, known to be a potential tumor suppressor, has been identified to interact with HER2 in mammary cancer. Moreover, the high expression of FOXP3 serves as a good predictor of the survival of patients in breast cancer, prostate cancer, and gastric cancer. The expression and epigenetic alterations were evaluated in female breast cancer patients.Material and Methods: Expression studies at the mRNA level and protein level were conducted in 140 breast cancer cases by real-time PCR and immunohistochemistry, respectively. Epigenetic studies were also conducted by analyzing the methylation status at the promoter region of the gene using MS-PCR.Results:FOXP3 mRNA expression and protein expression were downregulated in breast cancer patients. The absence of FOXP3 protein expression is significantly associated with promoter methylation, where 70 methylated cases exhibited protein loss (70/95, 73.6%). Statistically, we also found a significant correlation between FOXP3 protein expression and TNM stage, promoter methylation, and histological grade. The methylated FOXP3 cases that did not express protein were also significantly associated with positive lymph node metastasis and HER-2 status.Conclusion: The expression profile of FOXP3 may serve as a prognostic factor. In short, FOXP3 may stand in the most crucial list of biomarkers for breast cancer, bringing compelling results in terms of treatment and management of the disease.

scholarly journals LCN2 Promoter Methylation Status as Novel Predictive Marker for Microvessel Density and Aggressive Tumor Phenotype in Breast Cancer Patients

2015 ◽  
Vol 16 (12) ◽  
pp. 4965-4969 ◽  
Author(s):  
Phanni bhushann Meka ◽  
Sarika Jarjapu ◽  
Santhoshi Rani Nanchari ◽  
Sandeep Kumar Vishwakarma ◽  
Prajitha Mohandas Edathara ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Microvessel Density ◽  
Promoter Methylation ◽  
Methylation Status ◽  
Predictive Marker ◽  
Breast Cancer Patients ◽  
Tumor Phenotype ◽  
Aggressive Tumor ◽  
Aggressive Tumor Phenotype

Clinical significance of Stratifin, ERα and PR promoter methylation in tumor and serum DNA in Indian breast cancer patients

2010 ◽  
Vol 43 (4-5) ◽  
pp. 380-386 ◽  
Author(s):  
Sameer Mirza ◽  
Gayatri Sharma ◽  
Rajinder Parshad ◽  
Anurag Srivastava ◽  
Siddartha Datta Gupta ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Clinical Significance ◽  
Promoter Methylation ◽  
Breast Cancer Patients ◽  
Serum Dna

scholarly journals Clinical impact of PTEN methylation status as a prognostic marker for breast cancer

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Amal Ramadan ◽  
Maha Hashim ◽  
Amr Abouzid ◽  
Menha Swellam
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Tumor Markers ◽  
Prognostic Marker ◽  
Methylation Status ◽  
Clinical Impact ◽  
Breast Cancer Patients ◽  
Duct Carcinoma ◽  
Cancer Group ◽  
And Control

Abstract Background Aberrant DNA methylation of phosphatase and tensin homolog (PTEN) gene has been found in many cancers. The object of this study was to evaluate the clinical impact of PTEN methylation as a prognostic marker in breast cancer. The study includes 153 newly diagnosed females, and they were divided according to their clinical diagnosis into breast cancer patients (n = 112) and females with benign breast lesion (n = 41). A group of healthy individuals (n = 25) were recruited as control individuals. Breast cancer patients were categorized into early stage (0–I, n = 48) and late stage (II–III, n = 64), and graded into low grade (I–II, n = 42) and high grade (III, n = 70). Their pathological types were invasive duct carcinoma (IDC) (n = 66) and duct carcinoma in situ (DCI) (n = 46). Tumor markers (CEA and CA15.3) were detected using ELISA. DNA was taken away from the blood, and the PTEN promoter methylation level was evaluated using the EpiTect Methyl II PCR method. Results The findings revealed the superiority of PTEN methylation status as a good discriminator of the cancer group from the other two groups (benign and control) with its highest AUC and increased sensitivity (96.4%) and specificity (100%) over tumor markers (50% and 84% for CEA and 49.1% and 86.4% for CA15.3), respectively. The frequency of PTEN methylation was 96.4% of breast cancer patients and none of the benign and controls showed PTEN methylation and the means of PTEN methylation (87 ± 0.6) were significantly increased in blood samples of breast cancer group as compared to both benign and control groups (25 ± 0.7 and 12.6 ± 0.3), respectively. Methylation levels of PTEN were higher in the blood of patients with ER-positive than in patients with ER-negative cancers (P = 0.007) and in HER2 positive vs. HER2 negative tumors (P = 0.001). The Kaplan-Meier analysis recognizes PTEN methylation status as a significant forecaster of bad progression-free survival (PFS) and overall survival (OS), after 40 months follow-up. Conclusions PETN methylation could be supposed as one of the epigenetic aspects influencing the breast cancer prognosis that might foretell more aggressive actions and worse results in breast cancer patients.

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