scholarly journals Isolation and characterization of biliary epithelial and stromal cells from resected human cholangiocarcinoma: A novel in vitro model to study tumor-stroma interactions

2013 ◽  
Vol 30 (3) ◽  
pp. 1143-1148 ◽  
Author(s):  
MARCO MASSANI ◽  
TOMMASO STECCA ◽  
LUCA FABRIS ◽  
EZIO CARATOZZOLO ◽  
CESARE RUFFOLO ◽  
...  
Keyword(s):  
Stromal Cells ◽  
In Vitro Model ◽  
Tumor Stroma ◽  
Isolation And Characterization ◽  
Vitro Model

scholarly journals Extracellular Vesicles Are More Potent Than Adipose Mesenchymal Stromal Cells to Exert an Anti-Fibrotic Effect in an In Vitro Model of Systemic Sclerosis

2021 ◽  
Vol 22 (13) ◽  
pp. 6837
Author(s):  
Pauline Rozier ◽  
Marie Maumus ◽  
Claire Bony ◽  
Alexandre Thibault Jacques Maria ◽  
Florence Sabatier ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Extracellular Vesicles ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
In Vitro Model ◽  
Specific Treatment ◽  
Complex Disorder ◽  
Molecular Features ◽  
Vitro Model

Systemic sclerosis (SSc) is a complex disorder resulting from dysregulated interactions between the three main pathophysiological axes: fibrosis, immune dysfunction, and vasculopathy, with no specific treatment available to date. Adipose tissue-derived mesenchymal stromal cells (ASCs) and their extracellular vesicles (EVs) have proved efficacy in pre-clinical murine models of SSc. However, their precise action mechanism is still not fully understood. Because of the lack of availability of fibroblasts isolated from SSc patients (SSc-Fb), our aim was to determine whether a TGFβ1-induced model of human myofibroblasts (Tβ-Fb) could reproduce the characteristics of SSc-Fb and be used to evaluate the anti-fibrotic function of ASCs and their EVs. We found out that Tβ-Fb displayed the main morphological and molecular features of SSc-Fb, including the enlarged hypertrophic morphology and expression of several markers associated with the myofibroblastic phenotype. Using this model, we showed that ASCs were able to regulate the expression of most myofibroblastic markers on Tβ-Fb and SSc-Fb, but only when pre-stimulated with TGFβ1. Of interest, ASC-derived EVs were more effective than parental cells for improving the myofibroblastic phenotype. In conclusion, we provided evidence that Tβ-Fb are a relevant model to mimic the main characteristics of SSc fibroblasts and investigate the mechanism of action of ASCs. We further reported that ASC-EVs are more effective than parental cells suggesting that the TGFβ1-induced pro-fibrotic environment may alter the function of ASCs.

scholarly journals Establishment and Characterization of an In Vitro Model of Fas-Mediated Hepatocyte Cell Death

2014 ◽  
pp. 95-103
Author(s):  
Mathieu Vinken ◽  
Michaël Maes ◽  
Sara Crespo Yanguas ◽  
Joost Willebrords ◽  
Tamara Vanhaecke ◽  
...  
Keyword(s):  
Cell Death ◽  
In Vitro Model ◽  
Vitro Model

Characterization of chaotropic effects of detergents on lipids homeostasis in an in vitro model of ocular toxicity: a molecular network based-lipidomic approach

2021 ◽  
Vol 350 ◽  
pp. S129-S130
Author(s):  
R Magny ◽  
K. Kessal ◽  
A. Regazzetti ◽  
O. Laprévote ◽  
C. Baudouin ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Molecular Network ◽  
Ocular Toxicity ◽  
Vitro Model

Exosome derived from murine adipose-derived stromal cells: Neuroprotective effect on in vitro model of amyotrophic lateral sclerosis

2016 ◽  
Vol 340 (1) ◽  
pp. 150-158 ◽  
Author(s):  
Roberta Bonafede ◽  
Ilaria Scambi ◽  
Daniele Peroni ◽  
Valentina Potrich ◽  
Federico Boschi ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Stromal Cells ◽  
In Vitro Model ◽  
Neuroprotective Effect ◽  
Adipose Derived Stromal Cells ◽  
Vitro Model ◽  
Lateral Sclerosis

Growth factor and cytokine expression of human mesenchymal stromal cells is not altered in an in vitro model of tissue damage

Cytotherapy ◽  
2010 ◽  
Vol 12 (7) ◽  
pp. 870-880 ◽  
Author(s):  
Katrin Montzka ◽  
Tobias Führmann ◽  
Jochen Müller-Ehmsen ◽  
Michael Wöltje ◽  
Gary A. Brook
Keyword(s):  
Growth Factor ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Tissue Damage ◽  
In Vitro Model ◽  
Cytokine Expression ◽  
Human Mesenchymal Stromal Cells ◽  
Vitro Model

Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC)

2016 ◽  
Vol 133 ◽  
pp. 100-112 ◽  
Author(s):  
Victor Llombart ◽  
Teresa García-Berrocoso ◽  
Joan Josep Bech-Serra ◽  
Alba Simats ◽  
Alejandro Bustamante ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endothelial Cells ◽  
Stable Isotope ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
In Vitro Model ◽  
Isotope Labeling ◽  
Vitro Model
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