scholarly journals Reduced host cell reactivation of oxidatively damaged DNA in ageing human fibroblasts

2013 ◽  
Vol 29 (6) ◽  
pp. 2493-2497
Author(s):  
ANDREW J. RAINBOW ◽  
NATALIE J. ZACAL ◽  
DERRIK M. LEACH
Keyword(s):  
Host Cell ◽  
Human Fibroblasts ◽  
Cell Reactivation ◽  
Host Cell Reactivation ◽  
Damaged Dna

scholarly journals Exploring new potential role of DDB2 by host cell reactivation assay in human tumorigenic cells

BMC Cancer ◽  
2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Elisabetta Bassi ◽  
Paola Perucca ◽  
Isabella Guardamagna ◽  
Ennio Prosperi ◽  
Lucia A. Stivala ◽  
...  
Keyword(s):  
Dna Repair ◽  
Host Cell ◽  
Human Cells ◽  
Mutant Form ◽  
Cell Reactivation ◽  
Cellular Environment ◽  
Dna Plasmid ◽  
Host Cell Reactivation ◽  
Damaged Dna

Abstract Background The Host Cell Reactivation assay (HCR) allows studying the DNA repair capability in different types of human cells. This assay was carried out to assess the ability in removing UV-lesions from DNA, thus verifying NER efficiency. Previously we have shown that DDB2, a protein involved in the Global Genome Repair, interacts directly with PCNA and, in human cells, the loss of this interaction affects DNA repair machinery. In addition, a mutant form unable to interact with PCNA (DDB2PCNA-), has shown a reduced ability to interact with a UV-damaged DNA plasmid in vitro. Methods In this work, we have investigated whether DDB2 protein may influence the repair of a UV-damaged DNA plasmid into the cellular environment by applying the HCR method. To this end, human kidney 293 stable clones, expressing DDB2Wt or DDB2PCNA-, were co-transfected with pmRFP-N2 and UV-irradiated pEGFP-reported plasmids. Moreover, the co-localization between DDB2 proteins and different NER factors recruited at DNA damaged sites was analysed by immunofluorescence and confocal microscopy. Results The results have shown that DDB2Wt recognize and repair the UV-induced lesions in plasmidic DNA transfected in the cells, whereas a delay in these processes were observed in the presence of DDB2PCNA-, as also confirmed by the different extent of co-localization of DDB2Wt and some NER proteins (such as XPG), vs the DDB2 mutant form. Conclusion The HCR confirms itself as a very helpful approach to assess in the cellular context the effect of expressing mutant vs Wt NER proteins on the DNA damage response. Loss of interaction of DDB2 and PCNA affects negatively DNA repair efficiency.

HYPERTHERMIA AND HOST-CELL REACTIVATION OF ADENOVIRUS 12

1978 ◽  
Vol 20 (1) ◽  
pp. 35-40 ◽  
Author(s):  
P. Lam ◽  
H. F. Stich
Keyword(s):  
Host Cell ◽  
Human Fibroblasts ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Cell Populations ◽  
Hyperthermia Treatment ◽  
Cell Reactivation ◽  
Cultured Human Fibroblasts ◽  
Infected Cells ◽  
Host Cell Reactivation

Exposure of cultured human fibroblasts to hyperthermia delayed the host-cell reactivation of UV-irradiated human adenovirus type 12 (AD12). The experimental design consisted of irradiating human AD12 with UV doses ranging from 180 to 1800 ergs/mm2, infecting human cell populations at 37 °C, exposing the infected cells for 7 h to 39.5 °C and 41.8 °C, returning them to 37 °C and estimating the frequency of cells with intranuclear viral inclusion bodies (IB) 41 and 89 h after hyperthermia treatment. Hyperthermia reduced the fractions of fibroblasts with viral IB in the 41 h samples. By 89 h the capacity to form IB in the treated cells was comparable to that in control cells. Hyperthermia of 39.5 and 41.8 °C for 7 h did not affect the replication of nonirradiated AD12. The pattern of host-cell reactivation of AD12 following hyperthermia was compared to that in DNA repair deficient xeroderma pigmentosum cell populations.

scholarly journals Host cell reactivation of Bacillus subtilis bacteriophages.

1977 ◽  
Vol 131 (2) ◽  
pp. 382-388 ◽  
Author(s):  
E Ferrari ◽  
A G Siccardi ◽  
A Galizzi ◽  
U Canosi ◽  
G Mazza
Keyword(s):  
Bacillus Subtilis ◽  
Host Cell ◽  
Cell Reactivation ◽  
Host Cell Reactivation

scholarly journals Survival, mutagenesis, and host cell reactivation in a Chinese hamster ovary cell ERCC1 knock-out mutant

Mutagenesis ◽  
1997 ◽  
Vol 12 (4) ◽  
pp. 277-283 ◽  
Author(s):  
Rhonda L. Rolig ◽  
Susan K. Layher ◽  
Barbara Santi ◽  
Gerald M. Adair ◽  
Feng Gu ◽  
...  
Keyword(s):  
Host Cell ◽  
Chinese Hamster Ovary Cell ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Knock Out ◽  
Cell Reactivation ◽  
Host Cell Reactivation
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