scholarly journals MAPK inhibitors and siRNAs differentially affect cell death and ROS levels in arsenic trioxide-treated human pulmonary fibroblast cells

2012 ◽  
Author(s):  
Woo Park
Keyword(s):  
Cell Death ◽  
Arsenic Trioxide ◽  
Fibroblast Cells ◽  
Mapk Inhibitors ◽  
Pulmonary Fibroblast

Mitogen-activated protein kinase inhibitors differently affect the growth inhibition and death of a proteasome inhibitor, MG132-treated human pulmonary fibroblast cells

2011 ◽  
Vol 30 (12) ◽  
pp. 1945-1954 ◽  
Author(s):  
Woo Hyun Park
Keyword(s):  
Cell Death ◽  
Cell Growth ◽  
Growth Inhibition ◽  
Proteasome Inhibitor ◽  
Mitogen Activated Protein Kinase ◽  
Cell Growth Inhibition ◽  
P38 Inhibitor ◽  
Mitogen Activated Protein ◽  
Mapk Inhibitors ◽  
Pulmonary Fibroblast

Carbobenzoxy-Leu-Leu-leucinal (MG132) as a proteasome inhibitor can induce growth inhibition and death in lung cancer or normal cells. However, little is known about relationship between proteasome inhibition and mitogen-activated protein kinase (MAPK) signaling in normal lung cells. Thus, in the present study, we investigated the effects of MAPK inhibitors on MG132-treated human pulmonary fibroblast (HPF) cells in relation to cell growth inhibition, cell death, reactive oxygen species (ROS) and glutathione (GSH). Treatment with 15 μM MG132 increased ROS levels including mitochondrial O2•− and GSH depleted cell numbers in HPF cells at 24 hours. MAP kinase or ERK kinase (MEK) inhibitor did not significantly affect cell growth inhibition, cell death, the loss of mitochondrial membrane potential (MMP; Δ Ψm), ROS level and GSH depletion in MG132-treated HPF cells. c-Jun N-terminal kinase (JNK) inhibitor attenuated the growth inhibition and death by MG132. This inhibitor also significantly decreased O2•− level in MG132-treated HPF cells. Although p38 inhibitor slightly enhanced HPF cell growth inhibition by MG132, this inhibitor and siRNA prevented HPF cell death induced by MG132. p38 inhibitor also attenuated d O2•− level and GSH depletion. Moreover, extracellular signal regulated kinase (ERK), JNK or p38 siRNA did not strongly affect ROS levels in MG132-treated HPF cells. ERK and JNK siRNAs decreased anonymous ubiquitinated protein levels in MG132-treated HPF cells. In conclusion, MAPK inhibitors differently affected the growth inhibition and death of MG132-treated HPF cells. Especially, p38 inhibitor attenuated HPF cell death by MG132, which was in part related to changes in ROS and GSH levels.

New Multi-Walled Carbon Nanotube of industrial interest induce cell death in murine fibroblast cells

2021 ◽  
pp. 1-31
Author(s):  
Krissia Franco de Godoy ◽  
Joice Margareth de Almeida Rodolpho ◽  
Patrícia Brassolatti ◽  
Bruna Dias de Lima Fragelli ◽  
Cynthia Aparecida de Castro ◽  
...  
Keyword(s):  
Cell Death ◽  
Carbon Nanotube ◽  
Fibroblast Cells ◽  
Induce Cell Death ◽  
Multi Walled Carbon Nanotube ◽  
Murine Fibroblast

scholarly journals Inhibition of Fibroblast Apoptosis by Borrrelia afzelii, Coxiella burnetii and Bartonella henselae

2011 ◽  
Vol 60 (3) ◽  
pp. 269-272 ◽  
Author(s):  
TOMASZ CHMIELEWSKI ◽  
STANISŁAWA TYLEWSKA-WIERZBANOWSKA
Keyword(s):  
Cell Death ◽  
Chronic Disease ◽  
Coxiella Burnetii ◽  
Caspase 3 ◽  
Bartonella Henselae ◽  
Tissue Homeostasis ◽  
Host Cells ◽  
Fibroblast Cells ◽  
Human Fibroblast Cells ◽  
Long Time

Apoptosis is a genetically controlled mechanism of cell death involved in the regulation of tissue homeostasis. The aim of this study was to investigate the influence of Borrelia afzelii, Coxiella burnetii, and Bartonella henselae bacteria on apoptosis measured as the level of caspase 3 activity in human fibroblast cells HEL-299. Our findings show that C. burnetii bacteria may inhibit the process of apoptosis in the host cells for a long time. This can permit intracellular survival in the host and mediatingthe development of chronic disease.

scholarly journals Arsenic trioxide induces regulated, death receptor-independent cell death through a Bcl-2-controlled pathway

Oncogene ◽  
2005 ◽  
Vol 24 (47) ◽  
pp. 7031-7042 ◽  
Author(s):  
Christian Scholz ◽  
Antje Richter ◽  
Mario Lehmann ◽  
Klaus Schulze-Osthoff ◽  
Bernd Dörken ◽  
...  
Keyword(s):  
Cell Death ◽  
Arsenic Trioxide ◽  
Death Receptor
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