scholarly journals Impact of therapeutically induced reactive oxygen species and radical scavenging by α-tocopherol on tumor cell adhesion

2007 ◽  
Author(s):  
Oliver Thews ◽  
Christine Lambert ◽  
Debra Kelleher ◽  
Hans Biesalski ◽  
Peter Vaupel ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Adhesion ◽  
Tumor Cell ◽  
Radical Scavenging ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Tumor Cell Adhesion

scholarly journals Vesicular Formation of Trans-Ferulic Acid: an Efficient Approach to Improve the Radical Scavenging and Antimicrobial Properties

2021 ◽  
Author(s):  
Anahita Rezaeiroshan ◽  
Majid Saeedi ◽  
Katayoun Morteza-Semnani ◽  
Jafar Akbari ◽  
Akbar Hedayatizadeh-Omran ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Reactive Oxygen Species ◽  
Ferulic Acid ◽  
Reactive Oxygen Species Production ◽  
Radical Scavenging ◽  
Reactive Oxygen ◽  
Entrapment Efficiency ◽  
The Body ◽  
Oxygen Species ◽  
Species Production

Abstract Purposes Reactive oxygen species production is harmful to human’s health. The presence of antioxidants in the body may help to diminish reactive oxygen species. Trans-ferulic acid is a good antioxidant, but its low water solubility excludes its utilization. The study aims to explore whether a vesicular drug delivery could be a way to overcome the poor absorption of trans-ferulic acid hence improving its antimicrobial efficiency and antioxidant effect. Methods Niosomal vesicles containing the drug were prepared by film hydration method. The obtained vesicles were investigated in terms of morphology, size, entrapment efficiency, release behavior, cellular cytotoxicity, antioxidant, cellular protection study, and antimicrobial evaluations. Results The optimized niosomal formulation had a particle size of 158.7 nm and entrapment efficiency of 21.64%. The results showed that the optimized formulation containing 25 μM of trans-ferulic acid could enhance the viability of human foreskin fibroblast HFF cell line against reactive oxygen species production. The minimum effective dose of the plain drug and the niosomal formulation against Staphylococcus aurous (ATCC 29213) was 750 µg/mL and 375 µg/mL, respectively, and for Escherichia coli (ATCC 25922), it was 750 µg/mL and 187/5 µg/mL, respectively. The formulation could also improve the minimum bactericidal concentration of the drug in Staphylococcus aurous, Escherichia coli, and Acinobacter baumannii (ATCC 19606). Conclusion These results revealed an improvement in both antibacterial and antioxidant effects of the drug in the niosomal formulation.

Reactive oxygen species mediate Rac-induced loss of cell-cell adhesion in primary human endothelial cells

2002 ◽  
Vol 115 (9) ◽  
pp. 1837-1846 ◽  
Author(s):  
Sandra van Wetering ◽  
Jaap D. van Buul ◽  
Safira Quik ◽  
Frederik P. J. Mul ◽  
Eloise C. Anthony ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Cell Migration ◽  
Cell Adhesion ◽  
Endothelial Cell ◽  
Reactive Oxygen ◽  
Small Gtpases ◽  
Endothelial Cell Migration ◽  
Oxygen Species ◽  
Cell Cell

The integrity of the endothelium is dependent on cell-cell adhesion, which is mediated by vascular-endothelial (VE)-cadherin. Proper VE-cadherin-mediated homotypic adhesion is, in turn, dependent on the connection between VE-cadherin and the cortical actin cytoskeleton. Rho-like small GTPases are key molecular switches that control cytoskeletal dynamics and cadherin function in epithelial as well as endothelial cells. We show here that a cell-penetrating, constitutively active form of Rac (Tat-RacV12) induces a rapid loss of VE-cadherin-mediated cell-cell adhesion in endothelial cells from primary human umbilical veins (pHUVEC). This effect is accompanied by the formation of actin stress fibers and is dependent on Rho activity. However,transduction of pHUVEC with Tat-RhoV14, which induces pronounced stress fiber and focal adhesion formation, did not result in a redistribution of VE-cadherin or an overall loss of cell-cell adhesion. In line with this observation, endothelial permeability was more efficiently increased by Tat-RacV12 than by Tat-RhoV14. The loss of cell-cell adhesion, which is induced by Tat-RacV12, occurred in parallel to and was dependent upon the intracellular production of reactive oxygen species (ROS). Moreover, Tat-RacV12 induced an increase in tyrosine phosphorylation of a component the VE-cadherin-catenin complex, which was identified as α-catenin. The functional relevance of this signaling pathway was further underscored by the observation that endothelial cell migration, which requires a transient reduction of cell-cell adhesion, was blocked when signaling through ROS was inhibited. In conclusion, Rac-mediated production of ROS represents a previously unrecognized means of regulating VE-cadherin function and may play an important role in the (patho)physiology associated with inflammation and endothelial damage as well as with endothelial cell migration and angiogenesis.

Identification of the signature genes and network of Reactive Oxygen Species (ROS) related genes and DNA repair genes in Lung Adenocarcinoma (LUAD)

2021 ◽  
Author(s):  
Ye Zhao ◽  
Hai-Ming Feng ◽  
Xiao-Ping Wei ◽  
Wei-Jian Yan ◽  
Bin Li ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Dna Repair ◽  
Tumor Cell ◽  
Lung Adenocarcinoma ◽  
Tumor Cells ◽  
Patient Survival ◽  
Reactive Oxygen ◽  
Dna Repair Genes ◽  
Oxygen Species ◽  
Repair Genes

Abstract Reactive Oxygen Species (ROS) are present in high amount in patients with tumors, and these ROS can kill and destroy tumor cells. Thus, tumor cells upregulate ROS-related genes to protect themselves and reduce their destruction. Cancer cells already damaged by ROS can be repaired by expressing DNA repair genes consequently promoting their proliferation. In this work, lung adenocarcinoma (LUAD) transcriptome data in the TCGA database was analyzed and samples were clustered into 5 ROS-related categories and 6 DNA repair categories. Survival analysis revealed a significant difference in patient survival between the two classification methods. In addition, the samples corresponding to the two categories overlap, thus, the gene expression profile of the same sample with different categories and survival prognosis was further explored, and the connection between ROS-related genes and DNA repair genes was investigated. The interactive sample recombination classification was used, revealing that the patient's prognosis was worse when the ROS-related genes and DNA repair genes were expressed at the same time. The further research on the potential regulatory network of the two categories of genes and the correlation analysis revealed that ROS-related genes and DNA repair genes have a mutual regulatory relationship. The ROS-related genes NQO1, TXNRD1, and PRDX4 could establish links with other DNA repair genes through the DNA repair gene NEIL3, thereby increasing the growth of tumor cells and balancing the level of ROS, leading to tumor cell death and constant damage to the tumor cell repair system, thus prolonging patient survival. Thus, targeting ROS-related genes and DNA repair genes might be a promising strategy in the treatment of LUAD. Finally, a survival prognostic model of ROS-related genes and DNA repair genes was established (TERT, PRKDC, PTTG1, SMUG1, TXNRD1, CAT, H2AFX and PFKP), the risk score might be used as an independent prognostic factor in LUAD patients.

scholarly journals Reactive Oxygen Species as Essential Mediators of Cell Adhesion and Migration

2010 ◽  
Vol 98 (3) ◽  
pp. 576a ◽  
Author(s):  
Giuseppe Maulucci ◽  
Giovambattista Pani ◽  
Valentina Labate ◽  
Marina Mele ◽  
Emiliano Panieri ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Adhesion ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Cell Adhesion And Migration ◽  
And Migration

scholarly journals Determination of Total Phenolic, Tannin, Flavonoid Contents and Evaluation of Antioxidant Property of Amaranthus tricolor (L)

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
Sowjanya Pulipati ◽  
P. Srinivasa Babu ◽  
U. Naveena ◽  
S.K. Rafeeka Parveen ◽  
S.K. Sumaya Nausheen ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reactive Oxygen ◽  
Antioxidant Property ◽  
Total Phenolic ◽  
Oxygen Species ◽  
Amaranthus Tricolor

Free radicals or reactive oxygen species are involved in various pharmacological conditions. As synthetic antioxidants possess numerous adverse health effects, the medicinal plants possessing antioxidant components can be used to prevent harmful effects of reactive oxygen species. In the present study leaves of Amaranthus tricolor Linn were used to prepare chloroform (CEAT), methanolic (MEAT) and aqueous (AEAT) extracts, analyze the presence of phytochemicals and evaluation of in-vitro antioxidant property. Quantitative determination of phenols, tannins and flavonoids in leaves A.tricolor was carried out using spectrophotometric methods. The antioxidant activity was performed by DPPH, p-NDA radical scavenging methods for different extracts of the plant. The plant species showed that methanolic extract (MEAT) on higher concentration possess better antioxidant potential when compared with reference standard ascorbic acid. The plant extracts exhibited strong antioxidant DPPH radical scavenging activity with the IC50 values 290, 657, 830 and 130μg/ml of MEAT, CEAT, AEAT and ASA respectively. In scavenging hydroxyl radical by p-NDA method the MEAT showed maximum activity, CEAT showed moderate and AEAT showed minimum activity. The strongest antioxidant activity of MEAT could be due to the presence of flavonoids and phenols.

In- vitro Antioxidant and Cardio-protective effect of Delonix elata (L.) Leaf extract against Doxorubicin induced toxicity in H9c2 Cardio-myocyte cell line

2021 ◽  
pp. 5635-5641
Author(s):  
Archana V ◽  
Indumathy R
Keyword(s):  
Reactive Oxygen Species ◽  
Protective Effect ◽  
Cell Viability ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Reactive Oxygen ◽  
Ethanolic Extract ◽  
Antioxidant Property ◽  
Oxygen Species

Objective: The aim of this study is to evaluate the protective effect of Delonix elata (L.) leaf extract against doxorubicin-induced cardiotoxicity in H9c2 cells. Methods: Doxorubicin has been used to treat cancer, but its clinical uses are limited because of its dose-dependent cardiotoxicity. Reactive oxygen species play an important role in the pathological process of cardiotoxicity. The various extracts (pet.ether, ethyl acetate and ethanol) of Delonix elata leaves antioxidant property was evaluated by SOD antioxidant assay and DPPH free radical scavenging assay. The cells were incubated with different concentrations of various extracts of Delonix elata leaves for 2 hr, followed by incubation with 5µM doxorubicin for 24 hr. Cell viability was determined by using MTT assay, respectively. Results: The various extracts of Delonix elata leaves exhibits antioxidant activity. The Doxorubicin significantly decreased cell viability which was accompanied by an increased ROS production. Pre-treatment with various extracts of Delonix elata leaves increased the viability ofcells and inhibit the generation of reactive oxygen species. Conclusion: In this study, findings how that Delonix elata leaf extract exhibited a protective effect against oxidative stress-induced cardiomyocyte damage. The ethanolic extract of Delonix elata leaves possesses significant antioxidant and cardioprotective activity.

scholarly journals Lanthanum-Containing Magnesium Alloy with Antitumor Function Based on Increased Reactive Oxygen Species

2018 ◽  
Vol 8 (11) ◽  
pp. 2109 ◽  
Author(s):  
Cijun Shuai ◽  
Long Liu ◽  
Youwen Yang ◽  
Chengde Gao ◽  
Mingchun Zhao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Tumor Cell ◽  
Bone Tumor ◽  
Reactive Oxygen ◽  
Species Level ◽  
Reactive Oxygen Species Level ◽  
Oxygen Species ◽  
High Cell ◽  
Tumor Cell Death ◽  
Normal Cells

Developing antitumor implants is of great significance to repair tumor-induced bone defects and simultaneously prevent bone tumor recurrence. The tumor cells, compared to normal cells, have a high reactive oxygen species level. They are vulnerable to oxidative insults under increased intrinsic oxidative stress. The lanthanum (La) ion with high phospholipid binding ability can open the mitochondrial permeability transition pore, which blocks the electron transport chain in the mitochondria, and consequently increases reactive oxygen species level. In this study, La was alloyed to Mg-6Zn-0.5Zr (ZK60) through selective laser melting technology. The results indicated that the mitochondrial membrane potential dropped whilst the reactive oxygen species increased as the La content increased. ZK60-1.0La revealed a high cell inhibition rate of 61.9% for bone tumor cell and high cell viability of 91.9% for normal cells, indicating that the alloy could induce bone tumor cell death, as well as exhibit good biocompatibility for normal cell. In addition, its degradation rate 1.23 mm/year was lower than that of ZK60 alloy 2.13 mm/year, which was mainly attributed to the grain refinement.

Integrin-Mediated Cell Adhesion and Spreading Engage Different Sources of Reactive Oxygen Species

2007 ◽  
Vol 9 (4) ◽  
pp. 469-481 ◽  
Author(s):  
Maria Letizia Taddei ◽  
Matteo Parri ◽  
Tommaso Mello ◽  
Alfonso Catalano ◽  
Alan D. Levine ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Adhesion ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Different Sources
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