scholarly journals Ring finger protein 180 is associated with biological behavior and prognosis in patients with non‑small cell lung cancer

2020 ◽  
Author(s):  
Honggen Liu ◽  
Peiying Yang ◽  
Xiaojiang Li ◽  
Yingjie Jia
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Ring Finger ◽  
Small Cell ◽  
Biological Behavior ◽  
Ring Finger Protein ◽  
Small Cell Lung ◽  
Finger Protein

scholarly journals Ring finger protein 38 promote non-small cell lung cancer progression by endowing cell EMT phenotype

2018 ◽  
Vol 9 (5) ◽  
pp. 841-850 ◽  
Author(s):  
Dian Xiong ◽  
Shu-Qiang Zhu ◽  
Yong-Bing Wu ◽  
Chun Jin ◽  
Jia-Hao Jiang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cancer Progression ◽  
Cell Lung Cancer ◽  
Ring Finger ◽  
Small Cell ◽  
Ring Finger Protein ◽  
Small Cell Lung ◽  
Finger Protein

scholarly journals Expression of LUN gene that encodes a novel RING finger protein is correlated with development and progression of non-small cell lung cancer

Lung Cancer ◽  
2004 ◽  
Vol 46 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Hiroki Oyanagi ◽  
Kazumasa Takenaka ◽  
Shinya Ishikawa ◽  
Yozo Kawano ◽  
Yoshifumi Adachi ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Ring Finger ◽  
Small Cell ◽  
Ring Finger Protein ◽  
Small Cell Lung ◽  
Finger Protein

scholarly journals Ring Finger Protein 180 Suppresses Cell Proliferation and Energy Metabolism of Non-Small Cell Lung Cancer Through Down-Regulating C-myc

2020 ◽  
Author(s):  
Yi Ding ◽  
Yi Lu ◽  
Xinjie Xie ◽  
Lei Cao ◽  
Shiying Zheng
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Energy Metabolism ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Ring Finger ◽  
Small Cell ◽  
Ring Finger Protein ◽  
Small Cell Lung ◽  
Finger Protein

Abstract BackgroundNon-small cell lung cancer (NSCLC) causes a great number of cancer-related mortality worldwide, but the biomarkers for prognosis of NSCLC are scarce because of their inconsistent efficiency. Proteins containing RING finger domain are the key mediator for ubiquitination, which controls cell cycle and regulates tumor progression. Ring Finger Protein 180 (RNF180) has been reported to suppress gastric cancer, whereas its function in NSCLC is still unclear. In this study, the association between RNF180 expression and NSCLC as well as the effect of RNF180 in cellular proliferation and metabolism of NSCLC were investigated. MethodsQuantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical staining were performed to analyse RNF180 levels. Moreover, RNA interference (RNAi) and lentiviral-mediated vector transfections were performed to silence and overexpress RNF180. Further, Cell Counting Kit-8 (CCK-8) was used to assess its biological function in cell proliferation. A xenograft model was used to examine RNF180 function in vivo. ResultsWe found that the expression of RNF180 was decreased in NSCLC tissues, and its expression was positively correlated with the survival rate of NSCLC patients. Furthermore, the overexpression of RNF180 in NSCLC cells suppressed their proliferation, glycolytic activities, and mitochondrial respiration in vitro, and it restricted the tumorigenicity in mice. In addition, RNF180 knockdown promoted NSCLC cell proliferation and energy metabolism, whereas these promotive effects were counteracted by C-myc inhibitor. The underlying anti-NSCLC mechanism of RNF180 involved in the down-regulation of C-myc through ubiquitin-dependent degradation, and subsequently reduced C-myc downstream: lactate dehydrogenase-A (LDHA) and hexokinase-2 (HK2). ConclusionsThese results firstly indicated the anti-tumor properties of RNF180 and its significant correlation with NSCLC, which endorses RNF180 with the potential as efficient prognostic biomarker for tumor recurrence in NSCLC.

POM121 promotes proliferation and metastasis in non-small-cell lung cancer through TGF-β/SMAD and PI3K/AKT pathways

2021 ◽  
pp. 1-10
Author(s):  
Lirong Guan ◽  
Lingli Zhang ◽  
Tengqi Wang ◽  
Lizhou Jia ◽  
Ning Zhang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Biological Behavior ◽  
Migration And Invasion ◽  
Nuclear Pore ◽  
Tumor Diameter ◽  
Small Cell Lung ◽  
Functional Changes

BACKGROUND: Nuclear pore membrane protein 121 (POM121) is a novel biomarker involved in tumorigenesis and metastasis. However, little is known about the role of POM121 in non-small-cell lung cancer (NSCLC). OBJECTIVE: The aim of this study was to detect the expression of POM121 in NSCLC and its relationship with clinicopathologic feature and cell biological behavior, and explore the underlying mechanisms. METHODS: The expression of POM121 in NSCLC tissues and para-carcinoma tissues was compared by quantitative real-time PCR and immunohistochemistry analysis. The relationship between POM121 protein and clinicopathological characteristics in NSCLC was investigated. Roles of POM121 in NSCLC cells were investigated by CCK-8 assay, clone formation assay, transwell migration and invasion assay, and in vivo experiments. Variations of signaling pathways were determined by qRT-PCR and Western blot. RESULTS: The POM121 expression in NSCLC tissues was significantly higher than that in para-carcinoma tissues, both at the mRNA and protein level. The POM121 expression was related to sex, advanced differentiation, tumor diameter, lymph node metastases, distant metastases, American Joint Committee on Cancer (AJCC) stage, venous invasion, and perineural invasion in NSCLC. Kaplan-Meier analysis indicated that NSCLC patients with high POM121 expression had poor overall survival. Downregulation of POM121 inhibited cell proliferation, clone formation, migration and invasion. TGF-β/SMAD and PI3K/AKT pathways were involved in POM121-induced functional changes in NSCLC cells. CONCLUSION: POM121 plays an oncogenic role in NSCLC through TGF-β/SMAD and PI3K/AKT pathways. POM121 expression is a potential independent prognostic factor for NSCLC.

scholarly journals High SMARCA5 Expression is Associated With Poor Prognosis in Patients With Non-Small Cell Lung Cancer

2020 ◽  
Author(s):  
Zhi-Qiang Yang ◽  
yuanyuan wen ◽  
Hai-Feng Li ◽  
Lue Li ◽  
Hui He
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Poor Prognosis ◽  
Small Cell ◽  
High Expression ◽  
Biological Behavior ◽  
Human Lung Cancer ◽  
Small Cell Lung ◽  
Proliferation And Invasion

Abstract Background: SMARCA5 has been proved to play the role of oncogene in various types of tumors. The purpose of our study was to study the relationship between the expression of SMARCA5 and the prognosis of patients with Non-small cell lung cancer(NSCLC), and to investigate the effect of silencing the expression of SMARCA5 on the biological behavior of NSCLC. Methods：Immunohistochemistry method was adopted to detect to the expressions of SMARCA5 proteins in NSCLC and normal lung tissues. The correlation between the expression of SMARCA5 and clinicopathological factors and survival prognosis of patients with NSCLC were analyzed by statistical methods. Western blot and RT-PCR were used to detect the expression of SMARCA5 in normal human bronchial epithelial cell line (HBE) and human lung cancer cell lines (A549, Lu165, SK-MES-1 and NCI-H520). SMARCA5 siRNA was employed to silence the the expression of SMARCA5. MTT and Transwell methods were used to detect the proliferation and invasion of lung cancer cells.Results: SMARCA5 protein were highly expressed in 78 (59.5%) NSCLC tissues. SMARCA5 mRNA (P<0.05) and protein (P<0.05) were significantly higher in NSCLC tissues than in the adjacent normal tissues. SMARCA5 expression was significantly associated with poorly differentiation (P<0.001), advanced TNM stage (P<0.001), and pleural invasion (P=0.032). Meanwhile, High expression of SMARCA5 was associated with poor prognosis of NSCLC patients. Knockdown of SMARCA5 expression inhibited cell growth and invasion in NSCLC. Conclusion: High expression of SMARCA5 is associated with poor prognosis in patients with NSCLC, and SMARCA5 can increase activity of proliferation and invasion in NSCLC cells. SMARCA5 may play a decisive role in tumorigenesis of NSCLC.

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