scholarly journals Association between APOBEC3s and HPV16 E2 gene hypermutation in Uygur females with cervical cancer

2020 ◽  
Vol 20 (2) ◽  
pp. 1752-1760
Author(s):  
Shuang Sui ◽  
Zhen Jiao ◽  
Hongxiang Chen ◽  
Mayinuer Niyazi ◽  
Lin Wang
Keyword(s):  
Cervical Cancer ◽  
E2 Gene

scholarly journals Analysis by Multiplex PCR of the Physical Status of Human Papillomavirus Type 16 DNA in Cervical Cancers

1999 ◽  
Vol 37 (11) ◽  
pp. 3514-3517 ◽  
Author(s):  
Mitsuo Yoshinouchi ◽  
Atsushi Hongo ◽  
Keichiro Nakamura ◽  
Junichi Kodama ◽  
Sachio Itoh ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Multiplex Pcr ◽  
Cancer Progression ◽  
Blot Hybridization ◽  
Gene Copy ◽  
Hpv 16 ◽  
Hpv Dna ◽  
E2 Gene ◽  
Pcr Products

Integration of human papillomavirus (HPV) DNA occurs early in cancer development and is an important event in malignant transformation of cervical cancer. Integration of HPVs preferentially disrupts or deletes the E2 open reading frame, which results in the loss of its expression. The preferential disruption of the E2 gene causes the absence of the E2 gene sequences in the PCR product following integration. Twenty-two carcinomas positive for HPV type 16 (HPV-16) DNA were first tested for the disruption of the E2 gene by PCR. A specific fragment of the E2 gene was not amplified in 10 cases, suggesting integration of HPV DNA into the host genome. Next, multiplex PCR for the HPV E2 and E6 genes was carried out in the remaining 12 cases. Copy numbers of both genes should be equivalent in episomal forms, while the E2 gene copy number will be smaller than that for E6 following the preferential disruption of the E2 gene in concominant forms. Although relative ratios of HPV E2 to E6 PCR products (E2/E6 ratios) ranged from 1.40 to 2.34 in 10 of 12 cases, multiplex PCR products from 2 cases displayed extremely low ratios of 0.69 and 0.61. Southern blot hybridization with an HPV-16 probe revealed that only in these two cases was both episomal and integrated HPV DNA being carried simultaneously. Thus, multiplex PCR for the E2 and E6 genes of HPV-16 DNA following PCR for the E2 gene can distinguish the pure episomal form from a mixed form of episomal and integrated HPV DNA. Clinical application of this technique will help researchers to understand the implication of the integration of HPV DNA for cervical carcinogenesis and cervical cancer progression.

scholarly journals Enhanced oncogenicity of Asian-American human papillomavirus 16 is associated with impaired E2 repression of E6/E7 oncogene transcription

2004 ◽  
Vol 85 (6) ◽  
pp. 1433-1444 ◽  
Author(s):  
Rosa M. Ordóñez ◽  
Ana María Espinosa ◽  
Dolores Javier Sánchez-González ◽  
Juan Armendáriz-Borunda ◽  
Jaime Berumen
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Asian American ◽  
Long Control Region ◽  
Hpv 16 ◽  
E2 Protein ◽  
Human Papillomavirus 16 ◽  
E2 Gene ◽  
E7 Oncogene

Asian-American (AA) variants of human papillomavirus 16 (HPV-16) are linked to a high incidence of cervical cancer in Mexico, with some evidence strongly suggesting that they are more oncogenic than European (E) variants, including their association with younger women and their higher associated risk of cervical cancer. Differences in the regulation of viral E6/E7 oncogene transcription by the E2 protein may be involved in the higher oncogenicity of AA variants. In E variants, E6/E7 oncogene transcription is repressed by the E2 protein and is frequently up-regulated by the destruction of the E2 gene during viral integration. In contrast, the E2 gene is retained in full in most AA-positive carcinomas, raising the possibility of alternative mechanisms for increasing viral oncogene transcription. The authors investigated whether the higher oncogenicity of AA variants is linked to differences in E6/E7 oncogene transcription and the mechanism of E2 deactivation. E6/E7 and E1/E2 transcripts were explored by RT-PCR in 53 HPV-16-positive cervical carcinomas, 39 retaining (20 European and 19 AA) and 14 having lost (12 European and 2 AA) the E1/E2 genes, and transcription repression activity of the AA E2 genes was tested in four cell lines that constitutively express the β-galactosidase reporter or E6/E7 genes driven by the viral long control region. E6/E7 oncogene transcripts were found in all carcinomas, but only those positive for AA variants with E1/E2 genes had complete E2 transcripts. E2 transcripts were down-regulated by splicing in E-positive carcinomas retaining E1/E2. AA E2 genes were impaired for repression of E6/E7 oncogene transcription in vivo. These results suggest that E6/E7 oncogene expression starts earlier in AA than E variant infections, since E variants need E2 to be destroyed or down-regulated.

scholarly journals Integration of human papillomavirus type 16 in cervical cancer cells

Open Medicine ◽  
2014 ◽  
Vol 10 (1) ◽  
Author(s):  
Zivile Gudleviciene ◽  
Daiva Kanopiene ◽  
Ausra Stumbryte ◽  
Raminta Bausyte ◽  
Edgaras Kirvelaitis ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Invasive Cervical Cancer ◽  
Additional Test ◽  
Human Papillomavirus Type 16 ◽  
Non Invasive ◽  
E2 Gene ◽  
Episomal Hpv16 ◽  
The Status ◽  
Cervical Cancer Cells

AbstractCervical cancer remains an important cause of women morbidity and mortality. The progression of cervical pathology correlates with the HPV integration into the host genome. However, the data on the viral integration status in cervical dysplasias are controversial. The aim of the current study was to evaluate the status of HPV integration in two types of cervical pathology – invasive and non invasive cervical cancer (e.g. carcinoma in situ). 156 women were included in the study: 66 women were diagnosed with invasive cervical cancer (CC) and 90 with non invasive cervical cancer (carcinoma in situ, CIS). 74.2% [95% PI: 63.64÷84.76] of specimens collected from women with diagnosed CC and 85.6% [95% PI: 85.53÷92.85] of CIS specimens were positive for HPV. The most prevalent HPV genotype in both groups was HPV16. To evaluate HPV integration, three selected HPV16 E2 gene fragments were analyzed by PCR. In the majority of CC and CIS specimens the amplification of all three HPV16 E2 gene fragments was observed. The episomal HPV16 form was detected in the majority of CC and CIS specimens. The deletion of all three HPV16 E2 gene fragments was detected in 9.4% of CC specimens and 2.2% of CIS specimens. Finally, integration status could not be used as diagnostical additional test to distinguish between invasive and non invasive cervical cancer.

scholarly journals Association of viral load with HPV16 positive cervical cancer pathogenesis: Causal relevance in isolates harboring intact viral E2 gene

Virology ◽  
2010 ◽  
Vol 402 (1) ◽  
pp. 197-202 ◽  
Author(s):  
Damayanti Das ◽  
Bornali Bhattacharjee ◽  
Shrinka Sen ◽  
Indranil Mukhopadhyay ◽  
Sharmila Sengupta
Keyword(s):  
Cervical Cancer ◽  
Viral Load ◽  
Causal Relevance ◽  
E2 Gene ◽  
Cancer Pathogenesis

Human papillomavirus status in advanced cervical cancer: predictive and prognostic significance for curative radiation treatment

2005 ◽  
Vol 15 (2) ◽  
pp. 278-284 ◽  
Author(s):  
K. Lindel ◽  
P. Burri ◽  
H. U. Studer ◽  
H. J. Altermatt ◽  
R. H. Greiner ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Overall Survival ◽  
Human Papillomavirus ◽  
Progression Free Survival ◽  
Hpv Infection ◽  
Radiation Response ◽  
Advanced Cervical Cancer ◽  
Free Survival ◽  
E2 Gene ◽  
Local Progression

Human papillomavirus (HPV) infection plays a major role in oncogenesis of squamous cell carcinoma of the cervix. This study was performed to investigate if HPV status and E2 gene integrity are prognostic parameters for clinical outcome and predictive for radiation response. Forty women with locally advanced cervical cancer treated with curative radiotherapy were analyzed for HPV infection and E2 gene integrity by multiplex polymerase chain reaction. Statistical analyses were performed for overall survival, disease-free survival (DFS), local progression-free survival, and treatment response (clinical complete remission). Twenty-eight (70%) of 40 carcinomas were HPV positive. The only significant factor for a better overall survival, DFS, and local progression-free survival was HPV positivity (P < 0.02, P = 0.02, and P < 0.05, log-rank, respectively). HPV-positive tumors had a significantly better clinical complete remission (67% vs 33%, P = 0.04, Fisher's exact test). An intact E2 gene region showed a trend for a better DFS (P = 0.1, log-rank). This study reveals HPV as an independent prognostic parameter for outcome and radiation response. Integration of the virus genome into host cell DNA might be a molecular target to determine the treatment response of HPV-positive cancers.

A combined ultrastructural and gene molecular research for the relationship between human uterine cervical carcinoma and human papillomavirus

1990 ◽  
Vol 48 (3) ◽  
pp. 204-205
Author(s):  
Kun Lee ◽  
Jingyi Si ◽  
Ricai Han ◽  
Wei Zhang ◽  
Bingbing Tan ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Intraepithelial Neoplasia ◽  
Hpv Infection ◽  
Dot Blot ◽  
Homologous Sequences ◽  
Normal Cervical Epithelium ◽  
The Relationship ◽  
Chronic Cervicitis

There are more supports for the view that human papillomavirus (HPV) infection might be an etiological factor in the development of cervical cancer when the association of persistent condylomata is considered. Biopsies from 318 cases with squamous cell carcinoma of uterine cervix, 48 with cervical and vulvar condylomata, 14 with cervical intraepithelial neoplasia (CIN), 34 with chronic cervicitis and 24 normal cervical epithelium were collected from 5 geographic regions of China with different cervical cancer mortalities. All specimens were prepared for Dot blot, Southern blot and in situ DNA-DNA hybridizations by using HPV-11, 16, 18 DNA labelled with 32P and 3H as probes to detect viral homologous sequences in samples. Among them, 32 cases with cervical cancer, 27 with condyloma and 10 normal cervical epitheliums were randomly chosen for comparative EM observation. The results showed that: 1), 192 out of 318 (60.4%) cases of cervical cancer were positive for HPV-16 DNA probe (Table I)

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