scholarly journals Integrated analysis of circular RNA‑associated ceRNA network in pancreatic ductal adenocarcinoma

2020 ◽  
Author(s):  
Wei Song ◽  
Wen‑Jie Wang ◽  
Tao Fu ◽  
Lei Chen ◽  
Dong‑Liu Miao
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Circular Rna ◽  
Ductal Adenocarcinoma ◽  
Integrated Analysis ◽  
Cerna Network

scholarly journals Circular RNA circBFAR promotes the progression of pancreatic ductal adenocarcinoma via the miR-34b-5p/MET/Akt axis

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Xiaofeng Guo ◽  
Quanbo Zhou ◽  
Dan Su ◽  
Yuming Luo ◽  
Zhiqiang Fu ◽  
...  
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Circular Rna ◽  
Ductal Adenocarcinoma

Mo1344 DEVELOPMENT OF A NOVEL CIRCULAR RNA-BASED, NONINVASIVE LIQUID BIOPSY ASSAY FOR THE EARLY DETECTION OF PANCREATIC DUCTAL ADENOCARCINOMA

2020 ◽  
Vol 158 (6) ◽  
pp. S-857
Author(s):  
SOUVIK GHATAK ◽  
Satoshi Nishiwada ◽  
Eunsung Jun ◽  
Fuminori Sonohara ◽  
Yasuhiro Kodera ◽  
...  
Keyword(s):  
Early Detection ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Liquid Biopsy ◽  
Circular Rna ◽  
Ductal Adenocarcinoma

scholarly journals Integrated analysis of circular RNA-associated ceRNA network in cervical cancer

Medicine ◽  
2019 ◽  
Vol 98 (34) ◽  
pp. e16922 ◽  
Author(s):  
Jun Gong ◽  
Hui Jiang ◽  
Chang Shu ◽  
Mei-qin Hu ◽  
Yan Huang ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Circular Rna ◽  
Integrated Analysis ◽  
Cerna Network

scholarly journals CircMYOF triggers progression and facilitates glycolysis via the VEGFA/PI3K/AKT axis by absorbing miR-4739 in pancreatic ductal adenocarcinoma

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Dandan Zheng ◽  
Xianxian Huang ◽  
Juanfei Peng ◽  
Yanyan Zhuang ◽  
Yuanhua Li ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Circular Rna ◽  
Ductal Adenocarcinoma ◽  
Luciferase Reporter ◽  
Circular Rnas ◽  
Loss Of Function ◽  
Competing Endogenous Rna ◽  
Rnase R

AbstractEmerging evidence has demonstrated that circular RNAs (circRNAs) take part in the initiation and development of pancreatic ductal adenocarcinoma (PDA), a deadly neoplasm with an extremely low 5-year survival rate. Reprogrammed glucose metabolism is a key feature of tumour development, including PDA. In this research, we evaluated the role of circRNAs in reprogrammed glucose metabolism in PDA. RNA sequencing under various glucose incubation circumstances was performed. A new circMYOF was identified. Sanger sequencing and RNase R treatment confirmed its circular RNA characteristics. Real-time PCR indicated that it was highly expressed in PDA clinical specimens and cell lines. Gain-of- and loss-of-function assays showed that circMYOF induced progression in PDA. Mechanistically, RNA pull-down and luciferase reporter experiments elucidated that circMYOF, as a competing endogenous RNA for miR-4739, facilitated glycolysis via the VEGFA/PI3K/AKT pathway. Taken together, our findings indicate that circMYOF may work as a desirable biomarker and therapeutic target for PDA patients.

scholarly journals Circular RNA Expression Profile of Pancreatic Ductal Adenocarcinoma Revealed by Microarray

2016 ◽  
Vol 40 (6) ◽  
pp. 1334-1344 ◽  
Author(s):  
Haimin Li ◽  
Xiaokun Hao ◽  
Huimin Wang ◽  
Zhengcai Liu ◽  
Yong He ◽  
...  
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Expression Profile ◽  
Mammalian Cells ◽  
Noncoding Rna ◽  
Circular Rna ◽  
Pathological Process ◽  
Ductal Adenocarcinoma ◽  
Circular Rnas ◽  
Normal Tissues ◽  
Polymerase Chain

Background/Aims: Circular RNAs (circRNAs) are a special novel type of a stable, diverse and conserved noncoding RNA in mammalian cells. Particularly in cancer, circRNAs have been reported to be widely involved in the physiological/pathological process of life. However, it is unclear whether circRNAs are specifically involved in pancreatic ductal adenocarcinoma (PDAC). Methods: We investigated the expression profile of circRNAs in six PDAC cancer samples and paired adjacent normal tissues using microarray. A high-throughput circRNA microarray was used to identify dysregulated circular RNAs in six PDAC patients. Bioinformatic analyses were applied to study these differentially expressed circRNAs. Furthermore, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to confirm these results. Results: We revealed and confirmed that a number of circRNAs were dysregulated, which suggests a potential role in pancreatic cancer. Conclusions: this study demonstrates that clusters of circRNAs are aberrantly expressed in PDAC compared with normal samples and provides new potential targets for the future treatment of PDAC and novel insights into PDAC biology.

scholarly journals Integrated Analysis of Circular RNA-Associated ceRNA Network Reveals Potential circRNA Biomarkers in Human Breast Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Han Sheng ◽  
Huan Pan ◽  
Ming Yao ◽  
Longsheng Xu ◽  
Jianju Lu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Survival Analysis ◽  
Correlation Analysis ◽  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Circular Rna ◽  
Functional Enrichment ◽  
Differentially Expressed ◽  
Integrated Analysis ◽  
Cerna Network

Circular RNA (circRNA) is closely related to tumorigenesis and cancer progression. Yet, the roles of cancer-specific circRNAs in the circRNA-related ceRNA network of breast cancer (BRCA) remain unclear. The aim of this study was to construct a ceRNA network associated with circRNA and to explore new therapeutic and prognostic targets and biomarkers for breast cancer. We downloaded the circRNA expression profile of BRCA from Gene Expression Omnibus (GEO) microarray datasets and downloaded the miRNA and mRNA expression profiles of BRCA from The Cancer Genome Atlas (TCGA) database. Differentially expressed mRNAs (DEmRNAs), differentially expressed miRNAs (DEmiRNAs), and differentially expressed circRNAs (DEcircRNAs) were identified, and a competitive endogenous RNA (ceRNA) regulatory network was constructed based on circRNA–miRNA pairs and miRNA–mRNA pairs. Gene ontology and pathway enrichment analyses were performed on mRNAs regulated by circRNAs in ceRNA networks. Survival analysis and correlation analysis of all mRNAs and miRNAs in the ceRNA network were performed. A total of 72 DEcircRNAs, 158 DEmiRNAs, and 2762 DE mRNAs were identified. The constructed ceRNA network contains 60 circRNA–miRNA pairs and 140 miRNA–mRNA pairs, including 40 circRNAs, 30 miRNAs, and 100 mRNAs. Functional enrichment indicated that DEmRNAs regulated by DEcircRNAs in ceRNA networks were significantly enriched in the PI3K-Akt signaling pathway, microRNAs in cancer, and proteoglycans in cancer. Survival analysis and correlation analysis of all mRNAs and miRNAs in the ceRNA network showed that 13 mRNAs and 6 miRNAs were significantly associated with overall survival, and 48 miRNA–mRNA interaction pairs had a significant negative correlation. A PPI network was established, and 21 hub genes were determined from the network. This study provides an effective bioinformatics basis for further understanding of the molecular mechanisms and predictions of breast cancer. A better understanding of the circRNA-related ceRNA network in BRCA will help identify potential biomarkers for diagnosis and prognosis.

scholarly journals The Role of Circular RNAs in Pancreatic Ductal Adenocarcinoma and Biliary-Tract Cancers

Cancers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3250
Author(s):  
Christopher Limb ◽  
Daniel S. K. Liu ◽  
Morten T. Veno ◽  
Eleanor Rees ◽  
Jonathan Krell ◽  
...  
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Biliary Tract ◽  
Ex Vivo ◽  
Expression Profiles ◽  
Ductal Adenocarcinoma ◽  
Circular Rnas ◽  
Specific Expression ◽  
Biliary Tract Cancers ◽  
Cerna Network

Pancreatic Ductal Adenocarcinoma (PDAC) and biliary-tract cancers (BTC) often present at a late stage, and consequently patients have poor survival-outcomes. Circular RNAs (circRNAs) are non-coding RNA molecules whose role in tumourigenesis has recently been realised. They are stable, conserved and abundant, with tissue-specific expression profiles. Therefore, significant interest has arisen in their use as potential biomarkers for PDAC and BTC. High-throughput methods and more advanced bioinformatic techniques have enabled better profiling and progressed our understanding of how circRNAs may function in the competing endogenous RNA (ceRNA) network to influence the transcriptome in these cancers. Therefore, the aim of this systematic review was to describe the roles of circRNAs in PDAC and BTC, their potential as biomarkers, and their function in the wider ceRNA network in regulating microRNAs and the transcriptome. Medline, Embase, Scopus and PubMed were systematically reviewed to identify all the studies addressing circRNAs in PDAC and BTC. A total of 32 articles were included: 22 considering PDAC, 7 for Cholangiocarcinoma (CCA) and 3 for Gallbladder Cancer (GBC). There were no studies investigating Ampullary Cancer. Dysregulated circRNA expression was associated with features of malignancy in vitro, in vivo, and ex vivo. Overall, there have been very few PDAC and BTC tissues profiled for circRNA signatures. Therefore, whilst the current studies have demonstrated some of their functions in these cancers, further work is required to elucidate their potential role as cancer biomarkers in tissue, biofluids and biopsies.

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