scholarly journals Gene expression analyses associated with malignant phenotypes of metastatic sub-clones derived from a mouse oral squamous cell carcinoma Sq-1979 cell line

2017 ◽  
Author(s):  
Mitsutaka Adachi ◽  
Masako Mizuno‑Kamiya ◽  
Eiji Takayama ◽  
Harumi Kawaki ◽  
Toshihiro Inagaki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Squamous Cell ◽  
Gene Expression Analyses

scholarly journals GANT61 Reduces Hedgehog Molecule (GLI1) Expression and Promotes Apoptosis in Metastatic Oral Squamous Cell Carcinoma Cells

2020 ◽  
Vol 21 (17) ◽  
pp. 6076
Author(s):  
Taís Bacelar Sacramento de Araújo ◽  
Leonardo de Oliveira Siquara da Rocha ◽  
Manuela Torres Andion Vidal ◽  
Paulo Lucas Cerqueira Coelho ◽  
Mitermayer Galvão dos Reis ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Cell Death ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Viability ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Squamous Cell ◽  
Nuclear Fragmentation

Due to its importance in the pathogenesis of oral squamous cell carcinoma (OSCC), the Hedgehog (HH) pathway is considered a potential therapeutic target. We investigated the effects of GANT61, a GLI inhibitor, on HH gene expression, as well as on metastatic OSCC cell proliferation and death. Following culture in DMEM medium, cytotoxicity of GANT61 against different tumor and non-tumor cell types was assessed by alamarBlue assays. Cytotoxicity analysis revealed that the metastatic HSC3 cell line was the most sensitive (IC50: 36 µM) to the tested compound. The compound’s effects on the expression of HH pathways components were analyzed by qPCR and Western blot; cell viability was analyzed by trypan blue assay and flow cytometry were used to investigate cell cycle phase, morphology, and death patterns in HSC3 cells. A significant reduction in mRNA levels of the GLI1 transcription factor was found after 12 h of treatment withGANT61. Protein expression levels of other HH pathway components (PTCH1, SHH, and Gli1) and HSC3 cell viability also decreased after 24 h of treatment. Cell cycle analysis and death pattern evaluations revealed significantly increased nuclear fragmentation in sub-G1 phase, as well as cell death due to apoptosis. In conclusion, the significantly reduced GLI1 gene expression seen in response to the GLI inhibitor indicates diminished downstream activation in HH pathway components. GANT61 significantly reduced cell viability in the metastatic cell line of OSCC and promoted a significant increase in nuclear fragmentation and cell death by apoptosis.

Evaluation of the Cytotoxic Activity of Crocin and Safranal, Constituents of Saffron, in Oral Squamous Cell Carcinoma (KB Cell Line)

2017 ◽  
Vol 69 (6) ◽  
pp. 911-919 ◽  
Author(s):  
Raheleh Jabini ◽  
Melika Ehtesham-Gharaee ◽  
Zohreh Dalirsani ◽  
Fatemeh Mosaffa ◽  
Zahra Delavarian ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cytotoxic Activity ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Squamous Cell

scholarly journals Gene Expression of Cancer Stem Cell in Oral Squamous Cell Carcinoma

2012 ◽  
Vol 32 (2) ◽  
pp. 81-89
Author(s):  
Tomohide ISOBE ◽  
Gou YAMAMOTO ◽  
Tarou IRIE ◽  
Tetuhiko TACHIKAWA ◽  
Kenji MISHIMA
Keyword(s):  
Gene Expression ◽  
Squamous Cell Carcinoma ◽  
Stem Cell ◽  
Oral Squamous Cell Carcinoma ◽  
Cancer Stem Cell ◽  
Cell Carcinoma ◽  
Squamous Cell

scholarly journals AIRE is induced in oral squamous cell carcinoma and promotes cancer gene expression

PLoS ONE ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. e0222689
Author(s):  
Chi Thi Kim Nguyen ◽  
Wanlada Sawangarun ◽  
Masita Mandasari ◽  
Kei-ichi Morita ◽  
Hiroyuki Harada ◽  
...  
Keyword(s):  
Gene Expression ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cancer Gene
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