scholarly journals Patuletin induces apoptosis of human breast cancer SK‑BR‑3 cell line via inhibiting fatty acid synthase gene expression and activity

2017 ◽  
Author(s):  
Wanwan Zhu ◽  
Chunmei Lv ◽  
Jiao Wang ◽  
Qiang Gao ◽  
Hui Zhu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Fatty Acid ◽  
Cell Line ◽  
Human Breast Cancer ◽  
Fatty Acid Synthase ◽  
Human Breast ◽  
Expression And Activity ◽  
Fatty Acid Synthase Gene

scholarly journals Histone Deacetylase Inhibitors and Microtubule Inhibitors Induce Apoptosis in Feline Luminal Mammary Carcinoma Cells

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 502
Author(s):  
Filipe Almeida ◽  
Andreia Gameiro ◽  
Jorge Correia ◽  
Fernando Ferreira
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cell Line ◽  
Cell Lines ◽  
Mammary Carcinoma ◽  
Human Breast Cancer ◽  
Histone Deacetylase Inhibitors ◽  
Trichostatin A ◽  
Human Breast ◽  
Antitumor Effects

Feline mammary carcinoma (FMC) is the third most common type of neoplasia in cats, sharing similar epidemiological features with human breast cancer. In humans, histone deacetylases (HDACs) play an important role in the regulation of gene expression, with HDAC inhibitors (HDACis) disrupting gene expression and leading to cell death. In parallel, microtubules inhibitors (MTIs) interfere with the polymerization of microtubules, leading to cell cycle arrest and apoptosis. Although HDACis and MTIs are used in human cancer patients, in cats, data is scarce. In this study, we evaluated the antitumor properties of six HDACis (CI-994, panobinostat, SAHA, SBHA, scriptaid, and trichostatin A) and four MTIs (colchicine, nocodazole, paclitaxel, and vinblastine) using three FMC cell lines (CAT-MT, FMCp, and FMCm), and compared with the human breast cancer cell line (SK-BR-3). HDACis and MTIs exhibited dose-dependent antitumor effects in FMC cell lines, and for all inhibitors, the IC50 values were determined, with one feline cell line showing reduced susceptibility (FMCm). Immunoblot analysis confirmed an increase in the acetylation status of core histone protein HDAC3 and flow cytometry showed that HDACis and MTIs lead to cellular apoptosis. Overall, our study uncovers HDACis and MTIs as promising anti-cancer agents to treat FMCs.

Fatty acid synthase (FAS) expression in human breast cancer cell culture supernatants and in breast cancer patients

2001 ◽  
Vol 167 (1) ◽  
pp. 99-104 ◽  
Author(s):  
Young (Yongqiang) Wang ◽  
Francis P. Kuhajda ◽  
Ji Nong Li ◽  
Ellen S. Pizer ◽  
Wan Fang Han ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Fatty Acid ◽  
Human Breast Cancer ◽  
Fatty Acid Synthase ◽  
Human Breast Cancer Cell ◽  
Breast Cancer Patients ◽  
Human Breast ◽  
Breast Cancer Cell Culture ◽  
Culture Supernatants

scholarly journals Leucine deprivation inhibits proliferation and induces apoptosis of human breast cancer cells via fatty acid synthase

Oncotarget ◽  
2016 ◽  
Vol 7 (39) ◽  
pp. 63679-63689 ◽  
Author(s):  
Fei Xiao ◽  
Chunxia Wang ◽  
Hongkun Yin ◽  
Junjie Yu ◽  
Shanghai Chen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Fatty Acid ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Fatty Acid Synthase ◽  
Human Breast Cancer Cells ◽  
Human Breast

scholarly journals Screening to Identify Commonly Used Chinese Herbs That AffectERBB2andESR1Gene Expression Using the Human Breast Cancer MCF-7 Cell Line

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Jen-Hwey Chiu ◽  
Chun-Ju Chang ◽  
Jing-Chong Wu ◽  
Hui-Ju Liu ◽  
Che-Sheng Wen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Protein Expression ◽  
Cell Line ◽  
Human Breast Cancer ◽  
Luciferase Activity ◽  
Chinese Herbs ◽  
Herbal Extracts ◽  
Human Breast ◽  
Mcf 7

Aim. Our aim the was to screen the commonly used Chinese herbs in order to detect changes inERBB2andESR1gene expression using MCF-7 cells.Methods. Using the MCF-7 human breast cancer cell line, cell cytotoxicity and proliferation were evaluated by MTT and trypan blue exclusion assays, respectively. A luciferase reporter assay was established by transient transfecting MCF-7 cells with plasmids containing either theERBB2or theESR1promoter region linked to the luciferase gene. Chinese herbal extracts were used to treat the cells at 24 h after transfection, followed by measurement of their luciferase activity. The screening results were verified by Western blotting to measure HER2 and ERαprotein expression.Results. At concentrations that induced little cytotoxicity, thirteen single herbal extracts and five compound recipes were found to increase eitherERBB2orESR1luciferase activity. By Western blotting, Si-Wu-Tang, Kuan-Shin-Yin, and Suan-Tsao-Ren-Tang were found to increase either HER2 or ERαprotein expression. In addition,Ligusticum chuanxiongwas shown to have a great effect onERBB2gene expression and synergistically with estrogen to stimulate MCF-7 cell growth.Conclusion. Our results provide important information that should affect clinical treatment strategies among breast cancer patients who are receiving hormonal or targeted therapies.

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