scholarly journals Validation of reference genes for the normalization of RT-qPCR expression studies in human tongue carcinoma cell lines and tissue

2017 ◽  
Vol 13 (5) ◽  
pp. 3951-3957 ◽  
Author(s):  
Xiaofeng Wang ◽  
Xin Liu ◽  
Cong Liu ◽  
Ming Ren ◽  
Sujie Gao ◽  
...  
Keyword(s):  
Cell Lines ◽  
Reference Genes ◽  
Carcinoma Cell ◽  
Tongue Carcinoma ◽  
Carcinoma Cell Lines ◽  
Human Tongue ◽  
Expression Studies

scholarly journals The expression of the GATA6 gene in oral carcinoma cell lines

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Cheng-Lin Xu ◽  
Wei-Qun Guan ◽  
Xue-Ying Wang
Keyword(s):  
Cell Lines ◽  
Carcinoma Cell ◽  
Carcinoma Cells ◽  
Expression Levels ◽  
Carcinoma Cell Lines ◽  
Human Tongue ◽  
Adenoid Cystic ◽  
Significant Difference ◽  
Oral Epithelial ◽  
Gata6 Gene

Abstract Background This study aimed to investigate the expression level of the GATA6 gene in different oral cancer cells. Methods In this study, we sub-cultured normal oral epithelial cell lines HOK, human tongue squamous cell carcinoma cell lines CAL-27 and SCC-4, and human salivary gland adenoid cystic carcinoma cell lines SACC-LM and SACC-83. Subsequently, we used reverse transcription-polymerase chain reaction RT-PCR and Western blot methods to detect the mRNA and the protein expressions of GATA6 in normal oral epithelial cells, human tongue squamous cell carcinoma cells, and human salivary gland adenoid cystic carcinoma cells. Results The results of this study showed that the mRNA expression levels of GATA6 in CAL-27, SCC-4, and SACC-LM cells were significantly increased when compared with the HOK cells. However, the mRNA expression level of GATA6 in the SACC-83 cells had no significant difference compared with the HOK cells. The protein expression levels of GATA6 in the SCC-4 and SACC-LM cells were, however, significantly increased whereas the protein expression levels of GATA6 in the CAL-27 and SACC-83 cells had no significant difference when compared with the HOK cells. Conclusion The GATA6 gene may be related to the occurrence and progression of certain oral cancers.

Part II. Initial molecular and cellular characterization of high nitric oxide-adapted human tongue squamous cell carcinoma cell lines

Tumor Biology ◽  
2010 ◽  
Vol 32 (1) ◽  
pp. 87-98 ◽  
Author(s):  
Gabor Tarjan ◽  
G. Kenneth Haines ◽  
Benjamin J. Vesper ◽  
Jiaping Xue ◽  
Michael B. Altman ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Lines ◽  
Squamous Cell ◽  
Carcinoma Cell ◽  
Squamous Cell Carcinoma Cell ◽  
Carcinoma Cell Lines ◽  
Human Tongue

scholarly journals Model of human tongue squamous cell line stably transfected with human papillomavirus（HPV) 16 E6 and E7 genes and biological characteristic analysis

2020 ◽  
Author(s):  
Zilian Lan ◽  
Ziyao Jia ◽  
Hengyuan Guo ◽  
Zhaoshou Yang ◽  
Zifan Yang ◽  
...  
Keyword(s):  
Cell Lines ◽  
Carcinoma Cell ◽  
Squamous Carcinoma ◽  
Migration Ability ◽  
Carcinoma Cell Lines ◽  
Human Tongue ◽  
Squamous Carcinoma Cell ◽  
And Migration ◽  
Tongue Squamous Carcinoma ◽  
Proliferation And Migration

Abstract Human tongue squamous carcinoma cell lines transfected with HPV16E6E7 gene were established to provide a model for further study of HPV16 E6E7-related human tongue squamous carcinoma cell lines .Plasmid pEGFR/HPV16 of E6E7 and plasmid pEGFR/HPV16 of No E6E7 were constructed.Human tongue squamous carcinoma cell lines including SCC9 and SCC15 were infected by liposome transfection and would be highly selected by antibiotic .Fluorescence imaging, RT-PCR and Westernblot were used to detect the expression of HPV16 E6E7 in cells.The biological characteristics of human tongue squamous carcinoma cell lines infected with HPV16 E6E7 were detected by CCK-8 and wound healing assay. The human tongue squamous carcinoma cell lines transfected with HPV16 E6E7 gene were successfully established and identified, and the proliferation and migration ability of the human tongue squamous carcinoma cell lines infected with HPV16 E6E7 gene was significantly stronger than that of the blank group.Human tongue squamous carcinoma cell lines infected with HPV16 E6E7 were more malignant, and their proliferation and migration ability were higher than those not infected with HPV16 E6E7.

Expression of the cell–cell adhesion molecule E-cadherin in tongue carcinoma cell lines

1994 ◽  
Vol 108 (11) ◽  
pp. 957-961 ◽  
Author(s):  
A. R. Kinsella ◽  
G. L. Bowie ◽  
J. K. Fields ◽  
A. S. Jones
Keyword(s):  
Cell Adhesion ◽  
Cell Line ◽  
Cell Lines ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Carcinoma Cell ◽  
Tumour Progression ◽  
Tongue Carcinoma ◽  
Carcinoma Cell Lines ◽  
E Cadherin

AbstractA reduction in cell adhesiveness and cell invasion are essential steps in tumour progression. In the present study six tongue carcinoma cell lines were compared with regard to their invasive potential in two in vitro invasion assay systems and for their patterns of expression of the cell–cell adhesion molecule E-cadherin. The three cell lines negative for E-cadherin expression were invasive in both assays. One cell line with strong E-cadherin expression was strongly invasive and one weakly invasive. One cell line with reduced E-cadherin expression was weakly invasive. There was no significant pattern to these findings (x2 = 0.375; p = 0.54). This supports previous studies from this group that suggest that E-cadherin is only one of the presumably many molecules involved in tumour progression in squamous cell carcinoma of the tongue.

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