scholarly journals Anticancer effect of icaritin inhibits cell growth of colon cancer through reactive oxygen species, Bcl-2 and cyclin D1/E signaling

2016 ◽  
Vol 12 (5) ◽  
pp. 3537-3542 ◽  
Author(s):  
Chaofeng Li ◽  
Weichao Peng ◽  
Xin Song ◽  
Qian Wang ◽  
Wenyue Wang
Keyword(s):  
Reactive Oxygen Species ◽  
Colon Cancer ◽  
Cell Growth ◽  
Cyclin D1 ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Anticancer Effect

scholarly journals Competitive Nuclear Export of Cyclin D1 and Hic-5 Regulates Anchorage Dependence of Cell Growth and Survival

2009 ◽  
Vol 20 (1) ◽  
pp. 218-232 ◽  
Author(s):  
Kazunori Mori ◽  
Etsuko Hirao ◽  
Yosuke Toya ◽  
Yukiko Oshima ◽  
Fumihiro Ishikawa ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Growth ◽  
Cyclin D1 ◽  
Nuclear Export ◽  
Reactive Oxygen ◽  
Adaptor Protein ◽  
Oxygen Species ◽  
Growth And Survival ◽  
Nonadherent Cells ◽  
Cell Growth And Survival

Anchorage dependence of cell growth and survival is a critical trait that distinguishes nontransformed cells from transformed cells. We demonstrate that anchorage dependence is determined by anchorage-dependent nuclear retention of cyclin D1, which is regulated by the focal adhesion protein, Hic-5, whose CRM1-dependent nuclear export counteracts that of cyclin D1. An adaptor protein, PINCH, interacts with cyclin D1 and Hic-5 and potentially serves as an interface for the competition between cyclin D1 and Hic-5 for CRM1. In nonadherent cells, the nuclear export of Hic-5, which is redox-sensitive, was interrupted due to elevated production of reactive oxygen species, and cyclin D1 was exported from the nucleus. When an Hic-5 mutant that was continuously exported in a reactive oxygen species-insensitive manner was introduced into the cells, cyclin D1 was retained in the nucleus under nonadherent conditions, and a significant population of cells escaped from growth arrest or apoptosis. Interestingly, activated ras achieved predominant cyclin D1 nuclear localization and thus, growth in nonadherent cells. We report a failsafe system for anchorage dependence of cell growth and survival.

Reactive oxygen species produced by NADPH oxidase regulate plant cell growth

Nature ◽  
2003 ◽  
Vol 422 (6930) ◽  
pp. 442-446 ◽  
Author(s):  
Julia Foreman ◽  
Vadim Demidchik ◽  
John H. F. Bothwell ◽  
Panagiota Mylona ◽  
Henk Miedema ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Growth ◽  
Nadph Oxidase ◽  
Plant Cell ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Plant Cell Growth

scholarly journals Ethanol extract of Chrysanthemum zawadskii Herbich induces autophagy and apoptosis in mouse colon cancer cells through the regulation of reactive oxygen species

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Kwang-Youn Kim ◽  
Tae-Woo Oh ◽  
Hye-Jin Yang ◽  
Young-Woo Kim ◽  
Jin-Yeul Ma ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Colon Cancer ◽  
Cancer Cells ◽  
Reactive Oxygen ◽  
Ethanol Extract ◽  
Oxygen Species ◽  
Colon Cancer Cells ◽  
Mouse Colon ◽  
Induced Apoptosis ◽  
Chrysanthemum Zawadskii

Abstract Background Recent research has suggested that autophagy can provide a better mechanism for inducing cell death than current therapeutic strategies. This study investigated the effects of using an ethanol extract of Chrysanthemum zawadskii Herbich (ECZ) to induce apoptosis and autophagy associated with reliable signal pathways in mouse colon cancer CT-26 cells. Methods Using ECZ on mouse colon cancer CT-26 cells, cell viability, annexin V/propidium iodide staining, acridine orange staining, reactive oxygen species (ROS) and western blotting were assayed. Results ECZ exhibited cytotoxicity in CT-26 cells in a dose-dependent manner. ECZ induced apoptosis was confirmed by caspase-3 activation, poly (ADP-ribose) polymerase cleavage, and increased production of reactive oxygen species (ROS). Furthermore, it was shown that ECZ induced autophagy via the increased conversion of microtubule-associated protein 1 light chain 3II, the degradation of p62, and the formation of acidic vesicular organelles. The inhibition of ROS production by N-Acetyl-L-cysteine resulted in reduced ECZ-induced apoptosis and autophagy. Furthermore, the inhibition of autophagy by 3-methyladenine resulted in enhanced ECZ-induced apoptosis via increased ROS generation. Conclusion These findings confirmed that ECZ induced ROS-mediated autophagy and apoptosis in colon cancer cells. Therefore, ECZ may serve as a novel potential chemotherapeutic candidate for colon cancer.

scholarly journals Novel Anthraquinone Compounds Inhibit Colon Cancer Cell Proliferation via the Reactive Oxygen Species/JNK Pathway

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1672 ◽  
Author(s):  
Yuying Li ◽  
Fang Guo ◽  
Yingying Guan ◽  
Tinggui Chen ◽  
Kaiqing Ma ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Colon Cancer ◽  
Cancer Cell ◽  
Human Cancer ◽  
Hct116 Cell ◽  
Cell Activity ◽  
Similarity Index ◽  
Reactive Oxygen ◽  
Field Analysis ◽  
Oxygen Species

A series of amide anthraquinone derivatives, an important component of some traditional Chinese medicines, were structurally modified and the resulting antitumor activities were evaluated. The compounds showed potent anti-proliferative activities against eight human cancer cell lines, with no noticeable cytotoxicity towards normal cells. Among the candidate compounds, 1-nitro-2-acyl anthraquinone-leucine (8a) showed the greatest inhibition of HCT116 cell activity with an IC50 of 17.80 μg/mL. In addition, a correlation model was established in a three-dimensional quantitative structure-activity relationship (3D-QSAR) study using Comparative Molecular Field Analysis (CoMFA) and comparative molecular similarity index analysis (CoMSIA). Moreover, compound 8a effectively killed tumor cells by reactive oxygen species (ROS)-JNK activation, causing an increase in ROS levels, JNK phosphorylation, and mitochondrial stress. Cytochrome c was then released into cytoplasm, which, in turn activated the cysteine protease pathway and ultimately induced tumor cell apoptosis, suggesting a potential use of this compound for colon cancer treatment.

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