scholarly journals Integrin β1-mediated acquired gefitinib resistance in non-small cell lung cancer cells occurs via the phosphoinositide 3-kinase-dependent pathway

2015 ◽  
Vol 11 (1) ◽  
pp. 535-542 ◽  
Author(s):  
QIN-FANG DENG ◽  
BO SU ◽  
YIN-MIN ZHAO ◽  
LIANG TANG ◽  
JIE ZHANG ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cancer Cells ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Small Cell Lung ◽  
Integrin Β1 ◽  
Phosphoinositide 3 Kinase ◽  
Gefitinib Resistance ◽  
Dependent Pathway

scholarly journals ERK inhibition represses gefitinib resistance in non-small cell lung cancer cells

Oncotarget ◽  
2018 ◽  
Vol 9 (15) ◽  
pp. 12020-12034 ◽  
Author(s):  
Mengfan Qi ◽  
Ye Tian ◽  
Wang Li ◽  
Dan Li ◽  
Tian Zhao ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cancer Cells ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Lung Cancer Cells ◽  
Small Cell Lung ◽  
Erk Inhibition ◽  
Gefitinib Resistance

β-Catenin overexpression is associated with gefitinib resistance in non-small cell lung cancer cells

2014 ◽  
Vol 28 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Xia Fang ◽  
Pan Gu ◽  
Caicun Zhou ◽  
Aibin Liang ◽  
Shenxiang Ren ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cancer Cells ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Lung Cancer Cells ◽  
Small Cell Lung ◽  
Gefitinib Resistance

scholarly journals Genome-wide profiling of micro-RNA expression in gefitinib-resistant human lung adenocarcinoma using microarray for the identification of miR-149-5p modulation

Tumor Biology ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 101042831769165 ◽  
Author(s):  
Yong Hu ◽  
Xiaobing Qin ◽  
Dali Yan ◽  
Haixia Cao ◽  
Leilei Zhou ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Micro Rna ◽  
Lung Cancer Cells ◽  
Small Cell Lung ◽  
Gefitinib Resistance

To understand the mechanism involved in gefitinib resistance, we established gefitinib-resistant human HCC827/GR-8-1 cell line from the parental HCC827 cell line. We compared the micro-RNA expression profiles of the HCC827 cells HCC827/GR-8-1 using Agilent micro-RNA microarrays. The micro-RNAs, such as the miR-149-5p, were up- or downregulated and associated with acquired gefitinib resistance. Quantitative real-time polymerase chain reaction was then performed to verify the expression patterns of different micro-RNAs. The result showed that miR-149-5p was upregulated in the HCC827/GR-8-1 cell line. To investigate the biological function of miR-149-5p in non–small cell lung cancer cells acquired gefitinib resistance, we examined cell proliferation using a cell counting kit-8 assay. Cell viability was evaluated after the miR-149-5p mimics, inhibitors, and negative control were separately transfected into the non–small cell lung cancer cells. The results showed that the non–small cell lung cancer cells transfected with miR-149-5p mimics exhibited reduced cell motility. The drug-sensitivity assay results revealed that the overexpression of miR-149-5p effectively evaluates the half maximal inhibitory concentration values of the cell in response to gefitinib, and the downregulation of miR-149-5p can attenuate the half maximal inhibitory concentration values of the cell lines in response to gefitinib. Furthermore, the levels of miR-149-5p in the HCC827 and HCC827/GR-8-1 cells were inversely correlated with caspase-3 expression. In conclusion, this study revealed that miR-149-5p is upregulated in the HCC827/GR-8-1 cells and involved in the acquired gefitinib resistance.

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