scholarly journals Effect of adipose-derived stem cell-conditioned medium on the proliferation and migration of B16 melanoma cells

2015 ◽  
Vol 10 (2) ◽  
pp. 730-736 ◽  
Author(s):  
JU-HEE LEE ◽  
CHUL HONG PARK ◽  
KWANG-HOON CHUN ◽  
SOON-SUN HONG
Keyword(s):  
Stem Cell ◽  
Conditioned Medium ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
B16 Melanoma Cells ◽  
And Migration ◽  
Adipose Derived Stem Cell ◽  
Cell Conditioned Medium ◽  
Proliferation And Migration

scholarly journals The Effects of Cytokines in Adipose Stem Cell-Conditioned Medium on the Migration and Proliferation of Skin FibroblastsIn Vitro

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Jiajia Zhao ◽  
Li Hu ◽  
Jiarong Liu ◽  
Niya Gong ◽  
Lili Chen
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Conditioned Medium ◽  
Healing Process ◽  
Dermal Fibroblasts ◽  
Wound Healing Process ◽  
Adipose Stem Cell ◽  
And Migration ◽  
Cell Conditioned Medium ◽  
Proliferation And Migration

Although adipose stem cell-conditioned medium (ASC-CM) has demonstrated the effect of promoting the cutaneous wound healing, the mechanism for this response on the effector cells (e.g., dermal fibroblasts) during the process remains to be determined. In this study, we aim to investigate the types and contents of cytokines in ASC-CM and the effects of some kinds of common cytokines in ASC-CM, such as EGF, PDGF-AA, VEGF, and bFGF, on dermal fibroblasts proliferation and migration in wound healing process. Results showed that these four cytokines had high concentrations in ASC-CM. The migration of skin fibroblasts could be significantly stimulated by VEGF, bFGF, and PDGF-AA, and the proliferation could be significantly stimulated by bFGF and EGF in ASC-CM. Additionally, ASC-CM had more obvious promoting effect on fibroblasts proliferation and migration than single cytokine. These observations suggested that ASC-CM played an important role in the cutaneous injury partly by the synergistic actions of several cytokines in promoting dermal fibroblasts proliferation and migration, and ASC-CM was more adaptive than each single cytokine to be applied in promoting the wound healing.

Sequential Scalp Assessment in Hair Regeneration Therapy Using an Adipose-Derived Stem Cell–Conditioned Medium

2020 ◽  
Vol 46 (6) ◽  
pp. 819-825 ◽  
Author(s):  
Keigo Narita ◽  
Hirotaro Fukuoka ◽  
Takuya Sekiyama ◽  
Hirotaka Suga ◽  
Kiyonori Harii
Keyword(s):  
Stem Cell ◽  
Conditioned Medium ◽  
Hair Regeneration ◽  
Adipose Derived Stem Cell ◽  
Cell Conditioned Medium

scholarly journals Adipose-derived stem cell conditioned medium accelerates keratinocyte differentiation via the upregulation of miR-24

2015 ◽  
Vol 24 (10) ◽  
pp. 792-793 ◽  
Author(s):  
Gimoon Seo ◽  
Eunhye Oh ◽  
Mihee Yun ◽  
Ji-Young Lee ◽  
Joon Sung Bae ◽  
...  
Keyword(s):  
Stem Cell ◽  
Conditioned Medium ◽  
Keratinocyte Differentiation ◽  
Adipose Derived Stem Cell ◽  
Cell Conditioned Medium

scholarly journals Resveratrol Inhibits Alpha-Melanocyte-Stimulating Hormone Signaling, Viability, and Invasiveness in Melanoma Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Yu-Jen Chen ◽  
Ying-Yin Chen ◽  
Yi-Feng Lin ◽  
Hsuan-Yun Hu ◽  
Hui-Fen Liao
Keyword(s):  
Stem Cell ◽  
Cancer Stem Cell ◽  
Nuclear Translocation ◽  
Combined Treatment ◽  
Treatment Resistance ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Melanocyte Stimulating Hormone ◽  
B16 Melanoma Cells ◽  
Stimulating Hormone

Melanoma is a malignancy with high potential to invasion and treatment resistance. Theα-melanocyte-stimulating hormone (α-MSH) signal transduction involving Wnt/β-catenin, c-Kit, and microphthalmia-associated transcription factor (MITF), a known pathway to produce melanin, has been demonstrated as one of cancer stem cell characteristics. This study was aimed to examine the effect of resveratrol, an abundant ingredient of grape and medicinal plants, onα-MSH signaling, viability, and invasiveness in melanoma cells. Byα-MSH treatment, the melanin production in B16 melanoma cells was augmented as a validation for activation ofα-MSH signaling. The upregulated expression ofα-MSH signaling-related moleculesβ-catenin, c-Kit, and MITF was suppressed by resveratrol and/or STI571 treatment. Nuclear translocation of MITF, a hallmark ofα-MSH signaling activation, was inhibited by combined treatment of resveratrol and STI571. At effective concentration, resveratrol and/or STI571 inhibited cell viability andα-MSH-activated matrix metalloproteinase- (MMP-)9 expression and invasion capacity of B16 melanoma cells. In conclusion, resveratrol enhances STI571 effect on suppressing theα-MSH signaling, viability, and invasiveness in melanoma cells. It implicates that resveratrol may have potential to modulate the cancer stem cell characteristics of melanoma.

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