scholarly journals Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells

2015 ◽  
Vol 9 (4) ◽  
pp. 1641-1646 ◽  
Author(s):  
GA RIM KIM ◽  
GA-HEE HA ◽  
JAE-HO BAE ◽  
SAE-OCK OH ◽  
SUN-HEE KIM ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Natural Killer Cell ◽  
Cancer Cells ◽  
Killer Cell ◽  
Colon Cancer Cell ◽  
Cell Populations ◽  
Metastatic Colon Cancer ◽  
Colon Cancer Cells ◽  
Primary Colon Cancer ◽  
Primary Colon

Xanthohumol reduces inflammation and cell metabolism in HT29 primary colon cancer cells

2021 ◽  
pp. 1-9
Author(s):  
Margalida Torrens-Mas ◽  
Marina Alorda-Clara ◽  
Maria Martínez-Vigara ◽  
Pilar Roca ◽  
Jorge Sastre-Serra ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Cell Metabolism ◽  
Colon Cancer Cells ◽  
Primary Colon Cancer ◽  
Primary Colon

Abstract 252: Molecular characterization of cancer stem cells isolated from primary colon cancer cells.

2013 ◽  
Author(s):  
Martin Lange ◽  
Dirk Schumacher ◽  
Thomas Hauling ◽  
Christian Regenbrecht ◽  
Oliver Politz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Colon Cancer ◽  
Cancer Stem Cells ◽  
Cancer Cells ◽  
Molecular Characterization ◽  
Colon Cancer Cells ◽  
Primary Colon Cancer ◽  
Primary Colon

scholarly journals Identification of histone methyltransferase NSD2 as an important oncogenic gene in colorectal cancer

2021 ◽  
Vol 12 (11) ◽  
Author(s):  
Li-hao Zhao ◽  
Quan Li ◽  
Zhi-Jun Huang ◽  
Mi-Xue Sun ◽  
Jing-jing Lu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Cell Proliferation ◽  
Cancer Cells ◽  
Histone Methyltransferase ◽  
Migration And Invasion ◽  
Colon Cancer Cells ◽  
Akt Activation ◽  
Primary Colon Cancer ◽  
Primary Colon

AbstractColorectal cancer (CRC) is the second common cause of cancer-related human mortalities. Dysregulation of histone 3 (H3) methylation could lead to transcriptional activation of multiple oncogenes, which is closely associated with CRC tumorigenesis and progression. Nuclear receptor-binding SET Domain protein 2 (NSD2) is a key histone methyltransferase catalyzing histone H3 lysine 36 dimethylation (H3K36me2). Its expression, the potential functions, and molecular mechanisms in CRC are studied here. Gene Expression Profiling Interactive Analysis (GEPIA) bioinformatics results showed that the NSD2 mRNA expression is elevated in both colon cancers and rectal cancers. Furthermore, NSD2 mRNA and protein expression levels in local colon cancer tissues are significantly higher than those in matched surrounding normal tissues. In primary human colon cancer cells and established CRC cell lines, shRNA-induced silencing or CRISPR/Cas9-induced knockout of NSD2 inhibited cell viability, proliferation, cell cycle progression, migration, and invasion. Furthermore, NSD2 shRNA or knockout induced mitochondrial depolarization, DNA damage, and apoptosis in the primary and established CRC cells. Contrarily, ectopic NSD2 overexpression in primary colon cancer cells further enhanced cell proliferation, migration, and invasion. H3K36me2, expressions of multiple oncogenes (ADAM9, EGFR, Sox2, Bcl-2, SYK, and MET) and Akt activation were significantly decreased after NSD2 silencing or knockout in primary colon cancer cells. Their levels were however increased after ectopic NSD2 overexpression. A catalytic inactive NSD2 (Y1179A) also inhibited H3K36me2, multiple oncogenes expression, and Akt activation, as well as cell proliferation and migration in primary colon cancer cells. In vivo, intratumoral injection of adeno-associated virus (AAV)-packed NSD2 shRNA largely inhibited primary colon cancer cell xenograft growth in nude mice. Together, NSD2 exerted oncogenic functions in CRC and could be a promising therapeutic target.

Response of Primary Colon Cancer Cells in Hybrid Spheroids to 5-Fluorouracil

1993 ◽  
Vol 11 (3) ◽  
pp. 291-298 ◽  
Author(s):  
Bozidar Djordjevic ◽  
Christopher S. Lange ◽  
Ronald R. Allison ◽  
Marvin Rotman
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
Primary Colon Cancer ◽  
Primary Colon

scholarly journals Activation of Akt and MAPK pathways enhances the tumorigenicity of CD133+ primary colon cancer cells

2010 ◽  
Vol 31 (8) ◽  
pp. 1376-1380 ◽  
Author(s):  
Y. K. Wang ◽  
Y. L. Zhu ◽  
F. M. Qiu ◽  
T. Zhang ◽  
Z. G. Chen ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
Mapk Pathways ◽  
Primary Colon Cancer ◽  
Primary Colon

Colon cancer cells maintain low levels of pyruvate to avoid cell death caused by inhibition of HDAC1/HDAC3

2008 ◽  
Vol 417 (1) ◽  
pp. 379-389 ◽  
Author(s):  
Muthusamy Thangaraju ◽  
Kristina N. Carswell ◽  
Puttur D. Prasad ◽  
Vadivel Ganapathy
Keyword(s):  
Colon Cancer ◽  
Cell Death ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Gene Coding ◽  
Primary Colon Cancer ◽  
Primary Colon ◽  
Low Levels

Human colon cancer cells and primary colon cancer silence the gene coding for LDH (lactate dehydrogenase)-B and up-regulate the gene coding for LDH-A, resulting in effective conversion of pyruvate into lactate. This is associated with markedly reduced levels of pyruvate in cancer cells compared with non-malignant cells. The silencing of LDH-B in cancer cells occurs via DNA methylation, with involvement of the DNMTs (DNA methyltransferases) DNMT1 and DNMT3b. Colon cancer is also associated with the expression of pyruvate kinase M2, a splice variant with low catalytic activity. We have shown recently that pyruvate is an inhibitor of HDACs (histone deacetylases). Here we show that pyruvate is a specific inhibitor of HDAC1 and HDAC3. Lactate has no effect on any of the HDACs examined. Colon cancer cells exhibit increased HDAC activity compared with non-malignant cells. HDAC1 and HDAC3 are up-regulated in colon cancer cells and in primary colon cancer, and siRNA (small interfering RNA)-mediated silencing of HDAC1 and HDAC3 in colon cancer cells induces apoptosis. Colon cancer cells silence SLC5A8, the gene coding for a Na+-coupled pyruvate transporter. Heterologous expression of SLC5A8 in the human colon cancer cell line SW480 leads to inhibition of HDAC activity when cultured in the presence of pyruvate. This process is associated with an increase in intracellular levels of pyruvate, increase in the acetylation status of histone H4, and enhanced cell death. These studies show that cancer cells effectively maintain low levels of pyruvate to prevent inhibition of HDAC1/HDAC3 and thereby to evade cell death.

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