scholarly journals Homologous mesenchymal stem cells promote the emergence and growth of pulmonary metastases of the rat osteosarcoma cell line UMR-106

2014 ◽  
Vol 8 (1) ◽  
pp. 127-132 ◽  
Author(s):  
PENG ZHANG ◽  
LING DONG ◽  
HUA LONG ◽  
TONG-TAO YANG ◽  
YONG ZHOU ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Line ◽  
Pulmonary Metastases ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Umr 106

Conditioned medium of the osteosarcoma cell line U2OS induces hBMSCs to exhibit characteristics of carcinoma-associated fibroblasts via activation of IL-6/STAT3 signalling

2020 ◽  
Vol 168 (3) ◽  
pp. 265-271 ◽  
Author(s):  
Longshuai Lin ◽  
Kai Huang ◽  
Weihong Guo ◽  
Chenghao Zhou ◽  
Gangyang Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Line ◽  
Conditioned Medium ◽  
Osteosarcoma Cell Line ◽  
Migration And Invasion ◽  
Osteosarcoma Cell ◽  
Bone Mesenchymal Stem Cells ◽  
Stat3 Signalling ◽  
Carcinoma Associated Fibroblasts

Abstract As a research hotspot in recent years, bone mesenchymal stem cells (BMSCs) play an important role in the process of a variety of human diseases, including cancers. However, in osteosarcoma, the role of BMSCs and their communication with tumour cells are not clear. In this study, we validated the communication of osteosarcoma (OS) cells with BMSCs. The results showed that the conditioned medium of osteosarcoma cell line U2OS (U2OS-CM) induces the carcinoma-associated fibroblasts (CAFs)-like transformation of BMSCs and promotes the proliferation, migration and invasion of BMSCs. Mechanistically, treatment of human bone mesenchymal stem cells (hBMSCs) with U2OS-CM results in a significant increase in the IL-6 expression and phosphorylation of STAT3. Furthermore, blockade of the IL-6/STAT3 signalling in hBMSCs rescues the transformation of CAF phenotype induced by U2OS-CM. And, human IL-6 can directly increase the expression of the CAF marker genes in hMSCs. Meanwhile, IL-6/STAT3 signalling involves in promoting effects of U2OS-CM on the proliferation, migration and invasion of BMSCs. In summary, our results suggest that BMSCs communicate with OS cells through IL-6/STAT3 signalling and play an important role in the progress of osteosarcoma.

Uncoupling Warburg effect and stemness in CD133+ve cancer stem cells from Saos-2 (osteosarcoma) cell line under hypoxia

2018 ◽  
Vol 45 (6) ◽  
pp. 1653-1662 ◽  
Author(s):  
Pavani Koka ◽  
Reddy Sailaja Mundre ◽  
Rohini Rangarajan ◽  
Yamini Chandramohan ◽  
Raghunandha Kumar Subramanian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cell Line ◽  
Warburg Effect ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell

Effects of parathyroid hormone on cyclic AMP-formation and cytoplasmic free Ca2+ in the osteosarcoma cell line UMR 106-01

1992 ◽  
Vol 12 (3) ◽  
pp. 207-214 ◽  
Author(s):  
Östen Ljunggren ◽  
Hans Johansson ◽  
Ulf H. Lerner ◽  
Erik Lindh ◽  
Sverker Ljunghall
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Cell Line ◽  
Single Cells ◽  
Transient Increase ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Fura 2 ◽  
Umr 106 ◽  
Camp Formation

The effects of parathyroid hormone (PTH) on cytoplasmic free CA2+ (Cai2+) and cAMP-formation were investigated in the rat osteosarcoma cell line UMR 106-01. In fura-2 loaded adherent single cells bPTH 1–34 (10 nM−1μM) induced a rapid transient increase in Cai2+ in 11% of the studied cells. In fura-2 tracings from UMR 106-01 cells in suspension, bPTH 1–34 (0.1 μM) induced a transient increase in Cai2+ in 20% of the experiments. The transient increase in Cai2+ seen in suspensions of cells was not abolished by addition of EGTA (2.5 mM) prior to challenge with PTH, suggesting that the increase in Cai2+ was derived from intracellular stores. A marked rapid increase in cAMP-formation was observed in all experiments with cells in suspension, also in the experiments where PTH did not affect Cai2+. These data show that PTH causes a release of Ca2+ from intracellular stores in a small percentage of osteosarcoma UMR 106-01 cells, and that PTH is capable of inducing an increase in cAMP-formation without affecting Cai2+ in osteoblasts.

scholarly journals Characterization of volume-sensitive, calcium-permeating pathways in the osteosarcoma cell line UMR-106-01

1989 ◽  
Vol 264 (8) ◽  
pp. 4383-4390 ◽  
Author(s):  
D T Yamaguchi ◽  
J Green ◽  
C R Kleeman ◽  
S Muallem
Keyword(s):  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Umr 106
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