scholarly journals Combination of four gene markers to detect circulating tumor cells in the peripheral blood of patients with advanced lung adenocarcinoma using real-time PCR

2013 ◽  
Vol 5 (4) ◽  
pp. 1400-1406 ◽  
Author(s):  
YAN YU ◽  
GANG XU ◽  
JINGYAN CAO ◽  
SHI JIN ◽  
YINGCHUN MAN ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Real Time ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Real Time Pcr ◽  
Peripheral Blood ◽  
Gene Markers

scholarly journals Molecular characterization of circulating tumor cells in large quantities of contaminating leukocytes by a multiplex real-time PCR

2008 ◽  
Vol 118 (3) ◽  
pp. 455-468 ◽  
Author(s):  
Anieta M. Sieuwerts ◽  
Jaco Kraan ◽  
Joan Bolt-de Vries ◽  
Petra van der Spoel ◽  
Bianca Mostert ◽  
...  
Keyword(s):  
Real Time ◽  
Tumor Cells ◽  
Molecular Characterization ◽  
Circulating Tumor Cells ◽  
Real Time Pcr

Survivin expression in circulating tumor cells of patients with gastric cancer.

2014 ◽  
Vol 32 (3_suppl) ◽  
pp. 65-65
Author(s):  
Masanori Terashima ◽  
Keiichi Hatakeyama ◽  
Yushi Yamakawa ◽  
Norihiko Sugisawa ◽  
Yuichiro Miki ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Mrna Expression ◽  
Real Time ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Peripheral Blood ◽  
Survivin Expression ◽  
Expression Level ◽  
Survivin Mrna ◽  
Pcr Targeting

65 Background: Detection of circulating tumor cells (CTC) in peripheral blood is thought to be a promising method to evaluate the extent of minimal residual disease that may be useful for predicting recurrence and for monitoring treatment efficacy. However, unlike breast or lung cancer, little information is available in patients with gastric cancer. Among them, evaluation of survivin, a member of the inhibitor of apoptosis (IAP) family, mRNA expression in peripheral blood appears to be a promising procedure with relative good performance for predicting recurrence after curative resection. In order to evaluate the clinical significance of surviving expression in peripheral blood, we performed real-time RT-PCR analysis using whole blood obtained from patients with gastric cancer. Methods: A total of 95 patients with gastric cancer treated at our cancer center between Nov. 2009 and Aug. 2012 were enrolled in this study. Peripheral blood samples were collected using PAX gene and RNA was isolated using Boom method. After cDNA synthesis, quantitative real-time PCR targeting survivin gene was performed. The relative expression level of survivin mRNA was calculated with actin beta as an internal standard. Correlation between survivin expression and clinicopathologic factors was investigated. Results: Survivin mRNA expression level in peripheral blood ranged from 34.8 to 1889.6, and the median level was 370.3. Survivin mRNA level tended to be higher in patients with T4, N3b, CY1, and Stage IV. There was no significant correlation with histological type, lympho-vascular invasion, or macroscopic type. If we set a cut-off value at 540, survivin was positive in 27 patients (28%). Disease-free survival was significantly worse in survivin positive patients than in negative patients (p=0.0216). Conclusions: Survivin expression in CTC was higher in advanced stage and appears to be a good prognostic marker in patients with gastric cancer. Validation with large sample size should be performed to confirm the significance of survivin in CTC.

Detection of Circulating Tumor Cells in Peripheral Blood of Breast Cancer Patients During or After Therapy Using a Multigene Real-Time RT-PCR Assay

2006 ◽  
Vol 10 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Barbara K. Zehentner ◽  
Heather Secrist ◽  
Dawn C. Hayes ◽  
Xinqun Zhang ◽  
Richard C. Ostenson ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Real Time ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Peripheral Blood ◽  
Breast Cancer Patients ◽  
Rt Pcr ◽  
Pcr Assay
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