scholarly journals 8-Chloroadenosine 3′,5′-monophosphate induces cell cycle arrest and apoptosis in multiple myeloma cells through multiple mechanisms

2012 ◽  
Vol 4 (6) ◽  
pp. 1384-1388 ◽  
Author(s):  
YI-MIN CHENG ◽  
QI ZHU ◽  
YI-YUN YAO ◽  
YONG TANG ◽  
MING-MING WANG ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Multiple Myeloma ◽  
Cell Cycle Arrest ◽  
Myeloma Cells ◽  
Cycle Arrest

scholarly journals The insulin-like growth factor-I receptor inhibitor NVP-AEW541 provokes cell cycle arrest and apoptosis in multiple myeloma cells

2008 ◽  
Vol 141 (4) ◽  
pp. 470-482 ◽  
Author(s):  
Patricia Maiso ◽  
Enrique M. Ocio ◽  
Mercedes Garayoa ◽  
Juan C. Montero ◽  
Francesco Hofmann ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Multiple Myeloma ◽  
Growth Factor ◽  
Cell Cycle Arrest ◽  
Insulin Like Growth Factor ◽  
Myeloma Cells ◽  
Factor I ◽  
Cycle Arrest ◽  
Growth Factor I ◽  
Receptor Inhibitor

Bone morphogenetic protein-2 induces apoptosis in human myeloma cells with modulation of STAT3

Blood ◽  
2000 ◽  
Vol 96 (6) ◽  
pp. 2005-2011 ◽  
Author(s):  
Chiharu Kawamura ◽  
Masahiro Kizaki ◽  
Kenji Yamato ◽  
Hideo Uchida ◽  
Yumi Fukuchi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Multiple Myeloma ◽  
Cell Cycle Arrest ◽  
Transforming Growth Factor ◽  
Bone Morphogenetic Protein 2 ◽  
Bone Lesions ◽  
Myeloma Cells ◽  
Autocrine Loop ◽  
Cycle Arrest ◽  
Human Myeloma Cells

Abstract Bone morphogenetic proteins (BMPs), members of the transforming growth factor (TGF)–β superfamily, are a group of related proteins that are capable of inducing the formation of cartilage and bone but are now regarded as multifunctional cytokines. We show in this report a novel function of BMPs in hematopoietic cells: BMP-2 induces apoptosis not only in human myeloma cell lines (U266, RPMI 8226, HS-Sultan, IM-9, OPM-2, and KMS-12 cells), but also in primary samples from patients with multiple myeloma. The mechanism of BMP-2–induced apoptosis was investigated with the use of U266 cells, which are dependent on the interleukin-6 autocrine loop. We showed that BMP-2 caused cell-cycle arrest in the G1 phase and the subsequent apoptosis of myeloma cells. BMP-2 up-regulated the expression of cyclin-dependent kinase inhibitors (p21CIP1/WAF1 and p27KIP1) and caused hypophosphorylation of retinoblastoma (Rb) protein. In studies of apoptosis-associated proteins, BMP-2 was seen to down-regulate the expression of Bcl-xL; however, BMP-2 had no effects on the expression of Bcl-2, Bax, or Bad. Therefore, BMP-2 induces apoptosis in various human myeloma cells by means of the down-regulation of Bcl-xL and by cell-cycle arrest through the up-regulation of p21CIP1/WAF1 and p27KIP1 and by the hypophosphorylation of Rb. Further analysis showed that the signal transducer and activator of transcription 3 (STAT3) was inactivated immediately after BMP-2 treatment. We conclude that BMP-2 would be useful as a novel therapeutic agent in the treatment of multiple myeloma both by means of its antitumor effect of inducing apoptotis and through its original bone-inducing activity, because bone lesions are frequently seen in myeloma patients.

Bone morphogenetic protein-2 induces apoptosis in human myeloma cells with modulation of STAT3

Blood ◽  
2000 ◽  
Vol 96 (6) ◽  
pp. 2005-2011 ◽  
Author(s):  
Chiharu Kawamura ◽  
Masahiro Kizaki ◽  
Kenji Yamato ◽  
Hideo Uchida ◽  
Yumi Fukuchi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Multiple Myeloma ◽  
Cell Cycle Arrest ◽  
Transforming Growth Factor ◽  
Bone Morphogenetic Protein 2 ◽  
Bone Lesions ◽  
Myeloma Cells ◽  
Autocrine Loop ◽  
Cycle Arrest ◽  
Human Myeloma Cells

Bone morphogenetic proteins (BMPs), members of the transforming growth factor (TGF)–β superfamily, are a group of related proteins that are capable of inducing the formation of cartilage and bone but are now regarded as multifunctional cytokines. We show in this report a novel function of BMPs in hematopoietic cells: BMP-2 induces apoptosis not only in human myeloma cell lines (U266, RPMI 8226, HS-Sultan, IM-9, OPM-2, and KMS-12 cells), but also in primary samples from patients with multiple myeloma. The mechanism of BMP-2–induced apoptosis was investigated with the use of U266 cells, which are dependent on the interleukin-6 autocrine loop. We showed that BMP-2 caused cell-cycle arrest in the G1 phase and the subsequent apoptosis of myeloma cells. BMP-2 up-regulated the expression of cyclin-dependent kinase inhibitors (p21CIP1/WAF1 and p27KIP1) and caused hypophosphorylation of retinoblastoma (Rb) protein. In studies of apoptosis-associated proteins, BMP-2 was seen to down-regulate the expression of Bcl-xL; however, BMP-2 had no effects on the expression of Bcl-2, Bax, or Bad. Therefore, BMP-2 induces apoptosis in various human myeloma cells by means of the down-regulation of Bcl-xL and by cell-cycle arrest through the up-regulation of p21CIP1/WAF1 and p27KIP1 and by the hypophosphorylation of Rb. Further analysis showed that the signal transducer and activator of transcription 3 (STAT3) was inactivated immediately after BMP-2 treatment. We conclude that BMP-2 would be useful as a novel therapeutic agent in the treatment of multiple myeloma both by means of its antitumor effect of inducing apoptotis and through its original bone-inducing activity, because bone lesions are frequently seen in myeloma patients.

scholarly journals The hypoxia-mimetic agent cobalt chloride induces cell cycle arrest and alters gene expression in U266 multiple myeloma cells

2012 ◽  
Vol 30 (5) ◽  
pp. 1180-1186 ◽  
Author(s):  
SEUNGHEE BAE ◽  
HYE-JUNG JEONG ◽  
HWA JUN CHA ◽  
KARAM KIM ◽  
YEONG MIN CHOI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Multiple Myeloma ◽  
Cell Cycle Arrest ◽  
Cobalt Chloride ◽  
Myeloma Cells ◽  
Cycle Arrest

Aptamer TY04 inhibits the growth of multiple myeloma cells via cell cycle arrest

Tumor Biology ◽  
2014 ◽  
Vol 35 (8) ◽  
pp. 7561-7568 ◽  
Author(s):  
Hongjuan Dai ◽  
Mao Ye ◽  
Mingyuan Peng ◽  
Weihua Zhou ◽  
Huarong Bai ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Multiple Myeloma ◽  
Cell Cycle Arrest ◽  
Myeloma Cells ◽  
Cycle Arrest

Involvement of Chk1–Cdc25A-cyclin A/CDk2 pathway in simvastatin induced S-phase cell cycle arrest and apoptosis in multiple myeloma cells

2011 ◽  
Vol 670 (2-3) ◽  
pp. 356-364 ◽  
Author(s):  
Yong-Sheng Tu ◽  
Xiao-Long Kang ◽  
Jia-Guo Zhou ◽  
Xiao-Fei Lv ◽  
Yong-Bo Tang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Multiple Myeloma ◽  
Cell Cycle Arrest ◽  
Cyclin A ◽  
S Phase ◽  
Phase Cell ◽  
Myeloma Cells ◽  
Cycle Arrest
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