scholarly journals In vitro gastric cancer cell imaging using near-infrared quantum dot-conjugated CC49

2012 ◽  
Vol 4 (5) ◽  
pp. 996-1002 ◽  
Author(s):  
YUN-PENG ZHANG ◽  
PENG SUN ◽  
XU-RUI ZHANG ◽  
WU-LI YANG
Keyword(s):  
Gastric Cancer ◽  
Quantum Dot ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Near Infrared ◽  
Cell Imaging ◽  
Cancer Cell Imaging

Single-chain Antibody Against Reg4 Suppresses Gastric Cancer Cell Growth and Enhances 5-FU-induced Cell Death in vitro

2019 ◽  
Vol 19 (5) ◽  
pp. 610-619 ◽  
Author(s):  
Xue-Qing Zhang ◽  
Lu-Ting Yu ◽  
Pei Du ◽  
Tian-Qi Yin ◽  
Zhi-Yuan Zhang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Cell Death ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Ags Cells ◽  
Thiazolyl Blue Tetrazolium Bromide

Background:Regenerating islet-derived gene family member 4 (Reg4), a well-investigated growth factor in the regenerative pancreas, has recently been reported to be highly associated with a majority of gastrointestinal cancers. Pathological hyper-expression or artificial over-expression of Reg4 causes acceleration of tumor growth, migration, and resistance to chemotherapeutic 5-Fluorouracil (5-FU). Until now, no method has been successfully established for eliminating the effects of Reg4 protein.Methods:This study reports the production of an engineered immunoglobin, a single-chain variable fragment (scFv-Reg4), to specifically bind Reg4 and block the bioactivity. The complementary-determining regions (CDRs) against Reg4 were assigned using MOE and ZDOCK servers. The binding affinity (KD) was determined by bio-layer interferometry (BLI). MKN45 and AGS cell proliferation was determined by Thiazolyl blue tetrazolium bromide (MTT) method and the cell apoptosis was detected by flow cytometry assay.Results:The KD of scFv-Reg4 to Reg4 was determined to be 1.91×10-8. In MKN45 and AGS cell lines, scFv- Reg4 depressed Reg4-stimulated cell proliferation and the inhibitory rates were 27.7±1.5% and 17.3±2.6%, respectively. Furthermore, scFv significantly enhanced 5-FU-induced cell death, from 23.0±1.0% to 28.4±1.2% in MKN45 and 28.2±0.7% to 36.6±0.6% in AGS cells. Treatment with scFv alone could lyse cancer cells to a certain extent, but no significance has been observed.Conclusion:The single-chain antibody (scFv-Reg4) significantly inhibited gastric cancer cell proliferation and synergistically enhanced the lethal effect of 5-FU. Thus, traditional chemo-/radio- therapeutics supplemented with scFv-Reg4 may provide advances in the strategy for gastrointestinal cancer treatment.

KLa(0.95−x)GdxF4:Eu3+ hexagonal phase nanoparticles as luminescent probes for in vitro Huh-7 cancer cell imaging

2021 ◽  
Vol 50 (15) ◽  
pp. 5197-5207
Author(s):  
Mohini Gupta ◽  
Rajamani Nagarajan ◽  
Chitteti Ramamurthy ◽  
Perumal Vivekanandan ◽  
G. Vijaya Prakash
Keyword(s):  
Liver Cancer ◽  
Cancer Cell ◽  
Cell Imaging ◽  
Hexagonal Phase ◽  
Liver Cancer Cell ◽  
Luminescent Probes ◽  
Cancer Cell Imaging ◽  
Site Selective

Strong and site selective red-emitting photoluminescent/MRI multi-functional KLa(0.95−x)GdxF4:Eu3+ (x = 0–0.4) bio-compatible nanomaterials for targeted in-vitro liver cancer cell imaging.

Characterization of a near-infrared fluorescent DCPO-tagged glucose analogue for cancer cell imaging

2017 ◽  
Vol 166 ◽  
pp. 264-271 ◽  
Author(s):  
Yanfen Fang ◽  
Wanli Zhang ◽  
Mengli Zhu ◽  
Shiguang Chen ◽  
Xuan Liu ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Near Infrared ◽  
Cell Imaging ◽  
Cancer Cell Imaging ◽  
Glucose Analogue

Highly efficient near-infrared organic dots based on novel AEE fluorogen for specific cancer cell imaging

RSC Advances ◽  
2015 ◽  
Vol 5 (46) ◽  
pp. 36837-36844 ◽  
Author(s):  
Yan Zhang ◽  
Kaiwen Chang ◽  
Bin Xu ◽  
Jinlong Chen ◽  
Lulin Yan ◽  
...  
Keyword(s):  
Quantum Efficiency ◽  
Cancer Cell ◽  
Small Molecule ◽  
Near Infrared ◽  
Cell Imaging ◽  
Amphiphilic Polymer ◽  
Cancer Cell Imaging ◽  
Highly Efficient ◽  
Specific Cancer ◽  
High Fluorescence

Near-infrared emissive organic dots with a high fluorescence quantum efficiency (AEE dots) are prepared by using an amphiphilic polymer PSMA and a novel small molecule fluorogen (DPPBPA).

scholarly journals Targeting Nuclear Exporter Protein XPO1/CRM1 in Gastric Cancer

2019 ◽  
Vol 20 (19) ◽  
pp. 4826 ◽  
Author(s):  
Rachel Sexton ◽  
Zaid Mahdi ◽  
Rahman Chaudhury ◽  
Rafic Beydoun ◽  
Amro Aboukameel ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Nuclear Export ◽  
Gastric Cancer Cell ◽  
Clinical Investigation ◽  
Chromosome Region ◽  
Specific Inhibitor ◽  
Anti Tumor Activity

Gastric cancer remains an unmet clinical problem in urgent need of newer and effective treatments. Here we show that the nuclear export protein, Exportin 1 (XPO1, chromosome region maintenance 1 or CRM1), is a promising molecular target in gastric cancer. We demonstrate significant overexpression of XPO1 in a cohort of histologically diverse gastric cancer patients with primary and metastatic disease. XPO1 RNA interference suppressed gastric cancer cell growth. Anti-tumor activity was observed with specific inhibitor of nuclear export (SINE) compounds (selinexor/XPOVIO), second-generation compound KPT-8602/eltanexor, KPT-185 and +ve control Leptomycin B in three distinct gastric cancer cell lines. SINE compounds inhibited gastric cancer cell proliferation, disrupted spheroid formation, induced apoptosis and halted cell cycle progression at the G1/S phase. Anti-tumor activity was concurrent with nuclear retention of tumor suppressor proteins and inhibition of colony formation. In combination studies, SINE compounds enhanced the efficacy of nab-paclitaxel in vitro and in vivo. More significantly, using non-coding RNA sequencing studies, we demonstrate for the first time that SINE compounds can alter the expression of non-coding RNAs (microRNAs and piwiRNAs). SINE treatment caused statistically significant downregulation of oncogenic miR-33b-3p in two distinct cell lines. These studies demonstrate the therapeutic significance of XPO1 in gastric cancer that warrants further clinical investigation.

Corrigendum to “Fluorescent carbon nanodots for targeted in vitro cancer cell imaging” [Appl. Mater. Today 4 (2016) 71–77]

2019 ◽  
Vol 17 ◽  
pp. 236-240 ◽  
Author(s):  
Sandhya Aiyer ◽  
Rajendra Prasad ◽  
Manoj Kumar ◽  
K. Nirvikar ◽  
Bhanprakash Jain ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Cell Imaging ◽  
Carbon Nanodots ◽  
Cancer Cell Imaging ◽  
Fluorescent Carbon Nanodots ◽  
Fluorescent Carbon
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