scholarly journals Silencing of anti-apoptotic transmembrane protein lifeguard sensitizes solid tumor cell lines MCF-7 and SW872 to perifosine-induced cell death activation

2011 ◽  
Vol 2 (3) ◽  
pp. 419-422 ◽  
Author(s):  
VESNA BUCAN ◽  
CLAUDIA Y.U. CHOI ◽  
ANDREA LAZARIDIS ◽  
PETER M. VOGT ◽  
KERSTIN REIMERS
Keyword(s):  
Cell Death ◽  
Tumor Cell ◽  
Cell Lines ◽  
Solid Tumor ◽  
Transmembrane Protein ◽  
Tumor Cell Lines ◽  
Mcf 7

Different cell death responses induced by eupomatenoid-5 in MCF-7 and 786-0 tumor cell lines

2015 ◽  
Vol 29 (5) ◽  
pp. 1026-1033 ◽  
Author(s):  
Giovanna Barbarini Longato ◽  
Giovanna Francisco Fiorito ◽  
Débora Barbosa Vendramini-Costa ◽  
Ilza Maria de Oliveira Sousa ◽  
Sirlene Valério Tinti ◽  
...  
Keyword(s):  
Cell Death ◽  
Tumor Cell ◽  
Cell Lines ◽  
Tumor Cell Lines ◽  
Mcf 7

Triptolide Exerts a Cytotoxic Effect on Human Acute Myeloid Leukemia Cell Lines

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 4352-4352
Author(s):  
Jennifer M.H. McCormack ◽  
Tanya Feldberg ◽  
Jatinder Lamba
Keyword(s):  
Cell Death ◽  
Acute Myeloid Leukemia ◽  
Tumor Cell ◽  
Cell Lines ◽  
Solid Tumor ◽  
Myeloid Leukemia ◽  
Cytotoxic Effect ◽  
Single Agent ◽  
Tumor Cell Lines ◽  
Acute Myeloid

Abstract Abstract 4352 Background: Triptolide, a diterpenoid derived from the Chinese herb Tripterygium wilfordii Hook f., has shown anti-tumor effects in various leukemia and solid tumor cell lines in vitro. Previous studies have shown that triptolide synergizes with various chemotherapeutic agents in both leukemia and solid tumor cell lines. Triptolide has been shown to synergize with cytarabine in the THP-1 acute myeloid leukemia (AML) cell line (Pigneux et al 2008). This is of particular interest because cytarabine forms the backbone of AML therapy in both children and adults, and cytarabine resistance poses a significant challenge in the setting of refractory or relapsed AML. Therefore, an agent that works synergistically with cytarabine is of particular interest as a potential new agent for the treatment of relapsed or refractory AML. The aim of this study was to assess the cytotoxicity of triptolide, both as a single agent and in combination with cytarabine, in human AML cell lines representing low-, intermediate-, and high-relapse risk groups. Methods: The following cell lines were selected: Kasumi-1 (low risk, t(8;21)), HL-60 and THP-1 (intermediate risk), and MV4-11 (high risk, FLT3 ITD). Kasumi-1 and HL-60 cells were cultured in RPMI 1640 media with 20% fetal bovine serum (FBS). MV4-11 cells were cultured in IMDM media with 10% FBS and THP-1 cells were cultured in RPMI with 10% FBS. We performed the following drug treatments: triptolide alone; cytarabine alone; co-treatment with triptolide and cytarabine. Drug-induced cytotoxicity after 48 hours of treatment was assessed using the MTT assay. The combination index (CI) was determined using Calcusyn software (Biosoft, Cambridge, UK). Results: Single-agent triptolide induced cell death in all human AML cell lines tested, including cytarabine-resistant Kasumi-1 cells, at low nanomolar concentrations (average Ic50 ranging from 6–15 nM). The combination of triptolide and cytarabine acted synergistically to induce cell death in THP-1 cells as has been previously reported. Interestingly, the combination of triptolide and cytarabine acted in an antagonistic fashion in all other cell lines tested, except for at low drug concentrations in MV4-11 cells. Conclusion: Single-agent triptolide exerts a cytotoxic effect on human AML cell lines, including those that are relatively resistant to cytarbine. The effect of the combination of triptolide and cytarabine was cell-line dependent. The results of this study warrant further investigation on the potential role of triptolide as an anti-leukemic agent in combination with cytarabine. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Adenovirus encoding HIV-1 Vpr activates caspase 9 and induces apoptotic cell death in both p53 positive and negative human tumor cell lines

Oncogene ◽  
2002 ◽  
Vol 21 (30) ◽  
pp. 4613-4625 ◽  
Author(s):  
Karuppiah Muthumani ◽  
Donghui Zhang ◽  
Daniel S Hwang ◽  
Sagar Kudchodkar ◽  
Nathanael S Dayes ◽  
...  
Keyword(s):  
Cell Death ◽  
Tumor Cell ◽  
Cell Lines ◽  
Apoptotic Cell ◽  
Human Tumor ◽  
Tumor Cell Lines ◽  
Caspase 9 ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines ◽  
Hiv 1

Correlation between Potassium Channel Expression and Sensitivity to Drug-induced Cell Death in Tumor Cell Lines

2014 ◽  
Vol 20 (2) ◽  
pp. 189-200 ◽  
Author(s):  
Luigi Leanza ◽  
Paul O’Reilly ◽  
Anne Doyle ◽  
Elisa Venturini ◽  
Mario Zoratti ◽  
...  
Keyword(s):  
Cell Death ◽  
Tumor Cell ◽  
Potassium Channel ◽  
Cell Lines ◽  
Tumor Cell Lines ◽  
Drug Induced ◽  
Channel Expression

C-prenylated flavonoids with potential cytotoxic activity against solid tumor cell lines

2019 ◽  
Vol 18 (4) ◽  
pp. 1051-1100
Author(s):  
Lenka Molčanová ◽  
Dominika Janošíková ◽  
Stefano Dall´Acqua ◽  
Karel Šmejkal
Keyword(s):  
Tumor Cell ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Solid Tumor ◽  
Tumor Cell Lines ◽  
Prenylated Flavonoids

scholarly journals GATA-1 and GATA-2 binding to 3′ enhancer of WT1 gene is essential for its transcription in acute leukemia and solid tumor cell lines

Leukemia ◽  
2009 ◽  
Vol 23 (7) ◽  
pp. 1270-1277 ◽  
Author(s):  
A Furuhata ◽  
M Murakami ◽  
H Ito ◽  
S Gao ◽  
K Yoshida ◽  
...  
Keyword(s):  
Acute Leukemia ◽  
Tumor Cell ◽  
Cell Lines ◽  
Solid Tumor ◽  
Tumor Cell Lines ◽  
Wt1 Gene

Cytochalasin P1, a new cytochalasin from the marine-derived fungus Xylaria sp. SOF11

2017 ◽  
Vol 72 (3-4) ◽  
pp. 129-132 ◽  
Author(s):  
Ziming Chen ◽  
Yuchan Chen ◽  
Hongbo Huang ◽  
Hongyan Yang ◽  
Weimin Zhang ◽  
...  
Keyword(s):  
South China Sea ◽  
Tumor Cell ◽  
Cell Lines ◽  
South China ◽  
The South China Sea ◽  
Tumor Cell Lines ◽  
The South ◽  
China Sea ◽  
Data Analyses ◽  
Mcf 7

Abstract A new cytochalasin, named cytochalasin P1 (1), together with four known analogs (2–5) was isolated from marine-derived fungus Xylaria sp. SOF11 from the South China Sea. The structure of the new compound was elucidated on the basis of MS and NMR (1H, 13C, HSQC, HMBC, and NOESY) data analyses. Compounds 1–5 were tested for their cytotoxicities against four tumor cell lines (SF-268, MCF-7, NCI-H460, and HepG-2). Compounds 1–5 showed significant cytotoxicity against two tumor cell lines MCF-7 and SF-268, with the IC50 values varying between 0.33 and 4.17 μM.

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