scholarly journals CTF18 interacts with replication protein A in response to replication stress

2016 ◽  
Vol 14 (1) ◽  
pp. 367-372 ◽  
Author(s):  
YUTA KANEKO ◽  
HIROAKI DAITOKU ◽  
CHIHIRO KOMENO ◽  
AKIYOSHI FUKAMIZU
Keyword(s):  
Protein A ◽  
Replication Stress ◽  
Replication Protein A ◽  
Replication Protein

scholarly journals Human DNA Helicase B (HDHB) Binds to Replication Protein A and Facilitates Cellular Recovery from Replication Stress

2011 ◽  
Vol 287 (9) ◽  
pp. 6469-6481 ◽  
Author(s):  
Gulfem Dilek Guler ◽  
Hanjian Liu ◽  
Sivaraja Vaithiyalingam ◽  
Diana R. Arnett ◽  
Elisabeth Kremmer ◽  
...  
Keyword(s):  
Protein A ◽  
Replication Stress ◽  
Replication Protein A ◽  
Dna Helicase ◽  
Replication Protein ◽  
Human Dna

scholarly journals E3 ligase RFWD3 is a novel modulator of stalled fork stability in BRCA2-deficient cells

2020 ◽  
Vol 219 (6) ◽  
Author(s):  
Haohui Duan ◽  
Sarah Mansour ◽  
Rachel Reed ◽  
Margaret K. Gillis ◽  
Benjamin Parent ◽  
...  
Keyword(s):  
Protein A ◽  
E3 Ligase ◽  
Replication Stress ◽  
Replication Protein A ◽  
Replication Protein ◽  
Genomic Integrity ◽  
Repair Pathway ◽  
Cell Sensitivity ◽  
Stalled Fork

BRCA1/2 help maintain genomic integrity by stabilizing stalled forks. Here, we identify the E3 ligase RFWD3 as an essential modulator of stalled fork stability in BRCA2-deficient cells and show that codepletion of RFWD3 rescues fork degradation, collapse, and cell sensitivity upon replication stress. Stalled forks in BRCA2-deficient cells accumulate phosphorylated and ubiquitinated replication protein A (ubq-pRPA), the latter of which is mediated by RFWD3. Generation of this intermediate requires SMARCAL1, suggesting that it depends on stalled fork reversal. We show that in BRCA2-deficient cells, rescuing fork degradation might not be sufficient to ensure fork repair. Depleting MRE11 in BRCA2-deficient cells does block fork degradation, but it does not prevent fork collapse and cell sensitivity in the presence of replication stress. No such ubq-pRPA intermediate is formed in BRCA1-deficient cells, and our results suggest that BRCA1 may function upstream of BRCA2 in the stalled fork repair pathway. Collectively, our data uncover a novel mechanism by which RFWD3 destabilizes forks in BRCA2-deficient cells.

scholarly journals Replication Protein A Availability during DNA Replication Stress Is a Major Determinant of Cisplatin Resistance in Ovarian Cancer Cells

2018 ◽  
Vol 78 (19) ◽  
pp. 5561-5573 ◽  
Author(s):  
François Bélanger ◽  
Emile Fortier ◽  
Maxime Dubé ◽  
Jean-François Lemay ◽  
Rémi Buisson ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Dna Replication ◽  
Cancer Cells ◽  
Cisplatin Resistance ◽  
Protein A ◽  
Replication Stress ◽  
Replication Protein A ◽  
Replication Protein ◽  
Ovarian Cancer Cells ◽  
Dna Replication Stress
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