scholarly journals Effects of bone marrow mesenchymal stem cells on the cardiac function and immune system of mice with endotoxemia

2016 ◽  
Vol 13 (6) ◽  
pp. 5317-5325 ◽  
Author(s):  
YUANFAN WU ◽  
JING ZHOU ◽  
LIQING BI ◽  
MIN HUANG ◽  
YI HAN ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Immune System ◽  
Cardiac Function ◽  
Marrow Mesenchymal Stem Cells

Impact of Bone Marrow Mesenchymal Stem Cells That Express Vascular Endothelial Growth Factor on Cardiac Function After Myocardial Infarction

2021 ◽  
Vol 11 (1) ◽  
pp. 44-50
Author(s):  
Yongming He ◽  
Ping Li ◽  
Yunlong Chen ◽  
Youmei Li
Keyword(s):  
Myocardial Infarction ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cardiac Function ◽  
Troponin T ◽  
Gene Transfection ◽  
Control Group ◽  
Marrow Mesenchymal Stem Cells ◽  
The Impact

Transplanted bone marrow mesenchymal stem cells (MSCs) can differentiate into cardiomyocytes and may have the potential to replace necrotic cardiomyocytes resulting from myocardial infarction (MI). Here we established a method for transfection of MSCs with an expression vector encoding human vascular Eedothelial Ggowth Ffctor (hVEGF). We evaluated the impact of transplantation of transfected MSCs on the recovery cardiac function and angiogenesis in a rat model of MI. Rat MSCs were separated by density gradient centrifugation; their specific surface markers were examined as was their ability to differentiate. MSCs were then transfected with pcDNA 3.1-hVEGF 165 or control-containing liposomes. Rats in the experimental MI groups received transfected MSCs, MSCs alone, or gene-transfection alone; controls included a no intervention MI group and a group that was not subjected to ischemia. Among the results, MSCs were successfully isolated and cultured. Among the intervention groups, those that received transplantation of MSCs expressing hVEGF 165 included the smallest areas of infarction and demonstrated the best recovery of cardiac function overall. Moreover, capillary density detected in this group was significantly greater than in the control group and likewise greater than in rats transplanted with MSCs alone. BrdU and Troponin-T staining revealed differential increases in the number of viable cardiomyocytes within the infarction areas; some cardiomyocytes were double-positive. Likewise, evaluation using RT-PCR revealed higher expression levels of hVEGF in rats transplanted with transfected cells compared to those treated with gene transfection alone.

Bone marrow mesenchymal stem cells overexpressing GATA-4 improve cardiac function following myocardial infarction

Perfusion ◽  
2019 ◽  
Vol 34 (8) ◽  
pp. 696-704 ◽  
Author(s):  
Ji-Gang He ◽  
Hong-Rong Li ◽  
Bei-Bei Li ◽  
Qiao-Li Xie ◽  
Dan Yan ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cardiac Function ◽  
Cardiac Tissue ◽  
Cell Group ◽  
Marrow Mesenchymal Stem Cells

Introduction: The present study aimed to examine whether GATA-4 overexpressing bone marrow mesenchymal stem cells can improve cardiac function in a murine myocardial infarction model compared with bone marrow mesenchymal stem cells alone. Methods: A lentiviral-based transgenic system was used to generate bone mesenchymal stem cells which stably expressed GATA-4 (GATA-4-bone marrow mesenchymal stem cells). Apoptosis and the myogenic phenotype of the bone marrow mesenchymal stem cells were measured using Western blot and immunofluorescence assays co-cultured with cardiomyocytes. Cardiac function, bone marrow mesenchymal stem cell homing, cardiac cell apoptosis, and vessel number following transplantation were assessed, as well as the expression of c-Kit. Results: In GATA-4-bone marrow mesenchymal stem cells-cardiomyocyte co-cultures, expression of myocardial-specific antigens, cTnT, connexin-43, desmin, and α-actin was increased compared with bone marrow mesenchymal stem cells alone. Caspase 8 and cytochrome C expression was lower, and the apoptotic rate was significantly lower in GATA-4 bone marrow mesenchymal stem cells. Cardiac function following myocardial infarction was also increased in the GATA-4 bone marrow mesenchymal stem cell group as demonstrated by enhanced ejection fraction and left ventricular fractional shortening. Analysis of the cardiac tissue revealed that the GATA-4 bone marrow mesenchymal stem cell group had a greater number of DiR-positive cells suggestive of increased homing and/or survival. Transplantation with GATA-4-bone marrow mesenchymal stem cells significantly increased the number of blood vessels, decreased the proportion of apoptotic cells, and increased the mean number of cardiac c-kit-positive cells. Conclusion: GATA-4 overexpression in bone marrow mesenchymal stem cells exerts anti-apoptotic effects by targeting cytochrome C and Fas pathways, promotes the aggregation of bone marrow mesenchymal stem cells in cardiac tissue, facilitates angiogenesis, and effectively mobilizes c-kit-positive cells following myocardial infarction, leading to the improvement of cardiac function after MI.

Effect of Bone Marrow Mesenchymal Stem Cells (BMSCs) on Cardiac Function and Ventricular Remodeling in Diabetic Rat Models Through Inhibiting MicroRNA (miR)-34a

2021 ◽  
Vol 11 (8) ◽  
pp. 1490-1496
Author(s):  
Xiangzhen Qu ◽  
Shanyong Zhu
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cardiac Function ◽  
Ventricular Remodeling ◽  
Heart Function ◽  
Negative Relationship ◽  
Left Ventricular ◽  
Ventricular Wall ◽  
Marrow Mesenchymal Stem Cells

Diabetes mellitus (DM) is harmful to heart tissue. We transplanted bone marrow mesenchymal stem cells (BMSCs) in this study to improve the function of diabetic heart. 45 rats were divided into healthy group, DM group (treated with high-fat diet), and BMSCs group (BMSCs transplantation into the ventricular wall) followed by analysis of miR-34a and aldehyde dehydrogenase 2 (ALDH2) levels, heart function, and ventricular remodeling in the three groups of rats. BMSCs were successfully cultured with high purity, and the myocardium in the BMSCs group was successfully labeled by DAPI. The left ventricular hypertrophy index and myocardial tissue CVF of rats with DM increased significantly, suggesting that ventricular remodeling in DM rats was stimulated. However, when BMSCs were transplanted to the ventricular wall, although the ventricular remodeling was not completely avoided, apoptosis of BMSCs group was significantly reduced. MiR-34a relative level in BMSCs group was obviously elevated, whereas ALDH2 level showed opposite trend with a negative relationship. MiR-34a can effectively bind to the 3′-UTR of ALDH2 mRNA, indicating that it can directly regulate gene expression. BMSCs can promote the synthesis of ALDH2 by inhibiting the expression of miR-34a, and improve the cardiac function and ventricular remodeling of diabetic rats.

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