scholarly journals Immunohistochemical expression levels of p53 and eIF4E markers in histologically negative surgical margins, and their association with the clinical outcome of patients with head and neck squamous cell carcinoma

2015 ◽  
Vol 4 (2) ◽  
pp. 166-172 ◽  
Author(s):  
JAGTAR SINGH ◽  
RAMA JAYARAJ ◽  
SIDDHARTHA BAXI ◽  
MARIANA MILEVA ◽  
JOHN SKINNER ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Clinical Outcome ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Surgical Margins ◽  
Neck Squamous Cell Carcinoma ◽  
Immunohistochemical Expression ◽  
Expression Levels

Expression levels of miR-34-family microRNAs are associated with TP53 mutation status in head and neck squamous cell carcinoma

2018 ◽  
Vol 276 (2) ◽  
pp. 521-533 ◽  
Author(s):  
Chanatip Metheetrairut ◽  
Chanticha Chotigavanich ◽  
Kanchana Amornpichetkul ◽  
Phawin Keskool ◽  
Sunun Ongard ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Tp53 Mutation ◽  
Neck Squamous Cell Carcinoma ◽  
Mutation Status ◽  
Expression Levels

691: SERPINE1 immunostaining is associated with clinical outcome in head and neck squamous cell carcinoma

2014 ◽  
Vol 50 ◽  
pp. S166
Author(s):  
M. Téllez-Gabriel ◽  
A. Gallardo ◽  
I. Arroyo-Solera ◽  
L.C. Navas ◽  
X. León ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Clinical Outcome ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma

scholarly journals Purine Metabolites in Tumor-Derived Exosomes May Facilitate Immune Escape of Head and Neck Squamous Cell Carcinoma

Cancers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1602 ◽  
Author(s):  
Nils Ludwig ◽  
Delbert G. Gillespie ◽  
Torsten E. Reichert ◽  
Edwin K. Jackson ◽  
Theresa L. Whiteside
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Immune Escape ◽  
Neck Squamous Cell Carcinoma ◽  
Early Stage Disease ◽  
Expression Levels ◽  
Purine Metabolites ◽  
Purine Metabolite

Body fluids of patients with head and neck squamous cell carcinoma (HNSCC) are enriched in exosomes that reflect properties of the tumor. The aim of this study was to determine whether purine metabolites are carried by exosomes and evaluate their role as potential contributors to tumor immune escape. The gene expression levels of the purine synthesis pathway were studied using the Cancer Genome Atlas (TCGA) Head and Neck Cancer database. Exosomes were isolated from supernatants of UMSCC47 cells and from the plasma of HNSCC patients (n = 26) or normal donors (NDs; n = 5) using size exclusion chromatography. Ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) was used to assess levels of 19 purine metabolites carried by exosomes. In HNSCC tissues, expression levels of genes involved in the purinergic pathway were upregulated indicating an accelerated purine metabolism compared to normal tissues. Exosomes from supernatants of UMSCC47 cells contained several purine metabolites, predominantly adenosine and inosine. Purine metabolite levels were enriched in exosomes isolated from the plasma of HNSCC patients compared to those isolated from NDs and carried elevated levels of adenosine (p = 0.0223). Exosomes of patients with early-stage disease and no lymph node metastasis contained significantly elevated levels of adenosine and 5′-GMP (p = 0.0247 and p = 0.0229, respectively). The purine metabolite levels in exosomes decreased in patients with advanced cancer and nodal involvement. This report provides the first evidence that HNSCC cells shuttle purine metabolites in exosomes, with immunosuppressive adenosine being the most prominent purine. Changes in the content and levels of purine metabolites in circulating exosomes reflect disease progression in HNSCC patients.

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