INHIBITION OF B16 MELANOMA METASTASIS BY ADMINISTRATION OF G(M3)- OR GG3- LIPOSOMES - BLOCKING ADHESION OF MELANOMA-CELLS TO ENDOTHELIAL-CELLS (ANTIADHESION THERAPY) VIA INHIBITION OF G(M3)-GG3CER OR G(M3)-LACCER INTERACTION

1995 ◽  
Author(s):  
E OTSUJI ◽  
YS PARK ◽  
K TASHIRO ◽  
N KOJIMA ◽  
T TOYOKUNI ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Melanoma Metastasis

scholarly journals Ligand-directed targeting of lymphatic vessels uncovers mechanistic insights in melanoma metastasis

2015 ◽  
Vol 112 (8) ◽  
pp. 2521-2526 ◽  
Author(s):  
Dawn R. Christianson ◽  
Andrey S. Dobroff ◽  
Bettina Proneth ◽  
Amado J. Zurita ◽  
Ahmad Salameh ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Lymphatic Vessels ◽  
Melanoma Cells ◽  
Cell Interactions ◽  
Melanoma Metastasis ◽  
Lymphatic Endothelium ◽  
Lymphatic Endothelial Cells ◽  
Cell Surfaces ◽  
Surface Binding ◽  
Lymphatic Vasculature

Metastasis is the most lethal step of cancer progression in patients with invasive melanoma. In most human cancers, including melanoma, tumor dissemination through the lymphatic vasculature provides a major route for tumor metastasis. Unfortunately, molecular mechanisms that facilitate interactions between melanoma cells and lymphatic vessels are unknown. Here, we developed an unbiased approach based on molecular mimicry to identify specific receptors that mediate lymphatic endothelial–melanoma cell interactions and metastasis. By screening combinatorial peptide libraries directly on afferent lymphatic vessels resected from melanoma patients during sentinel lymphatic mapping and lymph node biopsies, we identified a significant cohort of melanoma and lymphatic surface binding peptide sequences. The screening approach was designed so that lymphatic endothelium binding peptides mimic cell surface proteins on tumor cells. Therefore, relevant metastasis and lymphatic markers were biochemically identified, and a comprehensive molecular profile of the lymphatic endothelium during melanoma metastasis was generated. Our results identified expression of the phosphatase 2 regulatory subunit A, α-isoform (PPP2R1A) on the cell surfaces of both melanoma cells and lymphatic endothelial cells. Validation experiments showed that PPP2R1A is expressed on the cell surfaces of both melanoma and lymphatic endothelial cells in vitro as well as independent melanoma patient samples. More importantly, PPP2R1A-PPP2R1A homodimers occur at the cellular level to mediate cell–cell interactions at the lymphatic–tumor interface. Our results revealed that PPP2R1A is a new biomarker for melanoma metastasis and show, for the first time to our knowledge, an active interaction between the lymphatic vasculature and melanoma cells during tumor progression.

scholarly journals Elucidation of Melanogenesis-Associated Signaling Pathways Regulated by Argan Press Cake in B16 Melanoma Cells

Nutrients ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 2697
Author(s):  
Thouria Bourhim ◽  
Myra O. Villareal ◽  
Chemseddoha Gadhi ◽  
Hiroko Isoda
Keyword(s):  
Signaling Pathways ◽  
Press Cake ◽  
Global Gene Expression ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Dependent Manner ◽  
Melanin Biosynthesis ◽  
B16 Melanoma Cells ◽  
Pigmentary Disorders ◽  
Argan Oil

The beneficial effect on health of argan oil is recognized worldwide. We have previously reported that the cake that remains after argan oil extraction (argan press-cake or APC) inhibits melanogenesis in B16 melanoma cells in a time-dependent manner without cytotoxicity. In this study, the global gene expression profile of B16 melanoma cells treated with APC extract was determined in order to gain an understanding of the possible mechanisms of action of APC. The results suggest that APC extract inhibits melanin biosynthesis by down-regulating microphthalmia-associated transcription factor (Mitf) and its downstream signaling pathway through JNK signaling activation, and the inhibition of Wnt/β-catenin and cAMP/PKA signaling pathways. APC extract also prevented the transport of melanosomes by down-regulating Rab27a expression. These results suggest that APC may be an important natural skin whitening product and pharmacological agent used for clinical treatment of pigmentary disorders.

scholarly journals Effect of Phlorotannins Isolated From Eisenia bicyclis on Melanogenesis in Mouse B16 Melanoma Cells

2021 ◽  
Vol 16 (5) ◽  
pp. 1934578X2110192
Author(s):  
Yuki Ohno ◽  
Shiori Kondo ◽  
Kiho Tajima ◽  
Toshiyuki Shibata ◽  
Tomohiro Itoh
Keyword(s):  
Melanoma Cells ◽  
B16 Melanoma ◽  
Physiological Effects ◽  
Related Protein ◽  
Eisenia Bicyclis ◽  
Melanin Production ◽  
Melanocyte Stimulating Hormone ◽  
B16 Melanoma Cells ◽  
Anti Cancer ◽  
Tyrosinase Related Protein

Phlorotannins isolated from brown algae, such as Eisena bicyclis, have positive physiological effects, including anti-cancer, anti-inflammatory, and anti-Alzheimer’s disease. Although phlorotannins have been shown to inhibit tyrosinase, an enzyme essential for melanogenesis, their effect on melanogenesis remains unexplored. Thus, we isolated phlorotannins from E. bicyclis and examined their effects on α-melanocyte-stimulating hormone (α-MSH)-induced melanogenesis in murine B16 melanoma cells. Both fucofuroeckol-A (FF-A) and phlorofucofuroeckol-A (PFF-A) suppressed α-MSH-induced melanogenesis. Neither inhibited human tyrosinase (TYR) activity, but both inhibited tyrosinase-related protein-2 activity. FF-A downregulated the expression of microphthalmia-associated transcription factor and TYR, which subsequently suppressed melanin production. These results suggest that phlorotannins could be beneficial as melanin control drugs for hyperpigmentation disorders.

Saponified Evening Primrose Oil Reduces Melanogenesis in B16 Melanoma Cells and Reduces UV-Induced Skin Pigmentation in Humans

Lipids ◽  
2010 ◽  
Vol 45 (5) ◽  
pp. 401-407 ◽  
Author(s):  
Jeung-Hyun Koo ◽  
Ikjae Lee ◽  
Seok-Kweon Yun ◽  
Han-Uk Kim ◽  
Byung-Hyun Park ◽  
...  
Keyword(s):  
Skin Pigmentation ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Evening Primrose Oil ◽  
Evening Primrose ◽  
B16 Melanoma Cells

Effect of restraint stress on immune system and experimental B16 melanoma metastasis in aged mice

1997 ◽  
Vol 93 (1-3) ◽  
pp. 107-117 ◽  
Author(s):  
Jun Kanno ◽  
Atsuko Wakikawa ◽  
Masanori Utsuyama ◽  
Katsuiku Hirokawa
Keyword(s):  
Immune System ◽  
Restraint Stress ◽  
B16 Melanoma ◽  
Melanoma Metastasis ◽  
Aged Mice

Combined Treatment of Adriamycin and Dipyridamole Inhibits Lung Metastasis of B16 Melanoma Cells in Mice

1990 ◽  
Vol 22 (4) ◽  
pp. 213-218 ◽  
Author(s):  
Y. Sakaguchi ◽  
Y. Emi ◽  
Y. Maehara ◽  
S. Kohnoe ◽  
K. Sugimachi
Keyword(s):  
Lung Metastasis ◽  
Combined Treatment ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
B16 Melanoma Cells
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