ANTITUMOR-PROMOTING ACTIVITY OF OLIGOMERIC PROANTHOCYANIDINS IN MOUSE EPIDERMIS IN-VIVO

1994 ◽  
Author(s):  
XM GAO ◽  
EM PERCHELLET ◽  
HU GALI ◽  
L RODRIGUEZ ◽  
RW HEMINGWAY ◽  
...  
Keyword(s):  
Mouse Epidermis ◽  
Oligomeric Proanthocyanidins

scholarly journals Percutaneous Penetration of Methylglyoxal Bis(guanylhydrazone): Effects on Hairless Mouse Epidermis In Vivo

1983 ◽  
Vol 81 (5) ◽  
pp. 388-392 ◽  
Author(s):  
Jerry L. McCullough ◽  
Gerald D. Weinstein ◽  
Michael G. Rosenblum ◽  
Jennifer J. Jenkins
Keyword(s):  
Hairless Mouse ◽  
Percutaneous Penetration ◽  
Mouse Epidermis

scholarly journals Identification of two intermediates during processing of profilaggrin to filaggrin in neonatal mouse epidermis.

1984 ◽  
Vol 99 (4) ◽  
pp. 1372-1378 ◽  
Author(s):  
K A Resing ◽  
K A Walsh ◽  
B A Dale
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Tandem Repeats ◽  
Peptide Mapping ◽  
Two Dimensional ◽  
Major Event ◽  
Mouse Epidermis ◽  
Cell Stage ◽  
Processing Steps

A major event in the keratinization of epidermis is the production of the histidine-rich protein filaggrin (26,000 mol wt) from its high molecular weight (greater than 350,000) phosphorylated precursor (profilaggrin). We have identified two nonphosphorylated intermediates (60,000 and 90,000 mol wt) in NaSCN extracts of epidermis from C57/Bl6 mice by in vivo pulse-chase studies. Results of peptide mapping using a two-dimensional technique suggest that these intermediates consist of either two or three copies of filaggrin domains. Each of the intermediates has been purified. The ratios of amino acids in the purified components are unusual and essentially identical. The data are discussed in terms of a precursor containing tandem repeats of similar domains. In vivo pulse-chase experiments demonstrate that the processing of the high molecular weight phosphorylated precursor involves dephosphorylation and proteolytic steps through three-domain and two-domain intermediates to filaggrin. These processing steps appear to occur as the cell goes through the transition cell stage to form a cornified cell.

scholarly journals Ultraviolet B radiation induction of ornithine decarboxylase gene expression in mouse epidermis

1990 ◽  
Vol 270 (3) ◽  
pp. 565-568 ◽  
Author(s):  
C F Rosen ◽  
D Gajic ◽  
Q Jia ◽  
D J Drucker
Keyword(s):  
Gene Expression ◽  
Ornithine Decarboxylase ◽  
Hairless Mouse ◽  
Ultraviolet B ◽  
Dependent Manner ◽  
Mouse Epidermis ◽  
Cellular Effects ◽  
Mrna Transcripts ◽  
Maximal Induction

The cellular effects of u.v. radiation have been studied by using a hairless-mouse model in vivo. U.v. B radiation (u.v.B) induced the activity of the enzyme ornithine decarboxylase (ODC) in mouse epidermis. Maximal induction was noted after radiation with 90 mJ/cm2, and increased ODC activity was first detected 2 h after u.v.B exposure. U.v.B. also induced the expression of the ODC gene in a time- and dose-dependent manner, but did not induce the levels of actin mRNA transcripts. Cycloheximide treatment did not alter basal levels of ODC mRNA transcripts and had no effect on the u.v.B induction of ODC-gene expression. The results of these experiments demonstrate that u.v.B radiation induces both the expression of the ODC gene and the activity of the enzyme, and provides a useful ‘in vivo’ paradigm for the analysis of the molecular effects of u.v.B radiation.

Formation and persistence of benzo[a]pyrene-DNA adducts in mouse epidermis in vivo: importance of adduct conformation

1995 ◽  
Vol 16 (10) ◽  
pp. 2561-2569 ◽  
Author(s):  
M. Suh ◽  
F. Ariese ◽  
G.J. Small ◽  
R. Jankowiak ◽  
A. Hewer ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Mouse Epidermis

scholarly journals Grape Seeds Proanthocyanidins: An Overview of In Vivo Bioactivity in Animal Models

Nutrients ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 2435 ◽  
Author(s):  
Rodríguez-Pérez ◽  
García-Villanova ◽  
Guerra-Hernández ◽  
Verardo
Keyword(s):  
Oxidative Stress ◽  
Animal Studies ◽  
Grape Seeds ◽  
Oligomeric Proanthocyanidins ◽  
Well Design ◽  
Public Health Organizations ◽  
The Impact ◽  
By Products ◽  
Shed Light

Over the last decade, proanthocyanidins (PACs) are attracting attention not only from the food industry but also from public health organizations due to their health benefits. It is well-known that grapes are a good source of PACs and for that reason, the industry is also focused on grape by-products identification and bioactivity evaluation. Grape seeds extract (GSPE) is a rich source of PACs, mainly composed of monomeric catechin and epicatechin, gallic acid and polymeric and oligomeric proanthocyanidins. Thus, this review encompasses the state-of-art structure and the most recent evidence about the impact of GSPE on chronic diseases, with a focus on oxidative stress, inflammation and metabolic syndrome (MeS)-related disorders such as obesity, diabetes and cardiovascular risk disease in vivo to offer new perspectives in the field that allow further research. Despite the controversial results, is undeniable that PACs from grape seeds are highly antioxidants, thus, the capacity of GSPE to improve oxidative stress might mediate the inflammation process and the progress of MeS-related pathologies. However, further well-design animal studies with standardized dosages and GSPE composition are necessary to shed light into the cause-effect relationship in a more accurate way to later allow a deeper study of the effect of GSPE in humans.

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