Targeted inhibition of NMYC by peptide nucleic acid in N-myc amplified human neuroblastoma cells: cell-cycle inhibition with induction of neuronal cell differentiation and apoptosis

2004 ◽  
Author(s):  
Andrea Pession ◽  
Roberto Tonelli ◽  
Raffaele Fronza ◽  
Elena Sciamanna ◽  
Roberto Corradini ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Neuroblastoma Cells ◽  
Neuronal Cell ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Neuronal Cell Differentiation ◽  
Cell Cycle Inhibition ◽  
Targeted Inhibition

scholarly journals Zingerone Suppresses Tumor Development through Decreasing Cyclin D1 Expression and Inducing Mitotic Arrest

2018 ◽  
Vol 19 (9) ◽  
pp. 2832 ◽  
Author(s):  
Jae-Sun Choi ◽  
Jaewook Ryu ◽  
Woom-Yee Bae ◽  
Aron Park ◽  
Seungyoon Nam ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cyclin D1 ◽  
Molecular Mechanisms ◽  
Neuroblastoma Cells ◽  
Mitotic Arrest ◽  
Therapeutic Drug ◽  
Mouse Tumor ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Mitotic Cells

Cancer cells undergo uncontrolled proliferation resulting from aberrant activity of various cell-cycle proteins. Therefore, despite recent advances in intensive chemotherapy, it is difficult to cure cancer completely. Recently, cell-cycle regulators became attractive targets in cancer therapy. Zingerone, a phenolic compound isolated from ginger, is a nontoxic and inexpensive compound with varied pharmacological activities. In this study, the therapeutic effect of zingerone as an anti-mitotic agent in human neuroblastoma cells was investigated. Following treatment of BE(2)-M17 cells with zingerone, we performed a 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay and colony-formation assay to evaluate cellular proliferation, in addition to immunofluorescence cytochemistry and flow cytometry to examine the mitotic cells. The association of gene expression with tumor stage and survival was analyzed. Furthermore, to examine the anti-cancer effect of zingerone, we applied a BALB/c mouse-tumor model using a BALB/c-derived adenocarcinoma cell line. In human neuroblastoma cells, zingerone inhibited cellular viability and survival. Moreover, the number of mitotic cells, particularly those in prometaphase, increased in zingerone-treated neuroblastoma cells. Regarding specific molecular mechanisms, zingerone decreased cyclin D1 expression and induced the cleavage of caspase-3 and poly (ADP-ribose) polymerase 1 (PARP-1). The decrease in cyclin D1 and increase in histone H3 phosphorylated (p)-Ser10 were confirmed by immunohistochemistry in tumor tissues administered with zingerone. These results suggest that zingerone induces mitotic arrest followed by inhibition of growth of neuroblastoma cells. Collectively, zingerone may be a potential therapeutic drug for human cancers, including neuroblastoma.

scholarly journals The transcription of the human fructose-bisphosphate aldolase C gene is activated by nerve-growth-factor-induced B factor in human neuroblastoma cells*

1997 ◽  
Vol 323 (1) ◽  
pp. 245-250 ◽  
Author(s):  
Pasqualina BUONO ◽  
Lisa de CONCILIIS ◽  
Paola IZZO ◽  
Francesco SALVATORE
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Neuroblastoma Cells ◽  
Neuronal Cell ◽  
Fructose Bisphosphate ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Nerve Growth ◽  
Aldolase C ◽  
Fructose Bisphosphate Aldolase

A DNA region located at around -200 bp in the 5´ flanking region (region D) of the human brain-type fructose-bisphosphate aldolase (aldolase C) gene has been analysed. We show by transient transfection assay and electrophoretic-mobility-shift assay (EMSA) that the binding of transcriptional activators to region D is much more efficient (80% versus 30%) in human neuroblastoma cells (SKNBE) than in the non-neuronal cell line A1251, which contains low levels of aldolase C mRNA. The sequence of region D, CAAGGTCA, is very similar to the AAAGGTCA motif present in the mouse steroid 21-hydroxylase gene; the latter motif binds nerve-growth-factor-induced B factor (NGFI-B), which is a member of the thyroid/steroid/retinoid nuclear receptor gene family. Competition experiments in EMSA and antibody-directed supershift experiments showed that NGFI-B is involved in the binding to region D of the human aldolase C gene. Furthermore, the regulation of the aldolase C gene (which is the second known target of NGFI-B) expression during development parallels that of NGFI-B.

scholarly journals Key role for p27Kip1, retinoblastoma protein Rb, and MYCN in polyamine inhibitor-induced G1 cell cycle arrest in MYCN-amplified human neuroblastoma cells

Oncogene ◽  
2005 ◽  
Vol 24 (36) ◽  
pp. 5606-5618 ◽  
Author(s):  
Christopher J Wallick ◽  
Ivonne Gamper ◽  
Mike Thorne ◽  
David J Feith ◽  
Kelsie Y Takasaki ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Neuroblastoma Cells ◽  
Retinoblastoma Protein ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Cycle Arrest ◽  
G1 Cell Cycle Arrest

scholarly journals Involvement of Dihydroceramide Desaturase in Cell Cycle Progression in Human Neuroblastoma Cells

2007 ◽  
Vol 282 (23) ◽  
pp. 16718-16728 ◽  
Author(s):  
Jacqueline M. Kraveka ◽  
Li Li ◽  
Zdzislaw M. Szulc ◽  
Jacek Bielawski ◽  
Besim Ogretmen ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Cycle Progression ◽  
Dihydroceramide Desaturase
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