scholarly journals The nerve growth factor alters calreticulin translocation from the endoplasmic reticulum to the cell surface and its signaling pathway in epithelial ovarian cancer cells

2017 ◽  
Vol 50 (4) ◽  
pp. 1261-1270 ◽  
Author(s):  
Carolina Andrea Vera ◽  
Lorena Oróstica ◽  
Fernando Gabler ◽  
Arturo Ferreira ◽  
Alberto Selman ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Endoplasmic Reticulum ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cell Surface ◽  
Epithelial Ovarian Cancer ◽  
Cancer Cells ◽  
Signaling Pathway ◽  
Ovarian Cancer Cells ◽  
Nerve Growth

scholarly journals Metformin prevents nerve growth factor-dependent proliferative and proangiogenic effects in epithelial ovarian cancer cells and endothelial cells

2018 ◽  
Vol 10 ◽  
pp. 175883591877098 ◽  
Author(s):  
Maritza P. Garrido ◽  
Carolina Vera ◽  
Margarita Vega ◽  
Andrew F.G. Quest ◽  
Carmen Romero
Keyword(s):  
Ovarian Cancer ◽  
Cell Proliferation ◽  
Endothelial Cells ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Epithelial Ovarian Cancer ◽  
Ovarian Cancer Cells ◽  
Nerve Growth ◽  
Angiogenic Potential ◽  
Ea.Hy926 Cells

Background: Epithelial ovarian cancer (EOC) is characterized by exacerbated angiogenesis regulated by proangiogenic and growth factors. Nerve growth factor (NGF) is overexpressed in EOC where it promotes proliferation as well as survival and is considered a proangiogenic factor. Metformin, a drug commonly used in the treatment of diabetes, is attributed to antineoplastic effects, but the underlying mechanisms remain unknown. Given that current therapies yield modest results in EOC patients, the aim of this study was to determine the effects of metformin on NGF-enhanced proliferation of EOC cells and the angiogenic potential of endothelial cells. Methods: A2780 (EOC), HOSE (human ovarian surface epithelial) and EA.hy926 (endothelial) cells were treated with NGF and metformin. Cell viability, cell proliferation and cell cycle were evaluated in all three cell lines, and the angiogenic potential in endothelial EA.hy926 cells. Results: NGF enhanced cell proliferation in A2780, HOSE and EA.hy926 cells ( p < 0.05), while metformin treatment decreased cell proliferation in A2780 and EA.hy926 cells ( p < 0.05). Moreover, the NGF-enhanced angiogenic score in EA.hy926 cells was prevented by metformin ( p < 0.05). Conclusions: Given that NGF plays a significant role in EOC progression, our current findings suggest that metformin holds considerable promise as an adjuvant treatment in ovarian cancer.

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