scholarly journals Prolonged survival in pancreatic cancer patients with increased regucalcin gene expression: Overexpression of regucalcin suppresses the proliferation in human pancreatic cancer MIA PaCa-2 cells in vitro

2016 ◽  
Vol 48 (5) ◽  
pp. 1955-1964 ◽  
Author(s):  
MASAYOSHI YAMAGUCHI ◽  
SATORU OSUKA ◽  
M. NEALE WEITZMANN ◽  
BASSEL F. EL-RAYES ◽  
MAMORU SHOJI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pancreatic Cancer ◽  
Cancer Patients ◽  
Prolonged Survival ◽  
Human Pancreatic Cancer ◽  
Regucalcin Gene

scholarly journals Prolonged survival in hepatocarcinoma patients with increased regucalcin gene expression: HepG2 cell proliferation is suppressed by overexpression of regucalcin in vitro

2016 ◽  
Vol 49 (4) ◽  
pp. 1686-1694 ◽  
Author(s):  
Masayoshi Yamaguchi ◽  
Satoru Osuka ◽  
M. Neale Weitzmann ◽  
Bassel F. El-Rayes ◽  
Mamoru Shoji ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Hepg2 Cell ◽  
Prolonged Survival ◽  
Regucalcin Gene

scholarly journals In VitroGlobal Gene Expression Analyses Support the Ethnopharmacological Use ofAchyranthes aspera

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Pochi R. Subbarayan ◽  
Malancha Sarkar ◽  
Lubov Nathanson ◽  
Nikesh Doshi ◽  
Balakrishna L. Lokeshwar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pancreatic Cancer ◽  
Mode Of Action ◽  
Gene Expression Regulation ◽  
Pancreatic Cancer Cell Line ◽  
Human Pancreatic Cancer ◽  
Anticancer Properties ◽  
Thomson Reuters ◽  
Gene Expression Analyses

Achyranthes aspera(familyAmaranthaceae) is known for its anticancer properties. We have systematically validated thein vitroandin vivoanticancer properties of this plant. However, we do not know its mode of action. Global gene expression analyses may help decipher its mode of action. In the absence of identified active molecules, we believe this is the best approach to discover the mode of action of natural products with known medicinal properties. We exposed human pancreatic cancer cell line MiaPaCa-2 (CRL-1420) to 34 μg/mL of LE for 24, 48, and 72 hours. Gene expression analyses were performed using whole human genome microarrays (Agilent Technologies, USA). In our analyses, 82 (54/28) genes passed the quality control parameter, set at FDR ≤ 0.01 and FC of ≥±2. LE predominantly affected pathways of immune response, metabolism, development, gene expression regulation, cell adhesion, cystic fibrosis transmembrane conductance regulation (CFTR), and chemotaxis (MetaCore tool (Thomson Reuters, NY)). Disease biomarker enrichment analysis identified LE regulated genes involved in Vasculitis—inflammation of blood vessels. Arthritis and pancreatitis are two of many etiologies for vasculitis. The outcome of disease network analysis supports the medicinal use ofA. aspera, viz, to stop bleeding, as a cure for pancreatic cancer, as an antiarthritic medication, and so forth.

Serum Neurotensin in Human Pancreatic Cancer

1996 ◽  
Vol 82 (6) ◽  
pp. 592-595 ◽  
Author(s):  
Tamara Meggiato ◽  
Chiara Ferrara ◽  
Giovanna Tessari ◽  
Mario Plebani ◽  
Massimo De Paoli ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Chronic Pancreatitis ◽  
Cancer Patients ◽  
Human Cancer ◽  
Stage Iv ◽  
Serum Levels ◽  
Human Pancreatic Cancer ◽  
Human Cancer Cells ◽  
Control Subjects

The role of neurotensin as a physiologic regulator of exocrine pancreatic secretion is known, but the hormone has only recently been recognized as important mitogen in vitro for human cancer cells. The aim of this study was to evaluate the variations of serum levels of neurotensin in pancreatic cancer. We studied 58 patients: 13 control subjects, 20 pancreatic cancer patients, 11 chronic pancreatitis patients, and 14 cases of extrapancreatic disease. No differences were found between serum values of neurotensin in pancreatic cancer and control subjects or extrapancreatic disease. Significantly higher values were detected in chronic pancreatitis than in pancreatic cancer patients ( P <0.04). In chronic pancreatitis patients, the serum levels of neurotensin were correlated with serum amylase ( r = 0.95, P <0.01). Lower levels of neurotensin were found in stage IV pancreatic cancer than in stages I-II ( t = 1.82, P <0.04) and in grade II than in grade I ( t = 2.21, P <0.02). Significant correlations were found between serum levels of neurotensin and two indices of nutrition: albumin ( r = 0.60, P <0.05) and the percentage reduction in body weight (Z = 2.20, P <0.02). No correlations were found between serum levels of the hormone and size of the neoplasm or the survival of patients. We can conclude that the serum variations of neurotensin do not seem to be related to the progression of human pancreatic cancer. The variation of serum levels of the hormone may be linked to a poor nutritional status of the patient.

scholarly journals Expression of Sortilin in Pancreatic Carcinoma and Influence of Pancreatic Cancer Cells by Sortilin siRNA Knockdown

2021 ◽  
Author(s):  
Di Xu ◽  
Fan Yu ◽  
Shangqing Liu ◽  
Cai Rong
Keyword(s):  
Pancreatic Cancer ◽  
Logistic Regression ◽  
Regression Analysis ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Roc Curve ◽  
Univariate Analysis ◽  
Human Pancreatic Cancer ◽  
Pancreatic Cancer Cells

Abstract BackgroundCurrently, there are few effective therapeutic options for pancreatic cancer patients. Sortilin is a member of vps10p receptor family，reported in many types of cancers. However, the underlying mechanism and prognostic value of sortilin in pancreatic cancer are still unclear. ObjectiveUnderstand the expression of sortilin in pancreatic cancer, and analyze its mechanism that affects the occurrence and development of pancreatic cancer. MethodsA tissue microarray of 115 pancreatic cancer metastases was analyzed by immunohistochemistry. All data were analyzed by univariate analysis and multivariate analysis. Multivariate logistic regression analysis and the area under the receiver operating characteristic (ROC) curve were used to analyze the ability of sortilin in predicting pancreatic cancer. Next, survival analysis was performed to compare the survival time between high-risk and low-risk patients to validate the prognosis prediction efficacy of sortilin. The effects of sortilin on the invasion，metastasis and proliferation of pancreatic cancer cells were investigated both in vitro. investigate the anti-cancer effect of soritlin on human pancreatic cancer Capna1 and Bxpc3 cells, and its possible molecular mechanism. ResultsDifferential expression analysis of 115 tissue microarrays showed sortilin expression was up‐regulated in pancreatic cancer tissues,and it mainly comes from the nucleus. Sortilin expression in nucleus(SEIN) was only negatively correlated with N stage, Binary logistic regression model showed that SEIN is a good diagnostic marker for predicting pancreatic cancer and the accuracy of prediction is as high as 79.1 %. ROC curve analysis demonstrated a statistically significant diagnostic value of SEIN,and the diagnostic accuracy was 86.3%, The Youden Index was calculated to evaluate the diagnostic power, The cut-off value for SEIN in pancreatic cancer diagnosis was 0.85, with a sensitivity of 90.9% and a specificity of 68.3%. Univariate analysis showed that M stage(P=0.022), histological grade(P=0.021), clinic stage(P=0.030)and SEIN(P=0.039) were correlated with prognosis of pancreatic cancer patients, Multivariate regression analysis showed that M stage(P=0.036)and ESIN(P=0.004) were independent factors.The proliferation, invasion and migration of pancreatic cancer cells were inhibited in vitro by sortilin siRNA knockdown.it may have something to do with soritlin/P53/NFκB regulated the the proliferation function and sortilin/MMP9 regulated the invasion-promotion of pancreatic cancer cells. ConclusionsThese fndings demonstrated that the low expression of SEIN indicates better prognosis in pancreatic cancer and supplemented the effect mechanism of sortilin on pancreatic cancer cells. SEIN expression may serve as a potential diagnostic indicator of pancreatic cancer.

In Vitro and In Vivo Antitumor Efficacy of Docetaxel and Sorafenib Combination in Human Pancreatic Cancer Cells

2010 ◽  
Vol 999 (999) ◽  
pp. 1-11
Author(s):  
P. Ulivi ◽  
C. Arienti ◽  
W. Zoli ◽  
M. Scarsella ◽  
S. Carloni ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Antitumor Efficacy ◽  
Human Pancreatic Cancer ◽  
Pancreatic Cancer Cells

scholarly journals Oncogene APOL1 promotes proliferation and inhibits apoptosis via activating NOTCH1 signaling pathway in pancreatic cancer

2021 ◽  
Vol 12 (8) ◽  
Author(s):  
Jiewei Lin ◽  
Zhiwei Xu ◽  
Junjie Xie ◽  
Xiaxing Deng ◽  
Lingxi Jiang ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Signaling Pathway ◽  
Density Lipoprotein ◽  
Human Pancreatic Cancer ◽  
Luciferase Reporter ◽  
Notch1 Signaling ◽  
In Vivo Experiments ◽  
Notch1 Signaling Pathway

AbstractAPOL1 encodes a secreted high-density lipoprotein, which has been considered as an aberrantly expressed gene in multiple cancers. Nevertheless, the role of APOL1 in the regulatory mechanisms of pancreatic cancer remains unknown and should be explored. We identified APOL1 was abnormally elevated in human pancreatic cancer tissues compared with that in adjacent tissues and was associated with poor prognosis. The effects of APOL1 in PC cell proliferation, cell cycle, and apoptosis was verified via functional in vitro and in vivo experiments. The results showed that knockdown of APOL1 significantly inhibited the proliferation and promoted apoptosis of pancreatic cancer. In addition, we identified APOL1 could be a regulator of NOTCH1 signaling pathway using bioinformatics tools, qRT-PCR, dual-luciferase reporter assay, and western blotting. In summary, APOL1 could function as an oncogene to promote proliferation and inhibit apoptosis through activating NOTCH1 signaling pathway expression in pancreatic cancer; therefore, it may act as a novel therapeutic target for pancreatic cancer.

Sign in / Sign up

Export Citation Format

Share Document