scholarly journals Targeting the effector domain of the myristoylated alanine rich C-kinase substrate enhances lung cancer radiation sensitivity

2014 ◽  
Vol 46 (3) ◽  
pp. 1079-1088 ◽  
Author(s):  
TIMOTHY D. ROHRBACH ◽  
JOHN S. JARBOE ◽  
JOSHUA C. ANDERSON ◽  
HOA Q. TRUMMELL ◽  
PATRICIA H. HICKS ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Radiation Sensitivity ◽  
Effector Domain ◽  
Kinase Substrate

scholarly journals MARCKS phosphorylation is modulated by a peptide mimetic of MARCKS effector domain leading to increased radiation sensitivity in lung cancer cell lines

2016 ◽  
Vol 13 (3) ◽  
pp. 1216-1222 ◽  
Author(s):  
Timothy D. Rohrbach ◽  
Robert B. Jones ◽  
Patricia H. Hicks ◽  
Alice N. Weaver ◽  
Tiffiny S. Cooper ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Radiation Sensitivity ◽  
Cancer Cell Lines ◽  
Lung Cancer Cell ◽  
Effector Domain ◽  
Peptide Mimetic

scholarly journals The Effector Domain of Myristoylated Alanine-rich C Kinase Substrate Binds Strongly to Phosphatidylinositol 4,5-Bisphosphate

2000 ◽  
Vol 276 (7) ◽  
pp. 5012-5019 ◽  
Author(s):  
Jiyao Wang ◽  
Anna Arbuzova ◽  
Gyöngyi Hangyás-Mihályné ◽  
Stuart McLaughlin
Keyword(s):  
Effector Domain ◽  
Kinase Substrate

scholarly journals Downregulation of MicroRNA-330 Correlates with the Radiation Sensitivity and Prognosis of Patients with Brain Metastasis from Lung Cancer

2017 ◽  
Vol 42 (6) ◽  
pp. 2220-2229 ◽  
Author(s):  
Li-Peng Jiang ◽  
Zhi-Tu Zhu ◽  
Yue Zhang ◽  
Chun-Yan He
Keyword(s):  
Lung Cancer ◽  
Logistic Regression ◽  
Radiation Therapy ◽  
Survival Rate ◽  
Brain Metastasis ◽  
Cox Regression ◽  
Radiation Sensitivity ◽  
Extracranial Metastasis ◽  
Radiation Resistant ◽  
N Stage

Background: The present study sought to explore the role of microRNA-330 (miR-330) in predicting the radiation response and prognosis of patients with brain metastasis (BM) from lung cancer (LC). Methods: Patients with BM from LC were identified and classified into radiation-sensitive and radiation-resistant groups according to the overall survival rate, local and distant recurrence rate after conventional whole-brain radiation therapy. Quantitative realtime polymerase chain reaction (qRT-PCR) was used to detect miR-330 expression in serum. Receiver operating characteristic (ROC) curves were used to evaluate the prognostic value of miR-330 for the radiation sensitivity of brain metastasis from LC. Related clinical factors for radiation sensitivity were assessed by logistic regression analysis, and a survival analysis was conducted using COX regression and the Kaplan-Meier method. Results: MiR-330 exhibited lower expression in the radiation-sensitive group than in the radiation-resistant group. The area under the ROC curve of miR-330 for predicting radiation sensitivity was 0.898 (optimal cut-off value, 0.815), with a sensitivity of 71.7% and a specificity of 90.1%. After radiation therapy, patients with low miR-330 expression, compared to patients with high miR-330 expression, displayed a lower survival rate and a median survival time. MiR-330 expression was correlated with extracranial metastasis, maximum BM diameter, tumor-node-metastasis (TNM) stage and node (N) stage. Logistic regression and COX regression analyses revealed that extracranial metastasis, TNM stage, N stage and miR-330 expression were factors that influenced both radiation sensitivity and individual prognostic factors in patients with BM from LC. Conclusions: These findings indicate that the downregulation of miR-330 correlates with radiation sensitivity and poor prognosis in patients with BM from LC.

scholarly journals Inactivation of ribosomal protein S27-like impairs DNA interstrand cross-link repair by destabilization of FANCD2 and FANCI

2020 ◽  
Vol 11 (10) ◽  
Author(s):  
Siyuan Sun ◽  
Hengqian He ◽  
Yuanyuan Ma ◽  
Jie Xu ◽  
Guoan Chen ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Ribosomal Protein ◽  
Cancer Cells ◽  
Fanconi Anemia ◽  
Radiation Sensitivity ◽  
Genome Integrity ◽  
Repair Pathway ◽  
Cross Link ◽  
Accelerated Degradation ◽  
Evolutionarily Conserved

Abstract Ribosomal protein S27-like (RPS27L), an evolutionarily conserved ribosomal protein and a direct p53 target, plays an important role in maintenance of genome integrity. We have previously reported that RPS27L regulates radiation sensitivity via the MDM2-p53 and MDM2-MRN-ATM axes. Whether and how RPS27L modulates DNA interstrand cross-link (ICL) repair is unknown. Here we identified that RPS27L binds to FANCD2 and FANCI, two Fanconi anemia (FA) proteins functioning in ICL repair pathway. Upon RPS27L knockdown, the levels of FANCD2 and FANCI are reduced due to accelerated degradation via p62-mediated autophagy-lysosome pathway, which is abrogated by chloroquine (CQ) treatment or Beclin 1 knockdown. Biologically, RPS27L knockdown suppresses FANCD2 foci formation and impairs ICL repair upon exposure to ICL-inducing agent mitomycin C (MMC) in lung cancer cells. This effect of MMC sensitization can be partially reversed by CQ treatment. Together, our study shows that RPS27L positively regulates ICL repair by binding with FANCD2 and FANCI to prevent their degradation via autophagy-lysosome system.

scholarly journals A peptide that inhibits function of Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) reduces lung cancer metastasis

Oncogene ◽  
2013 ◽  
Vol 33 (28) ◽  
pp. 3696-3706 ◽  
Author(s):  
C-H Chen ◽  
P Thai ◽  
K Yoneda ◽  
K B Adler ◽  
P-C Yang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Metastasis ◽  
Kinase Substrate ◽  
Lung Cancer Metastasis

scholarly journals Location of the Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) Effector Domain in Negatively Charged Phospholipid Bicelles

2003 ◽  
Vol 85 (4) ◽  
pp. 2442-2448 ◽  
Author(s):  
Jeffrey F. Ellena ◽  
M. Christine Burnitz ◽  
David S. Cafiso
Keyword(s):  
Effector Domain ◽  
Kinase Substrate
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