scholarly journals Metformin sensitizes chemotherapy by targeting cancer stem cells and the mTOR pathway in esophageal cancer

2014 ◽  
Vol 45 (2) ◽  
pp. 567-574 ◽  
Author(s):  
SOICHIRO HONJO ◽  
JAFFER A. AJANI ◽  
AILING W. SCOTT ◽  
QIONGRONG CHEN ◽  
HEATH D. SKINNER ◽  
...  
Keyword(s):  
Stem Cells ◽  
Esophageal Cancer ◽  
Cancer Stem Cells ◽  
Mtor Pathway

scholarly journals Genome-Wide DNA Methylation Pattern of Cancer Stem Cells in Esophageal Cancer

2020 ◽  
Vol 19 ◽  
pp. 153303382098379
Author(s):  
Xiying Yu ◽  
Ying Teng ◽  
Xingran Jiang ◽  
Hui Yuan ◽  
Wei Jiang
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Esophageal Cancer ◽  
Cancer Stem Cells ◽  
Side Population ◽  
Methylation Status ◽  
Methylation Profile ◽  
Cpg Dinucleotides ◽  
Genome Wide ◽  
Differentially Methylated Genes

Background: Cancer stem cells (CSCs) are considered the main cause of cancer recurrence and metastasis, and DNA methylation is involved in the maintenance of CSCs. However, the methylation profile of esophageal CSCs remains unknown. Methods: Side population (SP) cells were isolated from esophageal squamous cell carcinoma (ESCC) cell lines KYSE150 and EC109. Sphere-forming cells were collected from human primary esophageal cancer cells. SP cells and sphere-forming cells were used as substitutes for cancer stem-like cells. We investigated the genome-wide DNA methylation profile in esophageal cancer stem-like cells using reduced representation bisulfite sequencing (RRBS). Results: Methylated cytosine (mC) was found mostly in CpG dinucleotides, located mostly in the intronic, intergenic, and exonic regions. Forty intersected differentially methylated regions (DMRs) were identified in these 3 groups of samples. Thirteen differentially methylated genes with the same alteration trend were detected; these included OTX1, SPACA1, CD163L1, ST8SIA2, TECR, CADM3, GRM1, LRRK1, CHSY1, PROKR2, LINC00658, LOC100506688, and NKD2. DMRs covering ST8SIA2 and GRM1 were located in exons. These differentially methylated genes were involved in 10 categories of biological processes and 3 cell signaling pathways. Conclusions: When compared to non-CSCs, cancer stem-like cells have a differential methylation status, which provides an important biological base for understanding esophageal CSCs and developing therapeutic targets for esophageal cancer.

PS02.061: TRANILAST: SPECIFIC INHIBITOR OF TRPV2 IS THERAPEUTIC AGENT OF ESOPHAGEAL CANCER STEM CELLS

2018 ◽  
Vol 31 (Supplement_1) ◽  
pp. 138-138
Author(s):  
Keita Katsurahara ◽  
Atsushi Shiozaki ◽  
Michihiro Kudou ◽  
Katsutoshi Shoda ◽  
Tomohiro Arita ◽  
...  
Keyword(s):  
Stem Cells ◽  
Esophageal Cancer ◽  
Membrane Proteins ◽  
Cancer Stem Cells ◽  
Microarray Analysis ◽  
Therapeutic Agent ◽  
Transient Receptor Potential ◽  
Conflicts Of Interest ◽  
Expression Profiles ◽  
Specific Inhibitor

Abstract Background Recent studies revealed that membrane proteins, such as ion transporters, are specifically activated in cancer stem cells (CSCs). Therefore, these molecules are receiving a great attention as new chemotherapeutic targets of malignant tumor. This study aimed to investigate the expression and activity of ion transport-related molecules in CSCs of esophageal squamous cell carcinoma (ESCC). Methods We sorted cells with high expression of ALDH1A1 via FACS, and then, CSCs were generated using the sphere formation assay. The gene expression profiles of CSCs were examined using a microarray analysis. Candidate genes of membrane proteins activated in CSCs were selected based on that microarray data. Anticancer effects induced by inhibition of the selected proteins were examined. Results ALDH1A1 mRNA and protein levels were certainly upregulated in CSCs compared with non-CSCs. Obtained CSCs were resistant to Cisplatin and had the ability of re-differentiation. The results of the microarray analysis revealed that expressions of 50 genes of plasma membrane proteins were changed in CSCs, and that several genes related to ion channels, including transient receptor potential cation channel subfamily V member 2 (TRPV2), were upregulated. The upregulation of TRPV2 mRNA were also validated in CSCs derived from two types of esophageal cancer cell lines using RT-PCR method. Tranilast, which is specific TRPV2 inhibitor, was more cytotoxic at lower concentration in CSCs than in non-CSCs, and effectively decreased the number of tumorspheres. Further, Tranilast significantly decreased the cell population with high ALDH1A1 expression in esophageal cancer cells. Conclusion The results of the present study suggest that TRPV2 is involved in the maintenance of CSCs, and Tranilast, which is specific inhibitor of TRPV2, becomes a promising targeted therapeutic agent against ESCC. Disclosure All authors have declared no conflicts of interest.

Mo1737 – Aldh2 and Autophaghy-Mediated Redox Homeostasis Limit the Alcohol-Induced Expansion of Esophageal Cancer Stem Cells

2019 ◽  
Vol 156 (6) ◽  
pp. S-827
Author(s):  
Masataka Shimonosono ◽  
Prasanna M. Chandramouleeswaran ◽  
Takeo Hara ◽  
Tatiana Karakasheva ◽  
Takashi Kijima ◽  
...  
Keyword(s):  
Stem Cells ◽  
Esophageal Cancer ◽  
Cancer Stem Cells ◽  
Redox Homeostasis

scholarly journals Esophageal cancer stem cells and implications for future therapeutics

2016 ◽  
pp. 2247 ◽  
Author(s):  
Jing Cai ◽  
Xia Qian ◽  
Cheng Tan ◽  
Feng Wang ◽  
Baixia Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Esophageal Cancer ◽  
Cancer Stem Cells

Effects of Icaritin on the physiological activities of esophageal cancer stem cells

2018 ◽  
Vol 504 (4) ◽  
pp. 792-796 ◽  
Author(s):  
Songchen Han ◽  
Yunjiu Gou ◽  
Dacheng Jin ◽  
Jilong Ma ◽  
Meng Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Esophageal Cancer ◽  
Cancer Stem Cells

Abstract 501: Aldh2 mutation promotes oral and esophageal cancer stem cells via autophagy-mediated redox homeostasis

2020 ◽  
Author(s):  
Masataka Shimonosono ◽  
Takashi Kijima ◽  
Hisatsugu Maekawa ◽  
Satoshi Takada ◽  
Koji Tanaka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Esophageal Cancer ◽  
Cancer Stem Cells ◽  
Redox Homeostasis

scholarly journals Loss of miR-100 and miR-125b results in cancer stem cell properties through IGF2 upregulation in hepatocellular carcinoma

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hyang Sook Seol ◽  
Yoshimitsu Akiyama ◽  
San-Eun Lee ◽  
Shu Shimada ◽  
Se Jin Jang
Keyword(s):  
Hepatocellular Carcinoma ◽  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Xenograft Model ◽  
Mtor Pathway ◽  
Carcinoma Cells ◽  
Stable Cell Line ◽  
Hep3b Cells ◽  
Igf2 Expression

AbstractStemness factors control microRNA expression in cancer stem cells. Downregulation of miR-100 and miR-125b is associated with tumor progression and prognosis of various cancers. Comparing miRNA profiling of patient-derived tumorsphere (TS) and adherent (2D) hepatocellular carcinoma cells, miR-100 and miR-125b are identified to have association with stemness. In TS cells, miR-100 and miR-125b were downregulated comparing to 2D cells. The finding was reproduced in Hep3B cells. Overexpression of stemness factors NANOG, OCT4 and SOX2 by introduction of gene constructs in Hep3B cells suppressed these two miRNA expression levels. Treatment of chromeceptin, an IGF signaling pathway inhibitor, decreased numbers of TS and inhibited the AKT/mTOR pathway. Stable cell line of miR-100 and miR-125b overexpression decreased IGF2 expression and inhibited tumor growth in the xenograft model. In conclusion, miR-100 and miR-125b have tumor suppressor role in hepatocellular carcinoma through inhibiting IGF2 expression and activation of the AKT/mTOR pathway.

Esophageal cancer stem cells express PLGF to increase cancer invasion through MMP9 activation

Tumor Biology ◽  
2014 ◽  
Vol 35 (12) ◽  
pp. 12749-12755 ◽  
Author(s):  
Yue Chen ◽  
Tinghui Jiang ◽  
Aiwu Mao ◽  
Jianrong Xu
Keyword(s):  
Stem Cells ◽  
Esophageal Cancer ◽  
Cancer Stem Cells ◽  
Cancer Invasion ◽  
Mmp9 Activation

scholarly journals Etiology, cancer stem cells and potential diagnostic biomarkers for esophageal cancer

2019 ◽  
Vol 458 ◽  
pp. 21-28 ◽  
Author(s):  
Kuancan Liu ◽  
Tingting Zhao ◽  
Junkai Wang ◽  
Yunyun Chen ◽  
Rui Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Esophageal Cancer ◽  
Cancer Stem Cells ◽  
Diagnostic Biomarkers
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