scholarly journals Zebularine induces prolonged apoptosis effects via the caspase-3/PARP pathway in head and neck cancer cells

2014 ◽  
Vol 44 (6) ◽  
pp. 1971-1979 ◽  
Author(s):  
TINA NAPSO ◽  
FUAD FARES
Keyword(s):  
Head And Neck Cancer ◽  
Head And Neck ◽  
Cancer Cells ◽  
Neck Cancer ◽  
Caspase 3

scholarly journals Natural product triptolide induces GSDME-mediated pyroptosis in head and neck cancer through suppressing mitochondrial hexokinase-ΙΙ

2021 ◽  
Vol 40 (1) ◽  
Author(s):  
Jing Cai ◽  
Mei Yi ◽  
Yixin Tan ◽  
Xiaoling Li ◽  
Guiyuan Li ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Head And Neck ◽  
Cancer Cells ◽  
Natural Product ◽  
Neck Cancer ◽  
Caspase 3 ◽  
Chinese Herb ◽  
Mice Model ◽  
Hexokinase Ii ◽  
Western Blot Assay

Abstract Background Pyroptosis is a lytic cell death form executed by gasdermins family proteins. Induction of tumor pyroptosis promotes anti-tumor immunity and is a potential cancer treatment strategy. Triptolide (TPL) is a natural product isolated from the traditional Chinese herb which possesses potent anti-tumor activity in human cancers. However, its role in pyroptosis remains to be elucidated. Methods Cell survival was measured by colony formation assay. Cell apoptosis was determined by Annexin V assay. Pyroptosis was evaluated by morphological features and release of interleukin 1β and lactate dehydrogenase A (LDHA). Immunofluorescence staining was employed to measure subcellular localization of proteins. Tumorigenicity was assessed by a xenograft tumor model. Expression levels of mRNAs or proteins were determined by qPCR or western blot assay, respectively. Results Triptolide eliminates head and neck cancer cells through inducing gasdermin E (GSDME) mediated pyroptosis. Silencing GSDME attenuates the cytotoxicity of TPL against cancer cells. TPL treatment suppresses expression of c-myc and mitochondrial hexokinase II (HK-II) in cancer cells, leading to activation of the BAD/BAX-caspase 3 cascade and cleavage of GSDME by active caspase 3. Silencing HK-II sensitizes cancer cells to TPL induced pyroptosis, whereas enforced expression of HK-II prevents TPL induced pyroptosis. Mechanistically, HK-II prevents mitochondrial translocation of BAD, BAX proteins and activation of caspase 3, thus attenuating cleavage of GSDME and pyroptosis upon TPL treatment. Furthermore, TPL treatment suppresses NRF2/SLC7A11 (also known as xCT) axis and induces reactive oxygen species (ROS) accumulation, regardless of the status of GSDME. Combination of TPL with erastin, an inhibitor of SLC7A11, exerts robust synergistic effect in suppression of tumor survival in vitro and in a nude mice model. Conclusions This study not only provides a new paradigm of TPL in cancer therapy, but also highlights a crucial role of mitochondrial HK-II in linking glucose metabolism with pyroptosis.

scholarly journals Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro

2019 ◽  
Vol 60 (3) ◽  
pp. 289-297 ◽  
Author(s):  
Agata Abramowicz ◽  
Anna Wojakowska ◽  
Lukasz Marczak ◽  
Malgorzata Lysek-Gladysinska ◽  
Mateusz Smolarz ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Ionizing Radiation ◽  
Head And Neck ◽  
Cancer Cells ◽  
Extracellular Vesicles ◽  
Neck Cancer

IGF-1R-Mediated Resistance to EGFR Kinase Inhibitor PD158780 in Head and Neck Cancer Cells

2005 ◽  
Vol 133 (2) ◽  
pp. P95-P95
Author(s):  
M JAMESON ◽  
A BECKLER ◽  
L SANDSTROM ◽  
C THOMAS ◽  
P LEVINE
Keyword(s):  
Head And Neck Cancer ◽  
Head And Neck ◽  
Cancer Cells ◽  
Neck Cancer ◽  
Kinase Inhibitor ◽  
Egfr Kinase

scholarly journals TLR3 agonists improve the immunostimulatory potential of cetuximab against EGFR+head and neck cancer cells

2013 ◽  
Vol 2 (6) ◽  
pp. e24677 ◽  
Author(s):  
Chwee Ming Lim ◽  
Ryan Stephenson ◽  
Andres M Salazar ◽  
Robert L Ferris
Keyword(s):  
Head And Neck Cancer ◽  
Head And Neck ◽  
Cancer Cells ◽  
Neck Cancer

scholarly journals Down-regulation of multiple cell survival proteins in head and neck cancer cells by an apoptogenic mutant of adenovirus type 5

Virology ◽  
2009 ◽  
Vol 392 (1) ◽  
pp. 62-72 ◽  
Author(s):  
S. Vijayalingam ◽  
T. Subramanian ◽  
Jan Ryerse ◽  
Mark Varvares ◽  
G. Chinnadurai
Keyword(s):  
Head And Neck Cancer ◽  
Cell Survival ◽  
Head And Neck ◽  
Cancer Cells ◽  
Neck Cancer ◽  
Adenovirus Type ◽  
Down Regulation ◽  
Multiple Cell ◽  
Adenovirus Type 5 ◽  
Survival Proteins

scholarly journals Head and neck cancer cells and xenografts are very sensitive to palytoxin: decrease of c-jun n-terminale kinase-3 expression enhances palytoxin toxicity

2013 ◽  
Vol 12 (1) ◽  
pp. 12 ◽  
Author(s):  
Tibor Görögh ◽  
László Bèress ◽  
Elgar S Quabius ◽  
Petra Ambrosch ◽  
Markus Hoffmann
Keyword(s):  
Head And Neck Cancer ◽  
Head And Neck ◽  
Cancer Cells ◽  
Neck Cancer

scholarly journals CD44 exerts a functional role during EMT induction in cisplatin-resistant head and neck cancer cells

Oncotarget ◽  
2018 ◽  
Vol 9 (11) ◽  
pp. 10029-10041 ◽  
Author(s):  
Hiroaki Miyazaki ◽  
Ryou-u Takahashi ◽  
Marta Prieto-Vila ◽  
Yumi Kawamura ◽  
Seiji Kondo ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Head And Neck ◽  
Cancer Cells ◽  
Neck Cancer ◽  
Functional Role

scholarly journals 14-3-3 zeta is a molecular target in guggulsterone induced apoptosis in Head and Neck cancer cells

BMC Cancer ◽  
2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Muzafar A Macha ◽  
Ajay Matta ◽  
SS Chauhan ◽  
KW Michael Siu ◽  
Ranju Ralhan
Keyword(s):  
Head And Neck Cancer ◽  
Head And Neck ◽  
Cancer Cells ◽  
Neck Cancer ◽  
Molecular Target ◽  
Induced Apoptosis
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