scholarly journals High-resolution detection of recurrent aberrations in lung adenocarcinomas by array comparative genomic hybridization and expression analysis of selective genes by quantitative PCR

2014 ◽  
Vol 44 (6) ◽  
pp. 2068-2076 ◽  
Author(s):  
HONG ZHU ◽  
MARIA PIK WONG ◽  
VICKY TIN
Keyword(s):  
High Resolution ◽  
Quantitative Pcr ◽  
Comparative Genomic Hybridization ◽  
Expression Analysis ◽  
Array Comparative Genomic Hybridization ◽  
Comparative Genomic ◽  
Genomic Hybridization ◽  
Lung Adenocarcinomas

scholarly journals High-resolution array comparative genomic hybridization of single micrometastatic tumor cells

2008 ◽  
Vol 36 (7) ◽  
pp. e39 ◽  
Author(s):  
Christine Fuhrmann ◽  
Oleg Schmidt-Kittler ◽  
Nikolas H. Stoecklein ◽  
Karina Petat-Dutter ◽  
Christian Vay ◽  
...  
Keyword(s):  
High Resolution ◽  
Tumor Cells ◽  
Comparative Genomic Hybridization ◽  
Array Comparative Genomic Hybridization ◽  
Comparative Genomic ◽  
Genomic Hybridization

scholarly journals Aberrations in Pseudoautosomal Regions (PARs) Found in Infertile Men with Y-Chromosome Microdeletions

2011 ◽  
Vol 96 (4) ◽  
pp. E674-E679 ◽  
Author(s):  
Carolina J. Jorgez ◽  
John W. Weedin ◽  
Aysegul Sahin ◽  
Mounia Tannour-Louet ◽  
Shuo Han ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Quantitative Pcr ◽  
Comparative Genomic Hybridization ◽  
Array Comparative Genomic Hybridization ◽  
Structural Defects ◽  
Comparative Genomic ◽  
Genomic Hybridization ◽  
Infertile Men ◽  
Y Chromosome Microdeletions ◽  
Pseudoautosomal Regions

Abstract Context: The pseudoautosomal regions (PARs) of the Y-chromosome undergo meiotic recombination with the X-chromosome. PAR mutations are associated with infertility and mental and stature disorders. Objective: The aim of the study was to determine whether men with Y-chromosome microdeletions have structural defects in PARs. Design and Participants: Eighty-seven infertile men with Y-chromosome microdeletions and 35 controls were evaluated for chromosomal rearrangements using commercial or custom (X- and Y-chromosome) array comparative genomic hybridization or by quantitative PCR of selected PAR genes. Multisoftware-defined chromosomal gains or losses were validated by quantitative PCR and FISH. Results: Array comparative genomic hybridization confirmed the AZF deletions identified by multiplex PCR. All men with Y-chromosome microdeletions and an abnormal karyotype displayed PAR abnormalities, as did 10% of men with Y-chromosome microdeletions and a normal karyotype. None of the control subjects or infertile men without Y-chromosome microdeletions had PAR duplications or deletions. SHOX aberrations occurred in 14 men (nine gains and five losses); four were short in stature (<10th percentile), and one was tall (>95th percentile). In contrast, the height of 23 men with Y-chromosome microdeletions and normal PARs was average at 176.8 cm (50th percentile). Conclusions: Y-chromosome microdeletions can include PAR defects causing genomic disorders such as SHOX, which may be transmitted to offspring. Previously unrecognized PAR gains and losses in men with Y-chromosome microdeletions may have consequences for offspring.

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