scholarly journals JS-K, a nitric oxide-releasing prodrug, induces breast cancer cell death while sparing normal mammary epithelial cells

2011 ◽  
Vol 38 (4) ◽  
Author(s):  
Tari
Keyword(s):  
Breast Cancer ◽  
Nitric Oxide ◽  
Cell Death ◽  
Epithelial Cells ◽  
Breast Cancer Cell ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Cancer Cell Death ◽  
Nitric Oxide Releasing ◽  
Normal Mammary Epithelial

scholarly journals Statin-Induced Breast Cancer Cell Death: Role of Inducible Nitric Oxide and Arginase-Dependent Pathways

2007 ◽  
Vol 67 (15) ◽  
pp. 7386-7394 ◽  
Author(s):  
Srigiridhar Kotamraju ◽  
Carol L. Willams ◽  
Balaraman Kalyanaraman
Keyword(s):  
Breast Cancer ◽  
Nitric Oxide ◽  
Cell Death ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Death ◽  
Inducible Nitric Oxide

Essential role of biliary glycoprotein (CD66a) in morphogenesis of the human mammary epithelial cell line MCF10F

1999 ◽  
Vol 112 (23) ◽  
pp. 4193-4205 ◽  
Author(s):  
J. Huang ◽  
J.D. Hardy ◽  
Y. Sun ◽  
J.E. Shively
Keyword(s):  
Breast Cancer ◽  
Cell Death ◽  
Epithelial Cells ◽  
Cell Line ◽  
Myoepithelial Cell ◽  
Mammary Epithelial Cells ◽  
Tumor Cell Line ◽  
Mammary Epithelial ◽  
Cell Phenotype ◽  
Epithelial Cell Line

Normal mammary epithelial cells express the cell surface protein biliary glycoprotein (BGP or CD66a) in a polarized manner, suggesting that this protein may play a role in the formation of mammary acini. In order to test this hypothesis, we interrupted the expression of BGP in the mammary epithelial line MCF10F when cultured in or on Matrigel, a source of extracellular matrix (ECM). When analyzed by immunofluorescence confocal microscopy, the BGP staining is confined to the lumenal surface and colocalizes with actin. Sequential scanning electron microscopy demonstrates that the MCF10F cells migrate to form clusters, followed by apoptotic cell death within the center, resulting in lumen formation. Transmission electron micrographs reveal the presence of tight junctions and desmosomes between the cells, microvilli along the lumenal surface, and typical apoptotic bodies within the lumen. When the MCF10F cells are transfected with the BGP antisense gene and grown in Matrigel, they exhibit reduced acini formation (12% and 20%) compared to untransfected cells (52%) or to cells transfected with vector only (62%). Acini formation is also significantly reduced when MCF10F cells grown in Matrigel are treated with anti-BGP antibody (18% at 100 microgram/ml), or recombinant soluble BGP (18% at 0.4 microM). In contrast, the BGP-negative MCF7 breast tumor cell line, which does not form acini when grown in matrigel, exhibits >60% cell death with the occasional formation of acini, when transfected with the BGP sense gene and grown in Matrigel. These results support the hypothesis that BGP plays a role in the normal differentiation program of mammary epithelial cells, indicating that its expression is essential to the formation of the lumen. Furthermore, and as shown by others, the differentiation program depends on the presence of ECM. The lack of expression of BGP in the MCF7 breast cancer cell line suggests that the downregulation of BGP expression confers a growth advantage to these cells in ECM. In addition, we found that the MCF10F cells could be separated into a BGP-positive epithelial fraction (MCF10F-e), and a BGP-negative myoepithelial fraction (MCF10F-m). When the myoepithelial cell-enriched fraction is grown on Matrigel, web-like structures are formed. These cells have a typical spindle shape cell morphology and express keratin, alpha-smooth muscle actin and vimentin, markers of the myoepithelial cell phenotype. When MCF10F-m cells are treated with IFNgamma, they express CEA (carcinoembryonic antigen) but not BGP. Since breast carcinomas, especially in situ carcinomas, express CEA, this finding may suggest a heretofore unappreciated relationship between myoepithelial cells and breast cancer.

2-DE analysis of a new human cell line EM-G3 derived from breast cancer progenitor cells and comparison with normal mammary epithelial cells

PROTEOMICS ◽  
2007 ◽  
Vol 7 (9) ◽  
pp. 1549-1559 ◽  
Author(s):  
Irena Selicharová ◽  
Kateřina Smutná ◽  
Miloslav Šanda ◽  
Karel Ubik ◽  
Eva Matoušková ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Epithelial Cells ◽  
Cell Line ◽  
Progenitor Cells ◽  
Human Cell ◽  
Mammary Epithelial Cells ◽  
Human Cell Line ◽  
Mammary Epithelial ◽  
Normal Mammary Epithelial
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